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Vitamin D levels and vitamin D receptor gene polymorphisms in asthmatic children: a case-control study.
Pediatr Allergy Immunol. 2015 Sep; 26(6):545-50.PA

Abstract

BACKGROUND

Vitamin D deficiency and single nucleotide polymorphisms (SNP) in the gene encoding vitamin D receptor (VDR) have been associated with asthma.

OBJECTIVE

To compare 25-hydroxyvitamin D (25OHD) levels and the frequency of 3 SNPs in the VDR gene between asthmatic and healthy children.

METHODS

In persistent asthmatic and healthy control children, the 25OHD levels were measured using radioimmunoassay and SNPs (FokI, ApaI, and TaqI) were analyzed by a PCR-RFLP assay. Relevant medical history was collected.

RESULTS

About 75 asthmatic (median age: 9.1 years) and 227 healthy children (10.3 years) were studied. In the whole population, the proportion of sufficient, insufficient, and deficient levels of 25OHD were 14.9%, 44%, and 41.1%, respectively. 25OHD sufficiency status was similar in asthmatic and healthy children (p = 0.57). However, the proportion of 25OHD sufficient levels among asthmatics according to the Global Initiative for Asthma treatment steps 2, 3, and 4 was significantly different (8.6%, 16.6%, and 43.7%, respectively, p = 0.046). All patients on step 4 of the treatment (16/16) were heterozygous for the C allele (FokI VDR SNP). There was a lower presence of the C allele among asthmatics in step 2 (30/33), step 3 (16/24), and controls (45/50), p = 0.007, but this significance did not persist after logistic regression. No significant differences in ApaI and TaqI were found.

CONCLUSIONS

We found a possible association of vitamin D sufficiency status and FokI C allele with higher requirement of therapy to reach asthma control, suggesting that it may be involved in treatment response. Variations in VDR might also play a role in the 25OHD levels.

Authors+Show Affiliations

Pulmonology Section, Division of Pediatric, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Endocrionology Section, Division of Pediatric, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Division of Public Health, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Laboratorio de Virología Molecular, Instituto Milenio de Inmunología e Inmunoterapia, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.Pulmonology Section, Division of Pediatric, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. Division of Public Health, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26011658

Citation

Einisman, Helly, et al. "Vitamin D Levels and Vitamin D Receptor Gene Polymorphisms in Asthmatic Children: a Case-control Study." Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, vol. 26, no. 6, 2015, pp. 545-50.
Einisman H, Reyes ML, Angulo J, et al. Vitamin D levels and vitamin D receptor gene polymorphisms in asthmatic children: a case-control study. Pediatr Allergy Immunol. 2015;26(6):545-50.
Einisman, H., Reyes, M. L., Angulo, J., Cerda, J., López-Lastra, M., & Castro-Rodriguez, J. A. (2015). Vitamin D levels and vitamin D receptor gene polymorphisms in asthmatic children: a case-control study. Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, 26(6), 545-50. https://doi.org/10.1111/pai.12409
Einisman H, et al. Vitamin D Levels and Vitamin D Receptor Gene Polymorphisms in Asthmatic Children: a Case-control Study. Pediatr Allergy Immunol. 2015;26(6):545-50. PubMed PMID: 26011658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitamin D levels and vitamin D receptor gene polymorphisms in asthmatic children: a case-control study. AU - Einisman,Helly, AU - Reyes,María Loreto, AU - Angulo,Jenniffer, AU - Cerda,Jaime, AU - López-Lastra,Marcelo, AU - Castro-Rodriguez,Jose A, Y1 - 2015/06/17/ PY - 2015/05/14/accepted PY - 2015/5/27/entrez PY - 2015/5/27/pubmed PY - 2016/7/1/medline KW - ApaI KW - FokI KW - Global Initiative for Asthma KW - TaqI KW - asthma KW - children KW - single nucleotide polymorphisms KW - treatment KW - vitamin D KW - vitamin D receptor SP - 545 EP - 50 JF - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology JO - Pediatr Allergy Immunol VL - 26 IS - 6 N2 - BACKGROUND: Vitamin D deficiency and single nucleotide polymorphisms (SNP) in the gene encoding vitamin D receptor (VDR) have been associated with asthma. OBJECTIVE: To compare 25-hydroxyvitamin D (25OHD) levels and the frequency of 3 SNPs in the VDR gene between asthmatic and healthy children. METHODS: In persistent asthmatic and healthy control children, the 25OHD levels were measured using radioimmunoassay and SNPs (FokI, ApaI, and TaqI) were analyzed by a PCR-RFLP assay. Relevant medical history was collected. RESULTS: About 75 asthmatic (median age: 9.1 years) and 227 healthy children (10.3 years) were studied. In the whole population, the proportion of sufficient, insufficient, and deficient levels of 25OHD were 14.9%, 44%, and 41.1%, respectively. 25OHD sufficiency status was similar in asthmatic and healthy children (p = 0.57). However, the proportion of 25OHD sufficient levels among asthmatics according to the Global Initiative for Asthma treatment steps 2, 3, and 4 was significantly different (8.6%, 16.6%, and 43.7%, respectively, p = 0.046). All patients on step 4 of the treatment (16/16) were heterozygous for the C allele (FokI VDR SNP). There was a lower presence of the C allele among asthmatics in step 2 (30/33), step 3 (16/24), and controls (45/50), p = 0.007, but this significance did not persist after logistic regression. No significant differences in ApaI and TaqI were found. CONCLUSIONS: We found a possible association of vitamin D sufficiency status and FokI C allele with higher requirement of therapy to reach asthma control, suggesting that it may be involved in treatment response. Variations in VDR might also play a role in the 25OHD levels. SN - 1399-3038 UR - https://www.unboundmedicine.com/medline/citation/26011658/Vitamin_D_levels_and_vitamin_D_receptor_gene_polymorphisms_in_asthmatic_children:_a_case_control_study_ L2 - https://doi.org/10.1111/pai.12409 DB - PRIME DP - Unbound Medicine ER -