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Occurrence of bla CTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia.

Abstract

Avian ESBL-producing Escherichia coli isolates have been increasingly reported worldwide. Animal to human dissemination, via food chain or direct contact, of these resistant bacteria has been reported. In Tunisia, little is known about avian ESBL- producing E. coli and further studies are needed. Seventeen ESBL-producing Escherichia coli isolates from poultry feces from two farms (Farm 1 and farm 2) in the North of Tunisia have been used in this study. Eleven of these isolates (from farm 1) have the same resistance profile to nalidixic acid, sulfonamides, streptomycin, tetracycline, and norfloxacine (intermediately resistant). Out of the six isolates recovered from farm 2, only one was co-resistant to tetracycline. All isolates, except one, harbored bla CTX-M-1 gene, and one strain co-harbored the bla TEM-1 gene. The genes tetA and tetB were carried, respectively, by 11 and 1 amongst the 12 tetracycline-resistant isolates. Sulfonamides resistance was encoded by sul1, sul2, and sul3 genes in 3, 17, and 5 isolates, respectively. The qnrB1 was detected in nine strains, one of which co-harbored qnrS1 gene. The search for the class 1 and 2 integrons by PCR showed that in farm 1, class 1 and 2 integrons were found in one and ten isolates, respectively. In farm 2, class 1 integron was found in only one isolate, class 2 was not detected. Only one gene cassette arrangement was demonstrated in the variable regions (VR) of the 10 int2-positive isolates: dfrA1- sat2-aadA1. The size of the VR of the class 1 integron was approximately 250 bp in one int1-positive isolate, whereas in the second isolate, no amplification was observed. All isolates of farm 1 belong to the phylogroup A (sub-group A0). However, different types of phylogroups in farm 2 were detected. Each of the phylogroups A1, B22, B23 was detected in one strain, while the D2 phylogroup was found in 3 isolates. The virulence genes iutA, fimH, and traT were detected in 3, 7, and 3 isolates, respectively. Two types of gene combination were detected: iutA+fimH+traT in 3 isolates and iutA+fimH in one isolate. The isolates recovered in farm 1 showed the same profile of PFGE macro-restriction, while isolates of farm 2 presented unrelated PFGE patterns. We conclude that these avian ESBL-producing E. coli isolates show homo- and heterogenic genetic background and that plasmids harboring ESBL genes could be involved in the dissemination of this resistance phenotype.

Authors+Show Affiliations

Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia ; LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Faculté de Médecine de Tunis, Université de Tunis El Manar Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia ; LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Faculté de Médecine de Tunis, Université de Tunis El Manar Tunis, Tunisia.LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Faculté de Médecine de Tunis, Université de Tunis El Manar Tunis, Tunisia ; Hôpital Charles Nicolle, Service de Microbiologie Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia ; Regional Animal Health Center for North Africa (RAHC-NA) Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia.École Nationale de Médecine Vétérinaire de Sidi Thabet Sidi Thabet, Tunisia.Laboratory of Bacteriological Research, Institut de la Recherche Vétérinaire de Tunis, Université de Tunis El Manar Tunis, Tunisia.LR99ES09 Laboratoire de Résistance aux Antimicrobiens, Faculté de Médecine de Tunis, Université de Tunis El Manar Tunis, Tunisia ; Hôpital Charles Nicolle, Service de Microbiologie Tunis, Tunisia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26000252

Citation

Kilani, Hajer, et al. "Occurrence of Bla CTX-M-1, qnrB1 and Virulence Genes in Avian ESBL-producing Escherichia Coli Isolates From Tunisia." Frontiers in Cellular and Infection Microbiology, vol. 5, 2015, p. 38.
Kilani H, Abbassi MS, Ferjani S, et al. Occurrence of bla CTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia. Front Cell Infect Microbiol. 2015;5:38.
Kilani, H., Abbassi, M. S., Ferjani, S., Mansouri, R., Sghaier, S., Ben Salem, R., Jaouani, I., Douja, G., Brahim, S., Hammami, S., Ben Chehida, N., & Boubaker, I. B. (2015). Occurrence of bla CTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia. Frontiers in Cellular and Infection Microbiology, 5, 38. https://doi.org/10.3389/fcimb.2015.00038
Kilani H, et al. Occurrence of Bla CTX-M-1, qnrB1 and Virulence Genes in Avian ESBL-producing Escherichia Coli Isolates From Tunisia. Front Cell Infect Microbiol. 2015;5:38. PubMed PMID: 26000252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of bla CTX-M-1, qnrB1 and virulence genes in avian ESBL-producing Escherichia coli isolates from Tunisia. AU - Kilani,Hajer, AU - Abbassi,Mohamed Salah, AU - Ferjani,Sana, AU - Mansouri,Riadh, AU - Sghaier,Senda, AU - Ben Salem,Rakia, AU - Jaouani,Imen, AU - Douja,Gtari, AU - Brahim,Sana, AU - Hammami,Salah, AU - Ben Chehida,Noureddine, AU - Boubaker,Ilhem Boutiba-Ben, Y1 - 2015/05/05/ PY - 2015/01/15/received PY - 2015/04/13/accepted PY - 2015/5/23/entrez PY - 2015/5/23/pubmed PY - 2016/1/20/medline KW - Escherichia coli KW - blaCTX-M-1 KW - clonality KW - integrons KW - poultry KW - qnrB1 SP - 38 EP - 38 JF - Frontiers in cellular and infection microbiology JO - Front Cell Infect Microbiol VL - 5 N2 - Avian ESBL-producing Escherichia coli isolates have been increasingly reported worldwide. Animal to human dissemination, via food chain or direct contact, of these resistant bacteria has been reported. In Tunisia, little is known about avian ESBL- producing E. coli and further studies are needed. Seventeen ESBL-producing Escherichia coli isolates from poultry feces from two farms (Farm 1 and farm 2) in the North of Tunisia have been used in this study. Eleven of these isolates (from farm 1) have the same resistance profile to nalidixic acid, sulfonamides, streptomycin, tetracycline, and norfloxacine (intermediately resistant). Out of the six isolates recovered from farm 2, only one was co-resistant to tetracycline. All isolates, except one, harbored bla CTX-M-1 gene, and one strain co-harbored the bla TEM-1 gene. The genes tetA and tetB were carried, respectively, by 11 and 1 amongst the 12 tetracycline-resistant isolates. Sulfonamides resistance was encoded by sul1, sul2, and sul3 genes in 3, 17, and 5 isolates, respectively. The qnrB1 was detected in nine strains, one of which co-harbored qnrS1 gene. The search for the class 1 and 2 integrons by PCR showed that in farm 1, class 1 and 2 integrons were found in one and ten isolates, respectively. In farm 2, class 1 integron was found in only one isolate, class 2 was not detected. Only one gene cassette arrangement was demonstrated in the variable regions (VR) of the 10 int2-positive isolates: dfrA1- sat2-aadA1. The size of the VR of the class 1 integron was approximately 250 bp in one int1-positive isolate, whereas in the second isolate, no amplification was observed. All isolates of farm 1 belong to the phylogroup A (sub-group A0). However, different types of phylogroups in farm 2 were detected. Each of the phylogroups A1, B22, B23 was detected in one strain, while the D2 phylogroup was found in 3 isolates. The virulence genes iutA, fimH, and traT were detected in 3, 7, and 3 isolates, respectively. Two types of gene combination were detected: iutA+fimH+traT in 3 isolates and iutA+fimH in one isolate. The isolates recovered in farm 1 showed the same profile of PFGE macro-restriction, while isolates of farm 2 presented unrelated PFGE patterns. We conclude that these avian ESBL-producing E. coli isolates show homo- and heterogenic genetic background and that plasmids harboring ESBL genes could be involved in the dissemination of this resistance phenotype. SN - 2235-2988 UR - https://www.unboundmedicine.com/medline/citation/26000252/Occurrence_of_bla_CTX_M_1_qnrB1_and_virulence_genes_in_avian_ESBL_producing_Escherichia_coli_isolates_from_Tunisia_ L2 - https://doi.org/10.3389/fcimb.2015.00038 DB - PRIME DP - Unbound Medicine ER -