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Copper-based nanoparticles induce high toxicity in leukemic HL60 cells.
Toxicol In Vitro. 2015 Oct; 29(7):1711-9.TV

Abstract

From the increasing societal use of nanoparticles (NPs) follows the necessity to understand their potential toxic effects. This requires an in-depth understanding of the relationship between their physicochemical properties and their toxicological behavior. The aim of the present work was to study the toxicity of Cu and CuO NPs toward the leukemic cell line HL60. The toxicity was explored in terms of mitochondrial damage, DNA damage, oxidative DNA damage, cell death and reactive oxygen species (ROS) formation. Particle characteristics and copper release were specifically investigated in order to gain an improved understanding of prevailing toxic mechanisms. The Cu NPs revealed higher toxicity compared with both CuO NPs and dissolved copper (CuCl2), as well as a more rapid copper release compared with CuO NPs. Mitochondrial damage was induced by Cu NPs already after 2 h exposure. Cu NPs induced oxidation at high levels in an acellular ROS assay, and a small increase of intracellular ROS was observed. The increase of DNA damage was limited. CuO NPs did not induce any mitochondrial damage up to 6 h of exposure. No acellular ROS was induced by the CuO NPs, and the levels of intracellular ROS and DNA damage were limited after 2 h exposure. Necrosis was the main type of cell death observed after 18 h exposure to CuO NP and dissolved copper.

Authors+Show Affiliations

Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83 Huddinge, Stockholm, Sweden.KTH Royal Institute of Technology, Division of Surface and Corrosion Science, School of Chemical Science and Engineering, SE-100 44 Stockholm, Sweden.KTH Royal Institute of Technology, Division of Surface and Corrosion Science, School of Chemical Science and Engineering, SE-100 44 Stockholm, Sweden.Department of Laboratory Medicine, Karolinska Institutet, SE-141 83 Huddinge, Stockholm, Sweden.Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83 Huddinge, Stockholm, Sweden. Electronic address: lennart.moller@ki.se.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26028147

Citation

Rodhe, Ylva, et al. "Copper-based Nanoparticles Induce High Toxicity in Leukemic HL60 Cells." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 29, no. 7, 2015, pp. 1711-9.
Rodhe Y, Skoglund S, Odnevall Wallinder I, et al. Copper-based nanoparticles induce high toxicity in leukemic HL60 cells. Toxicol In Vitro. 2015;29(7):1711-9.
Rodhe, Y., Skoglund, S., Odnevall Wallinder, I., Potácová, Z., & Möller, L. (2015). Copper-based nanoparticles induce high toxicity in leukemic HL60 cells. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 29(7), 1711-9. https://doi.org/10.1016/j.tiv.2015.05.020
Rodhe Y, et al. Copper-based Nanoparticles Induce High Toxicity in Leukemic HL60 Cells. Toxicol In Vitro. 2015;29(7):1711-9. PubMed PMID: 26028147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Copper-based nanoparticles induce high toxicity in leukemic HL60 cells. AU - Rodhe,Ylva, AU - Skoglund,Sara, AU - Odnevall Wallinder,Inger, AU - Potácová,Zuzana, AU - Möller,Lennart, Y1 - 2015/05/28/ PY - 2014/06/11/received PY - 2015/05/25/revised PY - 2015/05/27/accepted PY - 2015/6/2/entrez PY - 2015/6/2/pubmed PY - 2016/6/23/medline KW - Cu KW - CuO KW - Cytotoxicity KW - Mitochondrial damage KW - Nanoparticles KW - Oxidative stress SP - 1711 EP - 9 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 29 IS - 7 N2 - From the increasing societal use of nanoparticles (NPs) follows the necessity to understand their potential toxic effects. This requires an in-depth understanding of the relationship between their physicochemical properties and their toxicological behavior. The aim of the present work was to study the toxicity of Cu and CuO NPs toward the leukemic cell line HL60. The toxicity was explored in terms of mitochondrial damage, DNA damage, oxidative DNA damage, cell death and reactive oxygen species (ROS) formation. Particle characteristics and copper release were specifically investigated in order to gain an improved understanding of prevailing toxic mechanisms. The Cu NPs revealed higher toxicity compared with both CuO NPs and dissolved copper (CuCl2), as well as a more rapid copper release compared with CuO NPs. Mitochondrial damage was induced by Cu NPs already after 2 h exposure. Cu NPs induced oxidation at high levels in an acellular ROS assay, and a small increase of intracellular ROS was observed. The increase of DNA damage was limited. CuO NPs did not induce any mitochondrial damage up to 6 h of exposure. No acellular ROS was induced by the CuO NPs, and the levels of intracellular ROS and DNA damage were limited after 2 h exposure. Necrosis was the main type of cell death observed after 18 h exposure to CuO NP and dissolved copper. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/26028147/Copper_based_nanoparticles_induce_high_toxicity_in_leukemic_HL60_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(15)00124-1 DB - PRIME DP - Unbound Medicine ER -