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Fabrication of chitosan microspheres using vanillin/TPP dual crosslinkers for protein antigens encapsulation.
Carbohydr Polym. 2015 Sep 05; 128:188-98.CP

Abstract

Microspheres were prepared from water soluble chitosan using dual vanillin/TPP crosslinkers. Placebo (C1), Bovine serum albumin (BSA) (C2), monovalent tetanus toxoid (TT) (C3) and divalent tetanus (TT) and diphtheria toxoids (DT) (C4) encapsulated microspheres were studied in terms of size (1-4 μm), encapsulation efficiency (75-80%), swelling and mucoadhesion (56-68%). FT-IR, TGA, XRD and SEM characterization of microspheres suggested specific interaction, more thermal stability, amorphous nature and rough surfaces of encapsulated microspheres. EDS confirmed the co-crosslinking and ninhydrin tests were showing higher crosslinking density. Zeta potential was 47.7 to 66.2 +mV indicating the potential stability of the colloidal system. Equilibrium adsorption isotherms described encapsulated microspheres followed the Langmuir isotherm model, suggesting monolayer adsorption of the mucin on microspheres. In-vitro release studies up to four weeks indicated zero order kinetics and obeyed swelling-controlled super case II transport release mechanism. Thus, the present study could be helpful in developing the multivalent oral vaccine.

Authors+Show Affiliations

Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.Toxoid Purification Department, Serum Institute of India Ltd., Hadapsar, Pune 411028, India.Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University (Formerly University of Pune), Pune 411007, India. Electronic address: pdoshi@chem.unipune.ac.in.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26005155

Citation

Walke, Shilratna, et al. "Fabrication of Chitosan Microspheres Using vanillin/TPP Dual Crosslinkers for Protein Antigens Encapsulation." Carbohydrate Polymers, vol. 128, 2015, pp. 188-98.
Walke S, Srivastava G, Nikalje M, et al. Fabrication of chitosan microspheres using vanillin/TPP dual crosslinkers for protein antigens encapsulation. Carbohydr Polym. 2015;128:188-98.
Walke, S., Srivastava, G., Nikalje, M., Doshi, J., Kumar, R., Ravetkar, S., & Doshi, P. (2015). Fabrication of chitosan microspheres using vanillin/TPP dual crosslinkers for protein antigens encapsulation. Carbohydrate Polymers, 128, 188-98. https://doi.org/10.1016/j.carbpol.2015.04.020
Walke S, et al. Fabrication of Chitosan Microspheres Using vanillin/TPP Dual Crosslinkers for Protein Antigens Encapsulation. Carbohydr Polym. 2015 Sep 5;128:188-98. PubMed PMID: 26005155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fabrication of chitosan microspheres using vanillin/TPP dual crosslinkers for protein antigens encapsulation. AU - Walke,Shilratna, AU - Srivastava,Gopal, AU - Nikalje,Milind, AU - Doshi,Jignesh, AU - Kumar,Rakesh, AU - Ravetkar,Satish, AU - Doshi,Pooja, Y1 - 2015/04/21/ PY - 2015/03/04/received PY - 2015/04/08/revised PY - 2015/04/13/accepted PY - 2015/5/26/entrez PY - 2015/5/26/pubmed PY - 2016/2/24/medline KW - Co-crosslinking KW - Microspheres KW - Protein antigens KW - TPP KW - Vanillin KW - Water soluble chitosan SP - 188 EP - 98 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 128 N2 - Microspheres were prepared from water soluble chitosan using dual vanillin/TPP crosslinkers. Placebo (C1), Bovine serum albumin (BSA) (C2), monovalent tetanus toxoid (TT) (C3) and divalent tetanus (TT) and diphtheria toxoids (DT) (C4) encapsulated microspheres were studied in terms of size (1-4 μm), encapsulation efficiency (75-80%), swelling and mucoadhesion (56-68%). FT-IR, TGA, XRD and SEM characterization of microspheres suggested specific interaction, more thermal stability, amorphous nature and rough surfaces of encapsulated microspheres. EDS confirmed the co-crosslinking and ninhydrin tests were showing higher crosslinking density. Zeta potential was 47.7 to 66.2 +mV indicating the potential stability of the colloidal system. Equilibrium adsorption isotherms described encapsulated microspheres followed the Langmuir isotherm model, suggesting monolayer adsorption of the mucin on microspheres. In-vitro release studies up to four weeks indicated zero order kinetics and obeyed swelling-controlled super case II transport release mechanism. Thus, the present study could be helpful in developing the multivalent oral vaccine. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/26005155/Fabrication_of_chitosan_microspheres_using_vanillin/TPP_dual_crosslinkers_for_protein_antigens_encapsulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(15)00329-X DB - PRIME DP - Unbound Medicine ER -