Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle.
C R Biol. 2015 Jul; 338(7):443-50.CR

Abstract

The Colorado potato beetle (Leptinotarsa decemlineata Say, CPB) is a fatal pest, which is a quarantine pest in China. The CPB has now invaded the Xinjiang Uygur Autonomous Region and is constantly spreading eastward in China. In this study, we developed transgenic potato plants expressing cry3A gene. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the cry3A gene expressed in leaves, stems and roots of the transgenic plants under the control of CaMV 35S promoter, while they expressed only in leaves and stems under the control of potato leaf and stem-specific promoter ST-LS1. The mortality of the larvae was higher (28% and 36%) on the transgenic plant line 35S1 on the 3rd and 4th days, and on ST3 (48%) on the 5th day after inoculation with instar larvae. Insect biomass accumulation on the foliage of the transgenic plant lines 35S1, 35S2 and ST3 was significantly lower (0.42%, 0.43% and 0.42%). Foliage consumption was lowest on transgenic lines 35S8 and ST2 among all plant foliage (7.47 mg/larvae/day and 12.46 mg/larvae/day). The different transgenic plant foliages had varied inhibition to larval growth. The survivors on the transgenic lines obviously were smaller than their original size and extremely weak. The transgenic potato plants with CPB resistance could be used to develop germplasms or varieties for controlling CPB damage and halting its spread in China.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Mi X

Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.

State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Zhang N

Wang D

Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.

MeSH

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsChinaColeopteraEndotoxinsFeeding BehaviorHemolysin ProteinsLarvaPest ControlPlant LeavesPlant RootsPlant StemsPlants, Genetically ModifiedPromoter Regions, GeneticReal-Time Polymerase Chain ReactionSolanum tuberosumTime Factors

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26025753

Citation

Mi, Xiaoxiao, et al. "Transgenic Potato Plants Expressing cry3A Gene Confer Resistance to Colorado Potato Beetle." Comptes Rendus Biologies, vol. 338, no. 7, 2015, pp. 443-50.

Mi X, Ji X, Yang J, et al. Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle. C R Biol. 2015;338(7):443-50.

Mi, X., Ji, X., Yang, J., Liang, L., Si, H., Wu, J., Zhang, N., & Wang, D. (2015). Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle. Comptes Rendus Biologies, 338(7), 443-50. https://doi.org/10.1016/j.crvi.2015.04.005

Mi X, et al. Transgenic Potato Plants Expressing cry3A Gene Confer Resistance to Colorado Potato Beetle. C R Biol. 2015;338(7):443-50. PubMed PMID: 26025753.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle. AU - Mi,Xiaoxiao, AU - Ji,Xiangzhuo, AU - Yang,Jiangwei, AU - Liang,Lina, AU - Si,Huaijun, AU - Wu,Jiahe, AU - Zhang,Ning, AU - Wang,Di, Y1 - 2015/05/27/ PY - 2015/02/17/received PY - 2015/04/19/revised PY - 2015/04/20/accepted PY - 2015/5/31/entrez PY - 2015/5/31/pubmed PY - 2016/3/12/medline KW - Colorado potato beetle (CPB) KW - Cry3A KW - Insect resistance KW - Transgenic potato SP - 443 EP - 50 JF - Comptes rendus biologies JO - C R Biol VL - 338 IS - 7 N2 - The Colorado potato beetle (Leptinotarsa decemlineata Say, CPB) is a fatal pest, which is a quarantine pest in China. The CPB has now invaded the Xinjiang Uygur Autonomous Region and is constantly spreading eastward in China. In this study, we developed transgenic potato plants expressing cry3A gene. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the cry3A gene expressed in leaves, stems and roots of the transgenic plants under the control of CaMV 35S promoter, while they expressed only in leaves and stems under the control of potato leaf and stem-specific promoter ST-LS1. The mortality of the larvae was higher (28% and 36%) on the transgenic plant line 35S1 on the 3rd and 4th days, and on ST3 (48%) on the 5th day after inoculation with instar larvae. Insect biomass accumulation on the foliage of the transgenic plant lines 35S1, 35S2 and ST3 was significantly lower (0.42%, 0.43% and 0.42%). Foliage consumption was lowest on transgenic lines 35S8 and ST2 among all plant foliage (7.47 mg/larvae/day and 12.46 mg/larvae/day). The different transgenic plant foliages had varied inhibition to larval growth. The survivors on the transgenic lines obviously were smaller than their original size and extremely weak. The transgenic potato plants with CPB resistance could be used to develop germplasms or varieties for controlling CPB damage and halting its spread in China. SN - 1768-3238 UR - https://www.unboundmedicine.com/medline/citation/26025753/Transgenic_potato_plants_expressing_cry3A_gene_confer_resistance_to_Colorado_potato_beetle_ DB - PRIME DP - Unbound Medicine ER -

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Transgenic potato plants expressing cry3A gene confer resistance to Colorado potato beetle.C R Biol. 2015 Jul; 338(7):443-50.CR