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Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes.
Int J Environ Res Public Health. 2015 May 20; 12(5):5386-405.IJ

Abstract

Among all classes of nanomaterials, silver nanoparticles (AgNPs) have potentially an important ecotoxicological impact, especially in freshwater environments. Fish are particularly susceptible to the toxic effects of silver ions and, with knowledge gaps regarding the contribution of dissolution and unique particle effects to AgNP toxicity, they represent a group of vulnerable organisms. Using cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) as in vitro test systems, we assessed the cytotoxicity of the representative AgNP, NM-300K, and AgNO3 as an Ag+ ion source. Lack of AgNP interference with the cytotoxicity assays (AlamarBlue, CFDA-AM, NRU assay) and their simultaneous application point to the compatibility and usefulness of such a battery of assays. The RTH-149 and RTL-W1 liver cell lines exhibited similar sensitivity as primary hepatocytes towards AgNP toxicity. Leibovitz's L-15 culture medium composition (high amino acid content) had an important influence on the behaviour and toxicity of AgNPs towards the RTL-W1 cell line. The obtained results demonstrate that, with careful consideration, such an in vitro approach can provide valuable toxicological data to be used in an integrated testing strategy for NM-300K risk assessment.

Authors+Show Affiliations

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, E-38040 Madrid, Spain. connolly.mona@inia.es.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, E-38040 Madrid, Spain. fcruz@inia.es.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, E-38040 Madrid, Spain. quesada.alba@inia.es.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, E-38040 Madrid, Spain. luis.alte@inia.es.Faculty of Vetsuisse, Centre for Fish and Wildlife Health, University of Berne, Länggassstr. 122, Postfach 8466, CH-3001 Bern, Switzerland,. helmut.segner@vetsuisse.unibe.ch.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7.5, E-38040 Madrid, Spain. jmnavas@inia.es.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26006119

Citation

Connolly, Mona, et al. "Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes." International Journal of Environmental Research and Public Health, vol. 12, no. 5, 2015, pp. 5386-405.
Connolly M, Fernandez-Cruz ML, Quesada-Garcia A, et al. Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. Int J Environ Res Public Health. 2015;12(5):5386-405.
Connolly, M., Fernandez-Cruz, M. L., Quesada-Garcia, A., Alte, L., Segner, H., & Navas, J. M. (2015). Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. International Journal of Environmental Research and Public Health, 12(5), 5386-405. https://doi.org/10.3390/ijerph120505386
Connolly M, et al. Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. Int J Environ Res Public Health. 2015 May 20;12(5):5386-405. PubMed PMID: 26006119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. AU - Connolly,Mona, AU - Fernandez-Cruz,Maria-Luisa, AU - Quesada-Garcia,Alba, AU - Alte,Luis, AU - Segner,Helmut, AU - Navas,Jose M, Y1 - 2015/05/20/ PY - 2015/03/26/received PY - 2015/04/30/revised PY - 2015/05/12/accepted PY - 2015/5/27/entrez PY - 2015/5/27/pubmed PY - 2016/5/10/medline KW - AgNPs KW - cytotoxicity KW - in vitro cell lines KW - primary hepatocyte KW - rainbow trout (Oncorhynchus mykiss) SP - 5386 EP - 405 JF - International journal of environmental research and public health JO - Int J Environ Res Public Health VL - 12 IS - 5 N2 - Among all classes of nanomaterials, silver nanoparticles (AgNPs) have potentially an important ecotoxicological impact, especially in freshwater environments. Fish are particularly susceptible to the toxic effects of silver ions and, with knowledge gaps regarding the contribution of dissolution and unique particle effects to AgNP toxicity, they represent a group of vulnerable organisms. Using cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) as in vitro test systems, we assessed the cytotoxicity of the representative AgNP, NM-300K, and AgNO3 as an Ag+ ion source. Lack of AgNP interference with the cytotoxicity assays (AlamarBlue, CFDA-AM, NRU assay) and their simultaneous application point to the compatibility and usefulness of such a battery of assays. The RTH-149 and RTL-W1 liver cell lines exhibited similar sensitivity as primary hepatocytes towards AgNP toxicity. Leibovitz's L-15 culture medium composition (high amino acid content) had an important influence on the behaviour and toxicity of AgNPs towards the RTL-W1 cell line. The obtained results demonstrate that, with careful consideration, such an in vitro approach can provide valuable toxicological data to be used in an integrated testing strategy for NM-300K risk assessment. SN - 1660-4601 UR - https://www.unboundmedicine.com/medline/citation/26006119/Comparative_Cytotoxicity_Study_of_Silver_Nanoparticles__AgNPs__in_a_Variety_of_Rainbow_Trout_Cell_Lines__RTL_W1_RTH_149_RTG_2__and_Primary_Hepatocytes_ DB - PRIME DP - Unbound Medicine ER -