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Primary afferent neurons containing calcitonin gene-related peptide but not substance P in forepaw skin, dorsal root ganglia, and spinal cord of mice.
J Comp Neurol. 2015 Dec 01; 523(17):2555-69.JC

Abstract

In mice dorsal root ganglia (DRG), some neurons express calcitonin gene-related peptide (CGRP) without substance P (SP; CGRP(+) SP(-)). The projections and functions of these neurons are unknown. Therefore, we combined in vitro axonal tracing with multiple-labeling immunohistochemistry to neurochemically define these neurons and characterize their peripheral and central projections. Cervical spinal cord, DRG, and forepaw skin were removed from C57Bl/6 mice and multiple-labeled for CGRP, SP, and either marker for the sensory neuron subpopulations transient receptor potential vanilloid type 1 (TRPV1), neurofilament 200 (NF200), or vesicular glutamate transporter 2 (VGluT1). To determine central projections of CGRP(+) SP(-) neurons, Neurobiotin (NB) was applied to the C7 ventral ramus and visualized in DRG and spinal cord sections colabeled for CGRP and SP. Half (50%) of the CGRP-immunoreactive DRG neurons lacked detectable SP and had a mean soma size of 473 ± 14 μm(2) (n = 5); 89% of the CGRP(+) SP(-) neurons expressed NF200 (n = 5), but only 32% expressed TRPV1 (n = 5). Cutaneous CGRP(+) SP(-) fibers were numerous within dermal papillae and around hair shafts (n = 4). CGRP(+) SP(-) boutons were prevalent in lateral lamina I and in lamina IV/V of the dorsal horn (n = 5). NB predominantly labeled fibers penetrating lamina IV/V, 6 ± 3% contained CGRP (n = 5), and 21 ± 2% contained VGluT1 (n = 3). CGRP(+) SP(-) afferent neurons are likely to be non-nociceptive. Their soma size, neurochemical profile, and peripheral and central targets suggest that CGRP(+) SP(-) neurons are polymodal mechanoceptors.

Authors+Show Affiliations

Department of Anatomy and Histology, and Centre for Neuroscience, Flinders University, Adelaide, South Australia, 5001, Australia.Department of Anatomy and Histology, and Centre for Neuroscience, Flinders University, Adelaide, South Australia, 5001, Australia.Department of Anatomy and Histology, and Centre for Neuroscience, Flinders University, Adelaide, South Australia, 5001, Australia.Department of Anatomy and Histology, and Centre for Neuroscience, Flinders University, Adelaide, South Australia, 5001, Australia.Department of Anatomy and Histology, and Centre for Neuroscience, Flinders University, Adelaide, South Australia, 5001, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26010480

Citation

Kestell, Garreth R., et al. "Primary Afferent Neurons Containing Calcitonin Gene-related Peptide but Not Substance P in Forepaw Skin, Dorsal Root Ganglia, and Spinal Cord of Mice." The Journal of Comparative Neurology, vol. 523, no. 17, 2015, pp. 2555-69.
Kestell GR, Anderson RL, Clarke JN, et al. Primary afferent neurons containing calcitonin gene-related peptide but not substance P in forepaw skin, dorsal root ganglia, and spinal cord of mice. J Comp Neurol. 2015;523(17):2555-69.
Kestell, G. R., Anderson, R. L., Clarke, J. N., Haberberger, R. V., & Gibbins, I. L. (2015). Primary afferent neurons containing calcitonin gene-related peptide but not substance P in forepaw skin, dorsal root ganglia, and spinal cord of mice. The Journal of Comparative Neurology, 523(17), 2555-69. https://doi.org/10.1002/cne.23804
Kestell GR, et al. Primary Afferent Neurons Containing Calcitonin Gene-related Peptide but Not Substance P in Forepaw Skin, Dorsal Root Ganglia, and Spinal Cord of Mice. J Comp Neurol. 2015 Dec 1;523(17):2555-69. PubMed PMID: 26010480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Primary afferent neurons containing calcitonin gene-related peptide but not substance P in forepaw skin, dorsal root ganglia, and spinal cord of mice. AU - Kestell,Garreth R, AU - Anderson,Rebecca L, AU - Clarke,Jennifer N, AU - Haberberger,Rainer V, AU - Gibbins,Ian L, Y1 - 2015/06/11/ PY - 2014/08/18/received PY - 2015/04/20/revised PY - 2015/04/29/accepted PY - 2015/5/27/entrez PY - 2015/5/27/pubmed PY - 2016/7/7/medline KW - AB_2313819 KW - AB_2314983 KW - AB_2315370 KW - AB_2315546 KW - AB_477257 KW - CGRP KW - DRG KW - NF200 KW - RRIDs: AB_572266 KW - TRPV1 KW - axonal tracing KW - nociception SP - 2555 EP - 69 JF - The Journal of comparative neurology JO - J Comp Neurol VL - 523 IS - 17 N2 - In mice dorsal root ganglia (DRG), some neurons express calcitonin gene-related peptide (CGRP) without substance P (SP; CGRP(+) SP(-)). The projections and functions of these neurons are unknown. Therefore, we combined in vitro axonal tracing with multiple-labeling immunohistochemistry to neurochemically define these neurons and characterize their peripheral and central projections. Cervical spinal cord, DRG, and forepaw skin were removed from C57Bl/6 mice and multiple-labeled for CGRP, SP, and either marker for the sensory neuron subpopulations transient receptor potential vanilloid type 1 (TRPV1), neurofilament 200 (NF200), or vesicular glutamate transporter 2 (VGluT1). To determine central projections of CGRP(+) SP(-) neurons, Neurobiotin (NB) was applied to the C7 ventral ramus and visualized in DRG and spinal cord sections colabeled for CGRP and SP. Half (50%) of the CGRP-immunoreactive DRG neurons lacked detectable SP and had a mean soma size of 473 ± 14 μm(2) (n = 5); 89% of the CGRP(+) SP(-) neurons expressed NF200 (n = 5), but only 32% expressed TRPV1 (n = 5). Cutaneous CGRP(+) SP(-) fibers were numerous within dermal papillae and around hair shafts (n = 4). CGRP(+) SP(-) boutons were prevalent in lateral lamina I and in lamina IV/V of the dorsal horn (n = 5). NB predominantly labeled fibers penetrating lamina IV/V, 6 ± 3% contained CGRP (n = 5), and 21 ± 2% contained VGluT1 (n = 3). CGRP(+) SP(-) afferent neurons are likely to be non-nociceptive. Their soma size, neurochemical profile, and peripheral and central targets suggest that CGRP(+) SP(-) neurons are polymodal mechanoceptors. SN - 1096-9861 UR - https://www.unboundmedicine.com/medline/citation/26010480/Primary_afferent_neurons_containing_calcitonin_gene_related_peptide_but_not_substance_P_in_forepaw_skin_dorsal_root_ganglia_and_spinal_cord_of_mice_ L2 - https://doi.org/10.1002/cne.23804 DB - PRIME DP - Unbound Medicine ER -