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Plasma and serum serotonin concentrations and surface-bound platelet serotonin expression in Cavalier King Charles Spaniels with myxomatous mitral valve disease.
Am J Vet Res. 2015 Jun; 76(6):520-31.AJ

Abstract

OBJECTIVE

To investigate serum and plasma serotonin concentrations, percentage of serotonin-positive platelets, level of surface-bound platelet serotonin expression (mean fluorescence intensity [MFI]), and platelet activation (CD62 expression) in platelet-rich plasma from Cavalier King Charles Spaniels with myxomatous mitral valve disease (MMVD).

ANIMALS

Healthy dogs (n = 15) and dogs with mild MMVD (18), moderate-severe MMVD (19), or severe MMVD with congestive heart failure (CHF; 10).

PROCEDURES

Blood samples were collected from each dog. Serum and plasma serotonin concentrations were measured with an ELISA, and surface-bound platelet serotonin expression and platelet activation were determined by flow cytometry.

RESULTS

Dogs with mild MMVD had higher median serum (746 ng/mL) and plasma (33.3 ng/mL) serotonin concentrations, compared with MMVD-affected dogs with CHF (388 ng/mL and 9.9 ng/mL, respectively), but no other group differences were found. Among disease groups, no differences in surface-bound serotonin expression or platelet activation were found. Thrombocytopenic dogs had lower serum serotonin concentration (482 ng/mL) than nonthrombocytopenic dogs (731 ng/mL). In 26 dogs, a flow cytometry scatterplot subpopulation (FSSP) of platelets was identified; dogs with an FSSP had a higher percentage of serotonin-positive platelets (11.0%), higher level of surface-bound serotonin expression (MFI, 32,068), and higher platelet activation (MFI, 2,363) than did dogs without an FSSP (5.7%, 1,230, and 1,165, respectively). An FSSP was present in 93.8% of thrombocytopenic dogs and in 29.5% of nonthrombocytopenic dogs.

CONCLUSIONS AND CLINICAL RELEVANCE

A substantive influence of circulating serotonin on MMVD stages prior to CHF development in Cavalier King Charles Spaniels was not supported by the study findings. An FSSP of highly activated platelets with pronounced serotonin binding was strongly associated with thrombocytopenia but not MMVD.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26000599

Citation

Cremer, Signe E., et al. "Plasma and Serum Serotonin Concentrations and Surface-bound Platelet Serotonin Expression in Cavalier King Charles Spaniels With Myxomatous Mitral Valve Disease." American Journal of Veterinary Research, vol. 76, no. 6, 2015, pp. 520-31.
Cremer SE, Kristensen AT, Reimann MJ, et al. Plasma and serum serotonin concentrations and surface-bound platelet serotonin expression in Cavalier King Charles Spaniels with myxomatous mitral valve disease. Am J Vet Res. 2015;76(6):520-31.
Cremer, S. E., Kristensen, A. T., Reimann, M. J., Eriksen, N. B., Petersen, S. F., Marschner, C. B., Tarnow, I., Oyama, M. A., & Olsen, L. H. (2015). Plasma and serum serotonin concentrations and surface-bound platelet serotonin expression in Cavalier King Charles Spaniels with myxomatous mitral valve disease. American Journal of Veterinary Research, 76(6), 520-31. https://doi.org/10.2460/ajvr.76.6.520
Cremer SE, et al. Plasma and Serum Serotonin Concentrations and Surface-bound Platelet Serotonin Expression in Cavalier King Charles Spaniels With Myxomatous Mitral Valve Disease. Am J Vet Res. 2015;76(6):520-31. PubMed PMID: 26000599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma and serum serotonin concentrations and surface-bound platelet serotonin expression in Cavalier King Charles Spaniels with myxomatous mitral valve disease. AU - Cremer,Signe E, AU - Kristensen,Annemarie T, AU - Reimann,Maria J, AU - Eriksen,Nynne B, AU - Petersen,Stine F, AU - Marschner,Clara B, AU - Tarnow,Inge, AU - Oyama,Mark A, AU - Olsen,Lisbeth H, PY - 2015/5/23/entrez PY - 2015/5/23/pubmed PY - 2015/11/18/medline SP - 520 EP - 31 JF - American journal of veterinary research JO - Am. J. Vet. Res. VL - 76 IS - 6 N2 - OBJECTIVE: To investigate serum and plasma serotonin concentrations, percentage of serotonin-positive platelets, level of surface-bound platelet serotonin expression (mean fluorescence intensity [MFI]), and platelet activation (CD62 expression) in platelet-rich plasma from Cavalier King Charles Spaniels with myxomatous mitral valve disease (MMVD). ANIMALS: Healthy dogs (n = 15) and dogs with mild MMVD (18), moderate-severe MMVD (19), or severe MMVD with congestive heart failure (CHF; 10). PROCEDURES: Blood samples were collected from each dog. Serum and plasma serotonin concentrations were measured with an ELISA, and surface-bound platelet serotonin expression and platelet activation were determined by flow cytometry. RESULTS: Dogs with mild MMVD had higher median serum (746 ng/mL) and plasma (33.3 ng/mL) serotonin concentrations, compared with MMVD-affected dogs with CHF (388 ng/mL and 9.9 ng/mL, respectively), but no other group differences were found. Among disease groups, no differences in surface-bound serotonin expression or platelet activation were found. Thrombocytopenic dogs had lower serum serotonin concentration (482 ng/mL) than nonthrombocytopenic dogs (731 ng/mL). In 26 dogs, a flow cytometry scatterplot subpopulation (FSSP) of platelets was identified; dogs with an FSSP had a higher percentage of serotonin-positive platelets (11.0%), higher level of surface-bound serotonin expression (MFI, 32,068), and higher platelet activation (MFI, 2,363) than did dogs without an FSSP (5.7%, 1,230, and 1,165, respectively). An FSSP was present in 93.8% of thrombocytopenic dogs and in 29.5% of nonthrombocytopenic dogs. CONCLUSIONS AND CLINICAL RELEVANCE: A substantive influence of circulating serotonin on MMVD stages prior to CHF development in Cavalier King Charles Spaniels was not supported by the study findings. An FSSP of highly activated platelets with pronounced serotonin binding was strongly associated with thrombocytopenia but not MMVD. SN - 1943-5681 UR - https://www.unboundmedicine.com/medline/citation/26000599/Plasma_and_serum_serotonin_concentrations_and_surface_bound_platelet_serotonin_expression_in_Cavalier_King_Charles_Spaniels_with_myxomatous_mitral_valve_disease_ L2 - http://avmajournals.avma.org/doi/full/10.2460/ajvr.76.6.520?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -