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Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to a comparative pharmacokinetic study.
Biomed Chromatogr. 2016 Feb; 30(2):97-104.BC

Abstract

A simple, reliable and rapid ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of four secoiridoid (gentiopicroside, swertiamarin, sweroside) and iridoid glycosides (loganic acid), the bio-active ingredients in rat plasma. After liquid-liquid extraction, chromatographic separation was accomplished on a Shim-pack XR-ODS column with a mobile phase consisting of methanol and 0.1% formic acid in water. A triple quadrupole tandem mass spectrometry equipped with an electrospray ionization source was used as detector operating both in positive and negative ionization mode and operated by multiple-reaction monitoring scanning. The lower limits of quantitation were 0.25-30 ng/mL for all the analytes. Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria (±15%). The mean extraction recoveries of analytes and internal standard (amygdalin) from rat plasma were all >71.4%. The validated method was successfully applied to a comparative pharmacokinetic study of four analytes in rat plasma between normal and arthritic rats after oral administration of Huo Luo Xiao Ling Dan and Gentiana macrophylla extract, respectively. Results showed significant differences in pharmacokinetic properties of the analytes among the different groups.

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Publisher Full Text (DOI)

Authors+Show Affiliations

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

Mailman Research Center, McLean Hospital, Harvard Medical School, Boston, MA, United States.

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China.

MeSH

Administration, OralAnimalsArthritis, ExperimentalDrugs, Chinese HerbalIridoid GlycosidesLinear ModelsMaleRatsRats, Sprague-DawleyReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26014753

Citation

Wu, Yun, et al. "Simultaneous Determination of Four Secoiridoid and Iridoid Glycosides in Rat Plasma By Ultra Performance Liquid Chromatography-tandem Mass Spectrometry and Its Application to a Comparative Pharmacokinetic Study." Biomedical Chromatography : BMC, vol. 30, no. 2, 2016, pp. 97-104.

Wu Y, Ai Y, Wang F, et al. Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to a comparative pharmacokinetic study. Biomed Chromatogr. 2016;30(2):97-104.

Wu, Y., Ai, Y., Wang, F., Ma, W., Bian, Q., Lee, D. Y., & Dai, R. (2016). Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to a comparative pharmacokinetic study. Biomedical Chromatography : BMC, 30(2), 97-104. https://doi.org/10.1002/bmc.3519

Wu Y, et al. Simultaneous Determination of Four Secoiridoid and Iridoid Glycosides in Rat Plasma By Ultra Performance Liquid Chromatography-tandem Mass Spectrometry and Its Application to a Comparative Pharmacokinetic Study. Biomed Chromatogr. 2016;30(2):97-104. PubMed PMID: 26014753.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Simultaneous determination of four secoiridoid and iridoid glycosides in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry and its application to a comparative pharmacokinetic study. AU - Wu,Yun, AU - Ai,Yu, AU - Wang,Fenrong, AU - Ma,Wen, AU - Bian,Qiaoxia, AU - Lee,David Y-W, AU - Dai,Ronghua, Y1 - 2015/07/06/ PY - 2015/02/06/received PY - 2015/04/21/revised PY - 2015/05/18/accepted PY - 2015/5/28/entrez PY - 2015/5/28/pubmed PY - 2016/8/20/medline KW - Huo Luo Xiao Ling Dan KW - UPLC-MS/MS KW - arthritis KW - comparative pharmacokinetics KW - rat plasma SP - 97 EP - 104 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 30 IS - 2 N2 - A simple, reliable and rapid ultra-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous quantification of four secoiridoid (gentiopicroside, swertiamarin, sweroside) and iridoid glycosides (loganic acid), the bio-active ingredients in rat plasma. After liquid-liquid extraction, chromatographic separation was accomplished on a Shim-pack XR-ODS column with a mobile phase consisting of methanol and 0.1% formic acid in water. A triple quadrupole tandem mass spectrometry equipped with an electrospray ionization source was used as detector operating both in positive and negative ionization mode and operated by multiple-reaction monitoring scanning. The lower limits of quantitation were 0.25-30 ng/mL for all the analytes. Both intra-day and inter-day precision and accuracy of analytes were well within acceptance criteria (±15%). The mean extraction recoveries of analytes and internal standard (amygdalin) from rat plasma were all >71.4%. The validated method was successfully applied to a comparative pharmacokinetic study of four analytes in rat plasma between normal and arthritic rats after oral administration of Huo Luo Xiao Ling Dan and Gentiana macrophylla extract, respectively. Results showed significant differences in pharmacokinetic properties of the analytes among the different groups. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/26014753/Simultaneous_determination_of_four_secoiridoid_and_iridoid_glycosides_in_rat_plasma_by_ultra_performance_liquid_chromatography_tandem_mass_spectrometry_and_its_application_to_a_comparative_pharmacokinetic_study_ DB - PRIME DP - Unbound Medicine ER -