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Fresh water fish, Channa punctatus, as a model for pendimethalin genotoxicity testing: A new approach toward aquatic environmental contaminants.
Environ Toxicol. 2016 Nov; 31(11):1520-1529.ET

Abstract

Pendimethalin (PND) is one of the common herbicides used worldwide. Fresh water fish, Channa punctatus, was exposed to PND in aquaria wherein its LC50 value was recorded to be 3.6 mg/L. Three sublethal (SL) concentrations, namely, 0.9, 1.8, and 2.7 mg/L were selected for the evaluation of genotoxicity and oxidative stress generated in the fish. In vivo comet assay was carried out in the blood, liver, and gill cells after exposing the fish to aforesaid SL concentrations of PND for 24, 48, 72, and 96 h. The results of the comet assay demonstrated the genotoxicity of PND in all the three tissues. Induction of oxidative stress in the gill cells was affirmed by the increased lipid peroxidation (LPO) and decreased levels of reduced glutathione, superoxide dismutase, and catalase. Frequencies of erythrocytic nuclear abnormalities (ENA) and micronuclei (MN) were also used to assess the genotoxic potential of PND on C. punctatus. MN frequency did not show any enhancement after PND exposure, but the frequency of ENA such as kidney-shaped nuclei, segmented nuclei and lobed nuclei, showed a significant increase after 24-96 h. Thus, ENA seems to be a better biomarker than MN for PND induced genotoxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1520-1529, 2016.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India.Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, 202002, India. masoodamua@rediffmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26011309

Citation

Ahmad, Irshad, and Masood Ahmad. "Fresh Water Fish, Channa Punctatus, as a Model for Pendimethalin Genotoxicity Testing: a New Approach Toward Aquatic Environmental Contaminants." Environmental Toxicology, vol. 31, no. 11, 2016, pp. 1520-1529.
Ahmad I, Ahmad M. Fresh water fish, Channa punctatus, as a model for pendimethalin genotoxicity testing: A new approach toward aquatic environmental contaminants. Environ Toxicol. 2016;31(11):1520-1529.
Ahmad, I., & Ahmad, M. (2016). Fresh water fish, Channa punctatus, as a model for pendimethalin genotoxicity testing: A new approach toward aquatic environmental contaminants. Environmental Toxicology, 31(11), 1520-1529. https://doi.org/10.1002/tox.22156
Ahmad I, Ahmad M. Fresh Water Fish, Channa Punctatus, as a Model for Pendimethalin Genotoxicity Testing: a New Approach Toward Aquatic Environmental Contaminants. Environ Toxicol. 2016;31(11):1520-1529. PubMed PMID: 26011309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fresh water fish, Channa punctatus, as a model for pendimethalin genotoxicity testing: A new approach toward aquatic environmental contaminants. AU - Ahmad,Irshad, AU - Ahmad,Masood, Y1 - 2015/05/26/ PY - 2014/08/06/received PY - 2015/04/25/revised PY - 2015/05/10/accepted PY - 2016/10/19/pubmed PY - 2017/3/4/medline PY - 2015/5/27/entrez KW - comet assay KW - erythrocytic nuclear abnormalities KW - genotoxicity KW - oxidative stress KW - pendimethalin SP - 1520 EP - 1529 JF - Environmental toxicology JO - Environ Toxicol VL - 31 IS - 11 N2 - Pendimethalin (PND) is one of the common herbicides used worldwide. Fresh water fish, Channa punctatus, was exposed to PND in aquaria wherein its LC50 value was recorded to be 3.6 mg/L. Three sublethal (SL) concentrations, namely, 0.9, 1.8, and 2.7 mg/L were selected for the evaluation of genotoxicity and oxidative stress generated in the fish. In vivo comet assay was carried out in the blood, liver, and gill cells after exposing the fish to aforesaid SL concentrations of PND for 24, 48, 72, and 96 h. The results of the comet assay demonstrated the genotoxicity of PND in all the three tissues. Induction of oxidative stress in the gill cells was affirmed by the increased lipid peroxidation (LPO) and decreased levels of reduced glutathione, superoxide dismutase, and catalase. Frequencies of erythrocytic nuclear abnormalities (ENA) and micronuclei (MN) were also used to assess the genotoxic potential of PND on C. punctatus. MN frequency did not show any enhancement after PND exposure, but the frequency of ENA such as kidney-shaped nuclei, segmented nuclei and lobed nuclei, showed a significant increase after 24-96 h. Thus, ENA seems to be a better biomarker than MN for PND induced genotoxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1520-1529, 2016. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/26011309/Fresh_water_fish_Channa_punctatus_as_a_model_for_pendimethalin_genotoxicity_testing:_A_new_approach_toward_aquatic_environmental_contaminants_ L2 - https://doi.org/10.1002/tox.22156 DB - PRIME DP - Unbound Medicine ER -