Tags

Type your tag names separated by a space and hit enter

An H5N1-based matrix protein 2 ectodomain tetrameric peptide vaccine provides cross-protection against lethal infection with H7N9 influenza virus.
Emerg Microbes Infect. 2015 Apr; 4(4):e22.EM

Abstract

In March 2013, a patient infected with a novel avian influenza A H7N9 virus was reported in China. Since then, there have been 458 confirmed infection cases and 177 deaths. The virus contains several human-adapted markers, indicating that H7N9 has pandemic potential. The outbreak of this new influenza virus highlighted the need for the development of universal influenza vaccines. Previously, we demonstrated that a tetrameric peptide vaccine based on the matrix protein 2 ectodomain (M2e) of the H5N1 virus (H5N1-M2e) could protect mice from lethal infection with different clades of H5N1 and 2009 pandemic H1N1 influenza viruses. In this study, we investigated the cross-protection of H5N1-M2e against lethal infection with the new H7N9 virus. Although five amino acid differences existed at positions 13, 14, 18, 20, and 21 between M2e of H5N1 and H7N9, H5N1-M2e vaccination with either Freund's adjuvant or the Sigma adjuvant system (SAS) induced a high level of anti-M2e antibody, which cross-reacted with H7N9-M2e peptide. A mouse-adapted H7N9 strain, A/Anhui/01/2013m, was used for lethal challenge in animal experiments. H5N1-M2e vaccination provided potent cross-protection against lethal challenge of the H7N9 virus. Reduced viral replication and histopathological damage of mouse lungs were also observed in the vaccinated mice. Our results suggest that the tetrameric H5N1-M2e peptide vaccine could protect against different subtypes of influenza virus infections. Therefore, this vaccine may be an ideal candidate for developing a universal vaccine to prevent the reemergence of avian influenza A H7N9 virus and the emergence of potential novel reassortants of influenza virus.

Authors+Show Affiliations

Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.Department of Microbiology, University of Hong Kong , Hong Kong, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26038770

Citation

Leung, Ho-Chuen, et al. "An H5N1-based Matrix Protein 2 Ectodomain Tetrameric Peptide Vaccine Provides Cross-protection Against Lethal Infection With H7N9 Influenza Virus." Emerging Microbes & Infections, vol. 4, no. 4, 2015, pp. e22.
Leung HC, Chan CC, Poon VK, et al. An H5N1-based matrix protein 2 ectodomain tetrameric peptide vaccine provides cross-protection against lethal infection with H7N9 influenza virus. Emerg Microbes Infect. 2015;4(4):e22.
Leung, H. C., Chan, C. C., Poon, V. K., Zhao, H. J., Cheung, C. Y., Ng, F., Huang, J. D., & Zheng, B. J. (2015). An H5N1-based matrix protein 2 ectodomain tetrameric peptide vaccine provides cross-protection against lethal infection with H7N9 influenza virus. Emerging Microbes & Infections, 4(4), e22. https://doi.org/10.1038/emi.2015.22
Leung HC, et al. An H5N1-based Matrix Protein 2 Ectodomain Tetrameric Peptide Vaccine Provides Cross-protection Against Lethal Infection With H7N9 Influenza Virus. Emerg Microbes Infect. 2015;4(4):e22. PubMed PMID: 26038770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An H5N1-based matrix protein 2 ectodomain tetrameric peptide vaccine provides cross-protection against lethal infection with H7N9 influenza virus. AU - Leung,Ho-Chuen, AU - Chan,Chris Chung-Sing, AU - Poon,Vincent Kwok-Man, AU - Zhao,Han-Jun, AU - Cheung,Chung-Yan, AU - Ng,Fai, AU - Huang,Jian-Dong, AU - Zheng,Bo-Jian, Y1 - 2015/04/08/ PY - 2015/02/01/received PY - 2015/02/27/revised PY - 2015/03/01/accepted PY - 2015/6/4/entrez PY - 2015/6/4/pubmed PY - 2015/6/4/medline KW - H7N9 KW - M2e KW - cross protection KW - influenza A virus KW - peptide vaccine SP - e22 EP - e22 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 4 IS - 4 N2 - In March 2013, a patient infected with a novel avian influenza A H7N9 virus was reported in China. Since then, there have been 458 confirmed infection cases and 177 deaths. The virus contains several human-adapted markers, indicating that H7N9 has pandemic potential. The outbreak of this new influenza virus highlighted the need for the development of universal influenza vaccines. Previously, we demonstrated that a tetrameric peptide vaccine based on the matrix protein 2 ectodomain (M2e) of the H5N1 virus (H5N1-M2e) could protect mice from lethal infection with different clades of H5N1 and 2009 pandemic H1N1 influenza viruses. In this study, we investigated the cross-protection of H5N1-M2e against lethal infection with the new H7N9 virus. Although five amino acid differences existed at positions 13, 14, 18, 20, and 21 between M2e of H5N1 and H7N9, H5N1-M2e vaccination with either Freund's adjuvant or the Sigma adjuvant system (SAS) induced a high level of anti-M2e antibody, which cross-reacted with H7N9-M2e peptide. A mouse-adapted H7N9 strain, A/Anhui/01/2013m, was used for lethal challenge in animal experiments. H5N1-M2e vaccination provided potent cross-protection against lethal challenge of the H7N9 virus. Reduced viral replication and histopathological damage of mouse lungs were also observed in the vaccinated mice. Our results suggest that the tetrameric H5N1-M2e peptide vaccine could protect against different subtypes of influenza virus infections. Therefore, this vaccine may be an ideal candidate for developing a universal vaccine to prevent the reemergence of avian influenza A H7N9 virus and the emergence of potential novel reassortants of influenza virus. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/26038770/An_H5N1_based_matrix_protein_2_ectodomain_tetrameric_peptide_vaccine_provides_cross_protection_against_lethal_infection_with_H7N9_influenza_virus_ L2 - https://www.tandfonline.com/doi/full/10.1038/emi.2015.22 DB - PRIME DP - Unbound Medicine ER -