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Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies.
ACS Appl Mater Interfaces. 2015 Jun 17; 7(23):12663-70.AA

Abstract

A novel electrochemiluminescent (ECL) aptasensor was proposed for the determination of thrombin (TB) using exonuclease-catalyzed target recycling and hybridization chain reaction (HCR) to amplify the signal. The capture probe was immobilized on an Au-GS-modified electrode through a Au-S bond. Subsequently, the hybrid between the capture probe and the complementary thrombin binding aptamer (TBA) was aimed at obtaining double-stranded DNA (dsDNA). The interaction between TB and its aptamer led to the dissociation of dsDNA because TB has a higher affinity to TBA than the complementary strands. In the presence of exonuclease, aptamer was selectively digested and TB could be released for target recycling. Extended dsDNA was formed through HCR of the capture probe and two hairpin DNA strands (NH2-DNA1 and NH2-DNA1). Then, numerous europium multiwalled carbon nanotubes (Eu-MWCNTs) could be introduced through amidation reaction between NH2-terminated DNA strands and carboxyl groups on the Eu-MWCNTs, resulting in an increased ECL signal. The multiple amplification strategies, including the amplification of analyte recycling and HCR, and high ECL efficiency of Eu-MWCNTs lead to a wide linear range (1.0×10(-12)-5.0×10(-9) mol/L) and a low detection limit (0.23 pmol/L). The method was applied to serum sample analysis with satisfactory results.

Authors+Show Affiliations

No affiliation info available‡National Engineering Technology Research Center for Colloidal Materials, Shandong University, Jinan 250100, China.No affiliation info available§Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.§Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26005759

Citation

Wu, Dan, et al. "Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies." ACS Applied Materials & Interfaces, vol. 7, no. 23, 2015, pp. 12663-70.
Wu D, Xin X, Pang X, et al. Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies. ACS Appl Mater Interfaces. 2015;7(23):12663-70.
Wu, D., Xin, X., Pang, X., Pietraszkiewicz, M., Hozyst, R., Sun, X., & Wei, Q. (2015). Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies. ACS Applied Materials & Interfaces, 7(23), 12663-70. https://doi.org/10.1021/acsami.5b03381
Wu D, et al. Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies. ACS Appl Mater Interfaces. 2015 Jun 17;7(23):12663-70. PubMed PMID: 26005759.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of Europium Multiwalled Carbon Nanotubes as Novel Luminophores in an Electrochemiluminescent Aptasensor for Thrombin Using Multiple Amplification Strategies. AU - Wu,Dan, AU - Xin,Xia, AU - Pang,Xuehui, AU - Pietraszkiewicz,Marek, AU - Hozyst,Robert, AU - Sun,Xian'ge, AU - Wei,Qin, Y1 - 2015/06/02/ PY - 2015/5/26/entrez PY - 2015/5/26/pubmed PY - 2016/4/15/medline KW - Eu-MWCNTs KW - aptasensor KW - electrochemiluminescence KW - target recycling KW - thrombin SP - 12663 EP - 70 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 7 IS - 23 N2 - A novel electrochemiluminescent (ECL) aptasensor was proposed for the determination of thrombin (TB) using exonuclease-catalyzed target recycling and hybridization chain reaction (HCR) to amplify the signal. The capture probe was immobilized on an Au-GS-modified electrode through a Au-S bond. Subsequently, the hybrid between the capture probe and the complementary thrombin binding aptamer (TBA) was aimed at obtaining double-stranded DNA (dsDNA). The interaction between TB and its aptamer led to the dissociation of dsDNA because TB has a higher affinity to TBA than the complementary strands. In the presence of exonuclease, aptamer was selectively digested and TB could be released for target recycling. Extended dsDNA was formed through HCR of the capture probe and two hairpin DNA strands (NH2-DNA1 and NH2-DNA1). Then, numerous europium multiwalled carbon nanotubes (Eu-MWCNTs) could be introduced through amidation reaction between NH2-terminated DNA strands and carboxyl groups on the Eu-MWCNTs, resulting in an increased ECL signal. The multiple amplification strategies, including the amplification of analyte recycling and HCR, and high ECL efficiency of Eu-MWCNTs lead to a wide linear range (1.0×10(-12)-5.0×10(-9) mol/L) and a low detection limit (0.23 pmol/L). The method was applied to serum sample analysis with satisfactory results. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/26005759/Application_of_Europium_Multiwalled_Carbon_Nanotubes_as_Novel_Luminophores_in_an_Electrochemiluminescent_Aptasensor_for_Thrombin_Using_Multiple_Amplification_Strategies_ L2 - https://doi.org/10.1021/acsami.5b03381 DB - PRIME DP - Unbound Medicine ER -