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A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184.
Plasmid 2015; 81:21-6P

Abstract

The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.

Authors+Show Affiliations

Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA.Department of Microbiology, University of Illinois, B103 Chemical and Life Sciences Laboratory, 601S. Goodwin Ave., Urbana, IL 61801, USA; Department of Biochemistry, University of Illinois, 419 Roger Adams Laboratory, 600 South Mathews Ave., Urbana, IL 61801, USA. Electronic address: j-cronan@life.uiuc.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

26021570

Citation

Chakravartty, Vandana, and John E. Cronan. "A Series of Medium and High Copy Number Arabinose-inducible Escherichia Coli Expression Vectors Compatible With pBR322 and PACYC184." Plasmid, vol. 81, 2015, pp. 21-6.
Chakravartty V, Cronan JE. A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184. Plasmid. 2015;81:21-6.
Chakravartty, V., & Cronan, J. E. (2015). A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184. Plasmid, 81, pp. 21-6. doi:10.1016/j.plasmid.2015.03.001.
Chakravartty V, Cronan JE. A Series of Medium and High Copy Number Arabinose-inducible Escherichia Coli Expression Vectors Compatible With pBR322 and PACYC184. Plasmid. 2015;81:21-6. PubMed PMID: 26021570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184. AU - Chakravartty,Vandana, AU - Cronan,John E, Y1 - 2015/05/27/ PY - 2014/12/22/received PY - 2015/02/24/revised PY - 2015/03/03/accepted PY - 2015/5/30/entrez PY - 2015/5/30/pubmed PY - 2016/7/28/medline KW - Copy number KW - Expression levels KW - Plasmid Compatibility KW - RSF1030 KW - pBAD24 KW - pBR322 SP - 21 EP - 6 JF - Plasmid JO - Plasmid VL - 81 N2 - The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available. SN - 1095-9890 UR - https://www.unboundmedicine.com/medline/citation/26021570/A_series_of_medium_and_high_copy_number_arabinose_inducible_Escherichia_coli_expression_vectors_compatible_with_pBR322_and_pACYC184_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(15)00019-0 DB - PRIME DP - Unbound Medicine ER -