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Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization.
Ann Bot. 2015 Aug; 116(2):189-200.AB

Abstract

BACKGROUND AND AIMS

Aegilops markgrafii (CC) and its natural hybrids Ae. triuncialis (U(t)U(t)C(t)C(t)) and Ae. cylindrica (D(c)D(c)C(c)C(c)) represent a rich reservoir of useful genes for improvement of bread wheat (Triticum aestivum), but the limited information available on their genome structure and the shortage of molecular (cyto-) genetic tools hamper the utilization of the extant genetic diversity. This study provides the complete karyotypes in the three species obtained after fluorescent in situ hybridization (FISH) with repetitive DNA probes, and evaluates the potential of flow cytometric chromosome sorting.

METHODS

The flow karyotypes obtained after the analysis of 4',6-diamidino-2-phenylindole (DAPI)-stained chromosomes were characterized and the chromosome content of the peaks on the flow karyotypes was determined by FISH. Twenty-nine conserved orthologous set (COS) markers covering all seven wheat homoeologous chromosome groups were used for PCR with DNA amplified from flow-sorted chromosomes and genomic DNA.

KEY RESULTS

FISH with repetitive DNA probes revealed that chromosomes 4C, 5C, 7C(t), T6U(t)S.6U(t)L-5C(t)L, 1C(c) and 5D(c) could be sorted with purities ranging from 66 to 91 %, while the remaining chromosomes could be sorted in groups of 2-5. This identified a partial wheat-C-genome homology for group 4 and 5 chromosomes. In addition, 1C chromosomes were homologous with group 1 of wheat; a small segment from group 2 indicated 1C-2C rearrangement. An extensively rearranged structure of chromosome 7C relative to wheat was also detected.

CONCLUSIONS

The possibility of purifying Aegilops chromosomes provides an attractive opportunity to investigate the structure and evolution of the Aegilops C genome and to develop molecular tools to facilitate the identification of alien chromatin and support alien introgression breeding in bread wheat.

Authors+Show Affiliations

Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, H-2462 Martonvásár, Hungary and molnar.istvan@agrar.mta.hu.Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, CZ-78371 Olomouc, Czech Republic.Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, H-2462 Martonvásár, Hungary and.Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, CZ-78371 Olomouc, Czech Republic.Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, H-2462 Martonvásár, Hungary and.Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, H-2462 Martonvásár, Hungary and.Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, CZ-78371 Olomouc, Czech Republic.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26043745

Citation

Molnár, István, et al. "Flow Sorting of C-genome Chromosomes From Wild Relatives of Wheat Aegilops Markgrafii, Ae. Triuncialis and Ae. Cylindrica, and Their Molecular Organization." Annals of Botany, vol. 116, no. 2, 2015, pp. 189-200.
Molnár I, Vrána J, Farkas A, et al. Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization. Ann Bot. 2015;116(2):189-200.
Molnár, I., Vrána, J., Farkas, A., Kubaláková, M., Cseh, A., Molnár-Láng, M., & Doležel, J. (2015). Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization. Annals of Botany, 116(2), 189-200. https://doi.org/10.1093/aob/mcv073
Molnár I, et al. Flow Sorting of C-genome Chromosomes From Wild Relatives of Wheat Aegilops Markgrafii, Ae. Triuncialis and Ae. Cylindrica, and Their Molecular Organization. Ann Bot. 2015;116(2):189-200. PubMed PMID: 26043745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization. AU - Molnár,István, AU - Vrána,Jan, AU - Farkas,András, AU - Kubaláková,Marie, AU - Cseh,András, AU - Molnár-Láng,Márta, AU - Doležel,Jaroslav, Y1 - 2015/06/04/ PY - 2015/02/02/received PY - 2015/04/15/accepted PY - 2015/6/6/entrez PY - 2015/6/6/pubmed PY - 2016/4/19/medline KW - Ae. cylindrica KW - Ae. triuncialis KW - Aegilops markgrafii KW - COS KW - FISH KW - GISH KW - Poaceae KW - Triticum aestivum KW - conserved orthologous set markers KW - flow cytometric chromosome sorting KW - fluorescence in situ hybridization KW - genomic in situ hybridization KW - goatgrass KW - physical mapping KW - wheat SP - 189 EP - 200 JF - Annals of botany JO - Ann Bot VL - 116 IS - 2 N2 - BACKGROUND AND AIMS: Aegilops markgrafii (CC) and its natural hybrids Ae. triuncialis (U(t)U(t)C(t)C(t)) and Ae. cylindrica (D(c)D(c)C(c)C(c)) represent a rich reservoir of useful genes for improvement of bread wheat (Triticum aestivum), but the limited information available on their genome structure and the shortage of molecular (cyto-) genetic tools hamper the utilization of the extant genetic diversity. This study provides the complete karyotypes in the three species obtained after fluorescent in situ hybridization (FISH) with repetitive DNA probes, and evaluates the potential of flow cytometric chromosome sorting. METHODS: The flow karyotypes obtained after the analysis of 4',6-diamidino-2-phenylindole (DAPI)-stained chromosomes were characterized and the chromosome content of the peaks on the flow karyotypes was determined by FISH. Twenty-nine conserved orthologous set (COS) markers covering all seven wheat homoeologous chromosome groups were used for PCR with DNA amplified from flow-sorted chromosomes and genomic DNA. KEY RESULTS: FISH with repetitive DNA probes revealed that chromosomes 4C, 5C, 7C(t), T6U(t)S.6U(t)L-5C(t)L, 1C(c) and 5D(c) could be sorted with purities ranging from 66 to 91 %, while the remaining chromosomes could be sorted in groups of 2-5. This identified a partial wheat-C-genome homology for group 4 and 5 chromosomes. In addition, 1C chromosomes were homologous with group 1 of wheat; a small segment from group 2 indicated 1C-2C rearrangement. An extensively rearranged structure of chromosome 7C relative to wheat was also detected. CONCLUSIONS: The possibility of purifying Aegilops chromosomes provides an attractive opportunity to investigate the structure and evolution of the Aegilops C genome and to develop molecular tools to facilitate the identification of alien chromatin and support alien introgression breeding in bread wheat. SN - 1095-8290 UR - https://www.unboundmedicine.com/medline/citation/26043745/Flow_sorting_of_C_genome_chromosomes_from_wild_relatives_of_wheat_Aegilops_markgrafii_Ae__triuncialis_and_Ae__cylindrica_and_their_molecular_organization_ L2 - https://academic.oup.com/aob/article-lookup/doi/10.1093/aob/mcv073 DB - PRIME DP - Unbound Medicine ER -