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Determination and validation of hupehenine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study.
Biomed Chromatogr. 2015 Dec; 29(12):1805-10.BC

Abstract

In this work, a sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determination of hupehenine in rat plasma was developed and validated. After addition of imperialine as an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 × 100 mm, 1.7 µm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 416.3 → 98.0 for hupehenine, and m/z 430.3 → 138.2 for IS. Calibration plots were linear throughout the range 2-2000 ng/mL for hupehenine in rat plasma. Mean recoveries of hupehenine in rat plasma ranged from 92.5 to 97.3%. Relative standard deviations of intra-day and inter-day precision were both <6%. The accuracy of the method was between 92.7 and 107.4%. The method was successfully applied to a pharmacokinetic study of hupehenine after either oral or intravenous administration. For the first time, the bioavailability of hupehenine was reported as 13.4%.

Authors+Show Affiliations

Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui 323000, Wenzhou Medical University, Lishui, China.Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, 325035, China.Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.The Laboratory of Clinical Pharmacy, The People's Hospital of Lishui 323000, Wenzhou Medical University, Lishui, China.Analytical and Testing Center, Wenzhou Medical University, Wenzhou, 325035, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26033449

Citation

Wen, Congcong, et al. "Determination and Validation of Hupehenine in Rat Plasma By UPLC-MS/MS and Its Application to Pharmacokinetic Study." Biomedical Chromatography : BMC, vol. 29, no. 12, 2015, pp. 1805-10.
Wen C, Wang S, Huang X, et al. Determination and validation of hupehenine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study. Biomed Chromatogr. 2015;29(12):1805-10.
Wen, C., Wang, S., Huang, X., Liu, Z., Lin, Y., Yang, S., Ma, J., Zhou, Y., & Wang, X. (2015). Determination and validation of hupehenine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study. Biomedical Chromatography : BMC, 29(12), 1805-10. https://doi.org/10.1002/bmc.3499
Wen C, et al. Determination and Validation of Hupehenine in Rat Plasma By UPLC-MS/MS and Its Application to Pharmacokinetic Study. Biomed Chromatogr. 2015;29(12):1805-10. PubMed PMID: 26033449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination and validation of hupehenine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study. AU - Wen,Congcong, AU - Wang,Shuanghu, AU - Huang,Xueli, AU - Liu,Zezheng, AU - Lin,Yingying, AU - Yang,Suping, AU - Ma,Jianshe, AU - Zhou,Yunfang, AU - Wang,Xianqin, Y1 - 2015/06/01/ PY - 2015/01/08/received PY - 2015/03/22/revised PY - 2015/04/23/accepted PY - 2015/6/3/entrez PY - 2015/6/3/pubmed PY - 2016/5/19/medline KW - UPLC-MS/MS KW - hupehenine KW - pharmacokinetics KW - rat SP - 1805 EP - 10 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 29 IS - 12 N2 - In this work, a sensitive and selective ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determination of hupehenine in rat plasma was developed and validated. After addition of imperialine as an internal standard (IS), protein precipitation by acetonitrile-methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 column (2.1 × 100 mm, 1.7 µm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m/z 416.3 → 98.0 for hupehenine, and m/z 430.3 → 138.2 for IS. Calibration plots were linear throughout the range 2-2000 ng/mL for hupehenine in rat plasma. Mean recoveries of hupehenine in rat plasma ranged from 92.5 to 97.3%. Relative standard deviations of intra-day and inter-day precision were both <6%. The accuracy of the method was between 92.7 and 107.4%. The method was successfully applied to a pharmacokinetic study of hupehenine after either oral or intravenous administration. For the first time, the bioavailability of hupehenine was reported as 13.4%. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/26033449/Determination_and_validation_of_hupehenine_in_rat_plasma_by_UPLC_MS/MS_and_its_application_to_pharmacokinetic_study_ L2 - https://doi.org/10.1002/bmc.3499 DB - PRIME DP - Unbound Medicine ER -