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Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina.
Rev Argent Microbiol 2015 Apr-Jun; 47(2):95-102RA

Abstract

The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7%) healthy and 225 (36.3%) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1% isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.

Authors+Show Affiliations

Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina. Electronic address: nayapicco@yahoo.com.ar.Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.Grupo Sanidad Animal, Estación Experimental Agrícola INTA Balcarce, Buenos Aires, Argentina.Grupo Sanidad Animal, Estación Experimental Agrícola INTA Balcarce, Buenos Aires, Argentina.Departamento de Anatomía Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26026231

Citation

Picco, Natalia Y., et al. "Molecular Screening of Pathogenic Escherichia Coli Strains Isolated From Dairy Neonatal Calves in Cordoba Province, Argentina." Revista Argentina De Microbiologia, vol. 47, no. 2, 2015, pp. 95-102.
Picco NY, Alustiza FE, Bellingeri RV, et al. Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina. Rev Argent Microbiol. 2015;47(2):95-102.
Picco, N. Y., Alustiza, F. E., Bellingeri, R. V., Grosso, M. C., Motta, C. E., Larriestra, A. J., ... Vivas, A. B. (2015). Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina. Revista Argentina De Microbiologia, 47(2), pp. 95-102. doi:10.1016/j.ram.2015.01.006.
Picco NY, et al. Molecular Screening of Pathogenic Escherichia Coli Strains Isolated From Dairy Neonatal Calves in Cordoba Province, Argentina. Rev Argent Microbiol. 2015;47(2):95-102. PubMed PMID: 26026231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular screening of pathogenic Escherichia coli strains isolated from dairy neonatal calves in Cordoba province, Argentina. AU - Picco,Natalia Y, AU - Alustiza,Fabrisio E, AU - Bellingeri,Romina V, AU - Grosso,María C, AU - Motta,Carlos E, AU - Larriestra,Alejandro J, AU - Vissio,Claudina, AU - Tiranti,Karina I, AU - Terzolo,Horacio R, AU - Moreira,Ana R, AU - Vivas,Adriana B, Y1 - 2015/05/27/ PY - 2014/07/25/received PY - 2015/01/28/revised PY - 2015/01/31/accepted PY - 2015/6/1/entrez PY - 2015/6/1/pubmed PY - 2015/10/21/medline KW - Argentina KW - Calves KW - Diarrea KW - Diarrhea KW - Escherichia coli KW - Genes de virulencia KW - PCR KW - Terneros KW - Virulence genes SP - 95 EP - 102 JF - Revista Argentina de microbiologia JO - Rev. Argent. Microbiol. VL - 47 IS - 2 N2 - The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7%) healthy and 225 (36.3%) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1% isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/26026231/Molecular_screening_of_pathogenic_Escherichia_coli_strains_isolated_from_dairy_neonatal_calves_in_Cordoba_province_Argentina_ L2 - http://zl.elsevier.es/es/linksolver/ft/pii/S0325-7541(15)00031-0 DB - PRIME DP - Unbound Medicine ER -