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Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte.
Insect Biochem Mol Biol. 2015 Aug; 63:54-62.IB

Abstract

RNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult females. dsRNA feeding caused a significant decrease in hb and brm transcripts in the adult females. Although total oviposition was not significantly affected, there was almost complete absence of hatching in the eggs collected from females exposed to dsRNA for either gene. These results confirm that RNAi is systemic in nature for western corn rootworms. These results also indicate that hunchback and brahma play important roles in rootworm embryonic development and could provide useful RNAi targets in adult rootworms to prevent crop injury by impacting the population of larval progeny of exposed adults. The ability to deliver dsRNA in a trans-generational manner by feeding to adult rootworms may offer an additional approach to utilizing RNAi for rootworm pest management. The potential to develop parental RNAi technology targeting progeny of adult rootworms in combination with Bt proteins or dsRNA lethal to larvae may increase opportunities to develop sustainable approaches to rootworm management involving RNAi technologies for rootworm control.

Authors+Show Affiliations

University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.Embrapa Maize and Sorghum, Rodovia MG 424, Sete Lagoas, MG, Brazil.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, United States.University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States. Electronic address: bsiegfried1@unl.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26005118

Citation

Khajuria, Chitvan, et al. "Parental RNA Interference of Genes Involved in Embryonic Development of the Western Corn Rootworm, Diabrotica Virgifera Virgifera LeConte." Insect Biochemistry and Molecular Biology, vol. 63, 2015, pp. 54-62.
Khajuria C, Vélez AM, Rangasamy M, et al. Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte. Insect Biochem Mol Biol. 2015;63:54-62.
Khajuria, C., Vélez, A. M., Rangasamy, M., Wang, H., Fishilevich, E., Frey, M. L., Carneiro, N. P., Gandra, P., Narva, K. E., & Siegfried, B. D. (2015). Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte. Insect Biochemistry and Molecular Biology, 63, 54-62. https://doi.org/10.1016/j.ibmb.2015.05.011
Khajuria C, et al. Parental RNA Interference of Genes Involved in Embryonic Development of the Western Corn Rootworm, Diabrotica Virgifera Virgifera LeConte. Insect Biochem Mol Biol. 2015;63:54-62. PubMed PMID: 26005118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parental RNA interference of genes involved in embryonic development of the western corn rootworm, Diabrotica virgifera virgifera LeConte. AU - Khajuria,Chitvan, AU - Vélez,Ana M, AU - Rangasamy,Murugesan, AU - Wang,Haichuan, AU - Fishilevich,Elane, AU - Frey,Meghan L F, AU - Carneiro,Newton Portilho, AU - Gandra,Premchand, AU - Narva,Kenneth E, AU - Siegfried,Blair D, Y1 - 2015/05/22/ PY - 2015/02/10/received PY - 2015/05/09/revised PY - 2015/05/16/accepted PY - 2015/5/26/entrez PY - 2015/5/26/pubmed PY - 2016/5/28/medline KW - Brahma KW - Hunchback KW - RNAi KW - Western corn rootworm KW - pRNAi SP - 54 EP - 62 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 63 N2 - RNA interference (RNAi) is being developed as a potential tool for insect pest management and one of the most likely target pest species for transgenic plants that express double stranded RNA (dsRNA) is the western corn rootworm. Thus far, most genes proposed as targets for RNAi in rootworm cause lethality in the larval stage. In this study, we describe RNAi-mediated knockdown of two developmental genes, hunchback (hb) and brahma (brm), in the western corn rootworm delivered via dsRNA fed to adult females. dsRNA feeding caused a significant decrease in hb and brm transcripts in the adult females. Although total oviposition was not significantly affected, there was almost complete absence of hatching in the eggs collected from females exposed to dsRNA for either gene. These results confirm that RNAi is systemic in nature for western corn rootworms. These results also indicate that hunchback and brahma play important roles in rootworm embryonic development and could provide useful RNAi targets in adult rootworms to prevent crop injury by impacting the population of larval progeny of exposed adults. The ability to deliver dsRNA in a trans-generational manner by feeding to adult rootworms may offer an additional approach to utilizing RNAi for rootworm pest management. The potential to develop parental RNAi technology targeting progeny of adult rootworms in combination with Bt proteins or dsRNA lethal to larvae may increase opportunities to develop sustainable approaches to rootworm management involving RNAi technologies for rootworm control. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/26005118/Parental_RNA_interference_of_genes_involved_in_embryonic_development_of_the_western_corn_rootworm_Diabrotica_virgifera_virgifera_LeConte_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(15)00101-0 DB - PRIME DP - Unbound Medicine ER -