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Quantitative determination of fucoidan using polyion-sensitive membrane electrodes.
Anal Chim Acta 2015; 877:1-8AC

Abstract

The use of polyanion and polycation-sensitive membrane electrodes to detect five different preparations of fucoidan is described. Unlike linear polyanionic molecules previously measured with polymer membrane-based electrochemical sensors, fucoidans from marine brown algae are all highly branched, sulfated polysaccharides with varying charge densities and structures, depending on the species of seaweed, method of extraction used and extent of purification. When tridodecylmethylammonium (TDMA) was used as the ion-exchanger, a large, non-equilibrium EMF response was observed over a concentration range of 0.5-50 μg mL(-1) fucoidan. Fucoidan was also measured by titration with polycationic protamine, using a dinonylnaphthalene sulfonate (DNNS)-doped membrane electrode as the potentiometric endpoint detector. Potentiometric titration was used to determine the binding ratio between protamine and fucoidan at the neutralization endpoint for each fucoidan preparation. This binding ratio was then used to successfully determine the fucoidan content of commercially available nutritional supplements. Fucoidan was also measured in undiluted blood serum, demonstrating that this method may be applicable for measuring fucoidan for clinical applications.

Authors+Show Affiliations

Department of Chemistry, Eastern University, 1300 Eagle Rd., St. Davids, PA 19087, United States.Department of Chemistry, Eastern University, 1300 Eagle Rd., St. Davids, PA 19087, United States.Department of Chemistry, Eastern University, 1300 Eagle Rd., St. Davids, PA 19087, United States.Department of Chemistry, Eastern University, 1300 Eagle Rd., St. Davids, PA 19087, United States.Marinova Pty Ltd., 249 Kennedy Drive, Cambridge, Tasmania 7170, Australia.Marinova Pty Ltd., 249 Kennedy Drive, Cambridge, Tasmania 7170, Australia.Department of Chemistry, Eastern University, 1300 Eagle Rd., St. Davids, PA 19087, United States. Electronic address: kmowery@eastern.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26002205

Citation

Kim, Ji Min, et al. "Quantitative Determination of Fucoidan Using Polyion-sensitive Membrane Electrodes." Analytica Chimica Acta, vol. 877, 2015, pp. 1-8.
Kim JM, Nguyen L, Barr MF, et al. Quantitative determination of fucoidan using polyion-sensitive membrane electrodes. Anal Chim Acta. 2015;877:1-8.
Kim, J. M., Nguyen, L., Barr, M. F., Morabito, M., Stringer, D., Fitton, J. H., & Mowery, K. A. (2015). Quantitative determination of fucoidan using polyion-sensitive membrane electrodes. Analytica Chimica Acta, 877, pp. 1-8. doi:10.1016/j.aca.2015.04.020.
Kim JM, et al. Quantitative Determination of Fucoidan Using Polyion-sensitive Membrane Electrodes. Anal Chim Acta. 2015 Jun 2;877:1-8. PubMed PMID: 26002205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative determination of fucoidan using polyion-sensitive membrane electrodes. AU - Kim,Ji Min, AU - Nguyen,Loc, AU - Barr,Mary Frances, AU - Morabito,Michael, AU - Stringer,Damien, AU - Fitton,J Helen, AU - Mowery,Kelly A, Y1 - 2015/04/17/ PY - 2015/02/04/received PY - 2015/04/09/revised PY - 2015/04/10/accepted PY - 2015/5/24/entrez PY - 2015/5/24/pubmed PY - 2016/2/13/medline KW - Fucoidan KW - Polyion-sensitive electrode KW - Potentiometric titration KW - Potentiometry SP - 1 EP - 8 JF - Analytica chimica acta JO - Anal. Chim. Acta VL - 877 N2 - The use of polyanion and polycation-sensitive membrane electrodes to detect five different preparations of fucoidan is described. Unlike linear polyanionic molecules previously measured with polymer membrane-based electrochemical sensors, fucoidans from marine brown algae are all highly branched, sulfated polysaccharides with varying charge densities and structures, depending on the species of seaweed, method of extraction used and extent of purification. When tridodecylmethylammonium (TDMA) was used as the ion-exchanger, a large, non-equilibrium EMF response was observed over a concentration range of 0.5-50 μg mL(-1) fucoidan. Fucoidan was also measured by titration with polycationic protamine, using a dinonylnaphthalene sulfonate (DNNS)-doped membrane electrode as the potentiometric endpoint detector. Potentiometric titration was used to determine the binding ratio between protamine and fucoidan at the neutralization endpoint for each fucoidan preparation. This binding ratio was then used to successfully determine the fucoidan content of commercially available nutritional supplements. Fucoidan was also measured in undiluted blood serum, demonstrating that this method may be applicable for measuring fucoidan for clinical applications. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/26002205/Quantitative_determination_of_fucoidan_using_polyion_sensitive_membrane_electrodes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(15)00514-0 DB - PRIME DP - Unbound Medicine ER -