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Combination Patterns of Major R Genes Determine the Level of Resistance to the M. oryzae in Rice (Oryza sativa L.).
PLoS One 2015; 10(6):e0126130Plos

Abstract

Rice blast caused by Magnaporthe oryzae is the most devastating disease of rice and poses a serious threat to world food security. In this study, the distribution and effectiveness of 18 R genes in 277 accessions were investigated based on pathogenicity assays and molecular markers. The results showed that most of the accessions exhibited some degree of resistance (resistance frequency, RF >50%). Accordingly, most of the accessions were observed to harbor two or more R genes, and the number of R genes harbored in accessions was significantly positively correlated with RF. Some R genes were demonstrated to be specifically distributed in the genomes of rice sub-species, such as Pigm, Pi9, Pi5 and Pi1, which were only detected in indica-type accessions, and Pik and Piz, which were just harbored in japonica-type accessions. By analyzing the relationship between R genes and RF using a multiple stepwise regression model, the R genes Pid3, Pi5, Pi9, Pi54, Pigm and Pit were found to show the main effects against M. oryzae in indica-type accessions, while Pita, Pb1, Pik, Pizt and Pia were indicated to exhibit the main effects against M. oryzae in japonica-type accessions. Principal component analysis (PCA) and cluster analysis revealed that combination patterns of major R genes were the main factors determining the resistance of rice varieties to M. oryzae, such as 'Pi9+Pi54', 'Pid3+Pigm', 'Pi5+Pid3+Pigm', 'Pi5+Pi54+Pid3+Pigm', 'Pi5+Pid3' and 'Pi5+Pit+Pid3' in indica-type accessions and 'Pik+Pib', 'Pik+Pita', 'Pik+Pb1', 'Pizt+Pia' and 'Pizt+Pita' in japonica-type accessions, which were able to confer effective resistance against M. oryzae. The above results provide good theoretical support for the rational utilization of combinations of major R genes in developing rice cultivars with broad-spectrum resistance.

Authors+Show Affiliations

Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China; Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, 225009, P.R. China.Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou, 225007, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26030358

Citation

Wu, Yunyu, et al. "Combination Patterns of Major R Genes Determine the Level of Resistance to the M. Oryzae in Rice (Oryza Sativa L.)." PloS One, vol. 10, no. 6, 2015, pp. e0126130.
Wu Y, Xiao N, Yu L, et al. Combination Patterns of Major R Genes Determine the Level of Resistance to the M. oryzae in Rice (Oryza sativa L.). PLoS ONE. 2015;10(6):e0126130.
Wu, Y., Xiao, N., Yu, L., Pan, C., Li, Y., Zhang, X., ... Li, A. (2015). Combination Patterns of Major R Genes Determine the Level of Resistance to the M. oryzae in Rice (Oryza sativa L.). PloS One, 10(6), pp. e0126130. doi:10.1371/journal.pone.0126130.
Wu Y, et al. Combination Patterns of Major R Genes Determine the Level of Resistance to the M. Oryzae in Rice (Oryza Sativa L.). PLoS ONE. 2015;10(6):e0126130. PubMed PMID: 26030358.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combination Patterns of Major R Genes Determine the Level of Resistance to the M. oryzae in Rice (Oryza sativa L.). AU - Wu,Yunyu, AU - Xiao,Ning, AU - Yu,Ling, AU - Pan,Cunhong, AU - Li,Yuhong, AU - Zhang,Xiaoxiang, AU - Liu,Guangqing, AU - Dai,Zhengyuan, AU - Pan,Xuebiao, AU - Li,Aihong, Y1 - 2015/06/01/ PY - 2015/01/21/received PY - 2015/03/29/accepted PY - 2015/6/2/entrez PY - 2015/6/2/pubmed PY - 2016/3/5/medline SP - e0126130 EP - e0126130 JF - PloS one JO - PLoS ONE VL - 10 IS - 6 N2 - Rice blast caused by Magnaporthe oryzae is the most devastating disease of rice and poses a serious threat to world food security. In this study, the distribution and effectiveness of 18 R genes in 277 accessions were investigated based on pathogenicity assays and molecular markers. The results showed that most of the accessions exhibited some degree of resistance (resistance frequency, RF >50%). Accordingly, most of the accessions were observed to harbor two or more R genes, and the number of R genes harbored in accessions was significantly positively correlated with RF. Some R genes were demonstrated to be specifically distributed in the genomes of rice sub-species, such as Pigm, Pi9, Pi5 and Pi1, which were only detected in indica-type accessions, and Pik and Piz, which were just harbored in japonica-type accessions. By analyzing the relationship between R genes and RF using a multiple stepwise regression model, the R genes Pid3, Pi5, Pi9, Pi54, Pigm and Pit were found to show the main effects against M. oryzae in indica-type accessions, while Pita, Pb1, Pik, Pizt and Pia were indicated to exhibit the main effects against M. oryzae in japonica-type accessions. Principal component analysis (PCA) and cluster analysis revealed that combination patterns of major R genes were the main factors determining the resistance of rice varieties to M. oryzae, such as 'Pi9+Pi54', 'Pid3+Pigm', 'Pi5+Pid3+Pigm', 'Pi5+Pi54+Pid3+Pigm', 'Pi5+Pid3' and 'Pi5+Pit+Pid3' in indica-type accessions and 'Pik+Pib', 'Pik+Pita', 'Pik+Pb1', 'Pizt+Pia' and 'Pizt+Pita' in japonica-type accessions, which were able to confer effective resistance against M. oryzae. The above results provide good theoretical support for the rational utilization of combinations of major R genes in developing rice cultivars with broad-spectrum resistance. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26030358/Combination_Patterns_of_Major_R_Genes_Determine_the_Level_of_Resistance_to_the_M__oryzae_in_Rice__Oryza_sativa_L___ L2 - http://dx.plos.org/10.1371/journal.pone.0126130 DB - PRIME DP - Unbound Medicine ER -