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Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection.
J Korean Med Sci. 2015 Jun; 30(6):816-22.JK

Abstract

Colloidal particle size is an important characteristic that allows mapping sentinel nodes in lymphoscintigraphy. This investigation aimed to introduce different ways of making a (99m)Tc-tin colloid with a size of tens of nanometers. All agents, tin fluoride, sodium fluoride, poloxamer-188, and polyvinylpyrrolidone (PVP), were mixed and labeled with (99m)Tc. Either phosphate or sodium bicarbonate buffers were used to adjust the pH levels. When the buffers were added, the size of the colloids increased. However, as the PVP continued to increase, the size of the colloids was controlled to within tens of nanometers. In all samples, phosphate buffer added PVP (30 mg) stabilized tin colloid ((99m)Tc-PPTC-30) and sodium bicarbonate solution added PVP (50 mg) stabilized tin colloid ((99m)Tc-BPTC-50) were chosen for in vitro and in vivo studies. (99m)Tc-BPTC-50 (<20 nm) was primarily located in bone marrow and was then secreted through the kidneys, and (99m)Tc-PPTC-30 (>100 nm) mainly accumulated in the liver. When a rabbit was given a toe injection, the node uptake of (99m)Tc-PPTC-30 decreased over time, while (99m)Tc-BPTC-50 increased. Therefore, (99m)Tc-BPTC-50 could be a good candidate radiopharmaceutical for sentinel node detection. The significance of this study is that nano-sized tin colloid can be made very easily and quickly by PVP.

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Authors+Show Affiliations

Kim EM
Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
Lim ST
Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
Sohn MH
Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.
Jeong HJ
Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Cyclotron Research Center, Institute for Medical Science, Biomedical Research Institute, Chonbuk National University Medical School and Hospital, Jeonju, Korea.

MeSH

AnimalsBuffersCell Line, TumorHumansLymph NodesLymphatic MetastasisMetal NanoparticlesMiceNeoplasms, ExperimentalParticle SizePovidoneRabbitsRadionuclide ImagingRadiopharmaceuticalsReproducibility of ResultsSensitivity and SpecificityTechnetium CompoundsTinTin Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26028937

Citation

Kim, Eun-Mi, et al. "Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection." Journal of Korean Medical Science, vol. 30, no. 6, 2015, pp. 816-22.
Kim EM, Lim ST, Sohn MH, et al. Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection. J Korean Med Sci. 2015;30(6):816-22.
Kim, E. M., Lim, S. T., Sohn, M. H., & Jeong, H. J. (2015). Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection. Journal of Korean Medical Science, 30(6), 816-22. https://doi.org/10.3346/jkms.2015.30.6.816
Kim EM, et al. Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection. J Korean Med Sci. 2015;30(6):816-22. PubMed PMID: 26028937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Size Control of (99m)Tc-tin Colloid Using PVP and Buffer Solution for Sentinel Lymph Node Detection. AU - Kim,Eun-Mi, AU - Lim,Seok Tae, AU - Sohn,Myung-Hee, AU - Jeong,Hwan-Jeong, Y1 - 2015/05/13/ PY - 2014/08/30/received PY - 2015/01/05/accepted PY - 2015/6/2/entrez PY - 2015/6/2/pubmed PY - 2016/3/15/medline KW - 99mTc-tin Colloid KW - Lymphoscintigraphy KW - Polyvinylpyrrolidone (PVP) KW - Sentinel Lymph Node SP - 816 EP - 22 JF - Journal of Korean medical science JO - J Korean Med Sci VL - 30 IS - 6 N2 - Colloidal particle size is an important characteristic that allows mapping sentinel nodes in lymphoscintigraphy. This investigation aimed to introduce different ways of making a (99m)Tc-tin colloid with a size of tens of nanometers. All agents, tin fluoride, sodium fluoride, poloxamer-188, and polyvinylpyrrolidone (PVP), were mixed and labeled with (99m)Tc. Either phosphate or sodium bicarbonate buffers were used to adjust the pH levels. When the buffers were added, the size of the colloids increased. However, as the PVP continued to increase, the size of the colloids was controlled to within tens of nanometers. In all samples, phosphate buffer added PVP (30 mg) stabilized tin colloid ((99m)Tc-PPTC-30) and sodium bicarbonate solution added PVP (50 mg) stabilized tin colloid ((99m)Tc-BPTC-50) were chosen for in vitro and in vivo studies. (99m)Tc-BPTC-50 (<20 nm) was primarily located in bone marrow and was then secreted through the kidneys, and (99m)Tc-PPTC-30 (>100 nm) mainly accumulated in the liver. When a rabbit was given a toe injection, the node uptake of (99m)Tc-PPTC-30 decreased over time, while (99m)Tc-BPTC-50 increased. Therefore, (99m)Tc-BPTC-50 could be a good candidate radiopharmaceutical for sentinel node detection. The significance of this study is that nano-sized tin colloid can be made very easily and quickly by PVP. SN - 1598-6357 UR - https://www.unboundmedicine.com/medline/citation/26028937/Size_Control_of__99m_Tc_tin_Colloid_Using_PVP_and_Buffer_Solution_for_Sentinel_Lymph_Node_Detection_ DB - PRIME DP - Unbound Medicine ER -