Tags

Type your tag names separated by a space and hit enter

Sonoran propolis and some of its chemical constituents inhibit in vitro growth of Giardia lamblia trophozoites.
Planta Med 2015; 81(9):742-7PM

Abstract

Propolis is a cereus resin with a complex chemical composition that possesses a wide range of biological activities. The aim of this study was to evaluate the in vitro anti-Giardia lamblia activity of Sonoran propolis collected from three different areas of Sonoran Desert in northwestern Mexico (Caborca, Pueblo de Alamos, and Ures) and some of its chemical constituents. Additionally, we also analyzed the seasonal effect on the anti-G. lamblia activity of propolis. G. lamblia trophozoite cultures were treated with different concentrations of Sonoran propolis or chemical compounds during 48 h cell proliferation and cell viability were determined. Ures propolis showed the highest inhibitory activity against G. lamblia (IC50 63.8 ± 7.1 µg/mL) in a dose-dependent manner (Ures > Pueblo de Alamos > Caborca). Season had a significant effect on the in vitro anti-G. lamblia activity of Ures propolis. Summer propolis showed the highest inhibitory effect on the G. lamblia trophozoite growth (IC50 23.8 ± 2.3 µg/mL), followed by propolis collected during winter (IC50 59.2 ± 34.7 µg/mL), spring (IC50 102.5 ± 15.3 µg/mL), and autumn (IC50 125.0 ± 3.1 µg/mL). Caffeic acid phenethyl ester, an Ures propolis exclusive constituent, had the highest growth-inhibitory activity towards G. lamblia [IC50 63.1 ± 0.9 µg/mL (222.1 ± 3.2 µM)]. To our knowledge, this is the first study showing that caffeic acid phenethyl ester possesses antiparasitic activity against G. lamblia. Naringenin [IC50 125.7 ± 20.7 µg/mL (461.8 ± 76.3 µM)], hesperetin [IC50 149.6 ± 24.8 µg/mL (494.9 ± 82.2 µM)], and pinocembrin [IC50 174.4 ± 26.0 µg/mL (680.6 ± 101.7 µM)] showed weak anti-G. lamblia activity. On the other hand, chrysin and rutin did not show significant antiparasitic activity. In conclusion, our results suggest that Sonoran propolis and some of its chemical constituents had inhibitory effects on the in vitro growth of G. lamblia trophozoites.

Authors+Show Affiliations

Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.Department of Chemistry-Biology and Agropecuary Sciences, University of Sonora, Navojoa, Sonora, Mexico.Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo A. C. Hermosillo, Sonora, México.Unidad de Servicios de Apoyo en Resolución Analítica, Universidad Veracruzana, Xalapa, Veracruz, México.Department of Chemistry-Biology, University of Sonora, Hermosillo, Sonora, Mexico.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26008200

Citation

Alday-Provencio, Samuel, et al. "Sonoran Propolis and some of Its Chemical Constituents Inhibit in Vitro Growth of Giardia Lamblia Trophozoites." Planta Medica, vol. 81, no. 9, 2015, pp. 742-7.
Alday-Provencio S, Diaz G, Rascon L, et al. Sonoran propolis and some of its chemical constituents inhibit in vitro growth of Giardia lamblia trophozoites. Planta Med. 2015;81(9):742-7.
Alday-Provencio, S., Diaz, G., Rascon, L., Quintero, J., Alday, E., Robles-Zepeda, R., ... Velazquez, C. (2015). Sonoran propolis and some of its chemical constituents inhibit in vitro growth of Giardia lamblia trophozoites. Planta Medica, 81(9), pp. 742-7. doi:10.1055/s-0035-1545982.
Alday-Provencio S, et al. Sonoran Propolis and some of Its Chemical Constituents Inhibit in Vitro Growth of Giardia Lamblia Trophozoites. Planta Med. 2015;81(9):742-7. PubMed PMID: 26008200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sonoran propolis and some of its chemical constituents inhibit in vitro growth of Giardia lamblia trophozoites. AU - Alday-Provencio,Samuel, AU - Diaz,Gabriela, AU - Rascon,Lucila, AU - Quintero,Jael, AU - Alday,Efrain, AU - Robles-Zepeda,Ramón, AU - Garibay-Escobar,Adriana, AU - Astiazaran,Humberto, AU - Hernandez,Javier, AU - Velazquez,Carlos, Y1 - 2015/05/22/ PY - 2015/5/27/entrez PY - 2015/5/27/pubmed PY - 2016/1/12/medline SP - 742 EP - 7 JF - Planta medica JO - Planta Med. VL - 81 IS - 9 N2 - Propolis is a cereus resin with a complex chemical composition that possesses a wide range of biological activities. The aim of this study was to evaluate the in vitro anti-Giardia lamblia activity of Sonoran propolis collected from three different areas of Sonoran Desert in northwestern Mexico (Caborca, Pueblo de Alamos, and Ures) and some of its chemical constituents. Additionally, we also analyzed the seasonal effect on the anti-G. lamblia activity of propolis. G. lamblia trophozoite cultures were treated with different concentrations of Sonoran propolis or chemical compounds during 48 h cell proliferation and cell viability were determined. Ures propolis showed the highest inhibitory activity against G. lamblia (IC50 63.8 ± 7.1 µg/mL) in a dose-dependent manner (Ures > Pueblo de Alamos > Caborca). Season had a significant effect on the in vitro anti-G. lamblia activity of Ures propolis. Summer propolis showed the highest inhibitory effect on the G. lamblia trophozoite growth (IC50 23.8 ± 2.3 µg/mL), followed by propolis collected during winter (IC50 59.2 ± 34.7 µg/mL), spring (IC50 102.5 ± 15.3 µg/mL), and autumn (IC50 125.0 ± 3.1 µg/mL). Caffeic acid phenethyl ester, an Ures propolis exclusive constituent, had the highest growth-inhibitory activity towards G. lamblia [IC50 63.1 ± 0.9 µg/mL (222.1 ± 3.2 µM)]. To our knowledge, this is the first study showing that caffeic acid phenethyl ester possesses antiparasitic activity against G. lamblia. Naringenin [IC50 125.7 ± 20.7 µg/mL (461.8 ± 76.3 µM)], hesperetin [IC50 149.6 ± 24.8 µg/mL (494.9 ± 82.2 µM)], and pinocembrin [IC50 174.4 ± 26.0 µg/mL (680.6 ± 101.7 µM)] showed weak anti-G. lamblia activity. On the other hand, chrysin and rutin did not show significant antiparasitic activity. In conclusion, our results suggest that Sonoran propolis and some of its chemical constituents had inhibitory effects on the in vitro growth of G. lamblia trophozoites. SN - 1439-0221 UR - https://www.unboundmedicine.com/medline/citation/26008200/Sonoran_propolis_and_some_of_its_chemical_constituents_inhibit_in_vitro_growth_of_Giardia_lamblia_trophozoites_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-0035-1545982 DB - PRIME DP - Unbound Medicine ER -