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Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry.
Rapid Commun Mass Spectrom. 2015 Jun 15; 29(11):1069-79.RC

Abstract

RATIONALE

It has been proposed that malondialdehyde (MDA) reflects free oxygen-radical lipid peroxidation and can be useful as a biomarker to track this process. For the analysis of MDA molecules in humid air by selected ion flow tube mass spectrometry (SIFT-MS), the rate coefficients and the ion product distributions for the reactions of the SIFT-MS reagent ions with volatile MDA in the presence of water vapour are required.

METHODS

The SIFT technique has been used to determine the rate coefficients and ion product distributions for the reactions of H3O(+), NO(+) and O2 (+•) with gas-phase MDA. In support of the SIFT-MS analysis of MDA, solid-phase microextraction, SPME, coupled with gas chromatography/mass spectrometry, GC/MS, has been used to confirm the identification of MDA.

RESULTS

The primary product ions have been identified for the reactions of H3O(+), NO(+) and O2 (+•) with MDA and the formation of their hydrates formed in humid samples is described. The following combinations of reagent and the analyte ions (given as m/z values) have been adopted for SIFT-MS analyses of MDA in the gas phase: H3O(+): 109; NO(+): 89, 102; O2 (+•): 72, 90, 108, 126. The detection and quantification of MDA released by a cell culture by SIFT-MS are demonstrated.

CONCLUSIONS

This detailed study has provided the kinetics data required for the SIFT-MS analysis of MDA in humid air, including exhaled breath and the headspace of liquid-phase biogenic media. The detection and quantification by SIFT-MS of MDA released by a cell culture are demonstrated.

Authors+Show Affiliations

J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic.Department of Surgery and Cancer, Imperial College London, St. Mary's Hospital, Praed Street, London, W2 1NY, UK.J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic.J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic.Institute for Science and Technology in Medicine, School of Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK.J. Heyrovsky Institute of Physical Chemistry of Science, Academy of Science of the Czech Republic, Dolejškova 3, 18223, Prague 8, Czech Republic. Institute for Science and Technology in Medicine, School of Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent, ST4 7QB, UK.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26044275

Citation

Shestivska, Violetta, et al. "Direct Detection and Quantification of Malondialdehyde Vapour in Humid Air Using Selected Ion Flow Tube Mass Spectrometry Supported By Gas Chromatography/mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 29, no. 11, 2015, pp. 1069-79.
Shestivska V, Antonowicz SS, Dryahina K, et al. Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom. 2015;29(11):1069-79.
Shestivska, V., Antonowicz, S. S., Dryahina, K., Kubišta, J., Smith, D., & Španěl, P. (2015). Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 29(11), 1069-79. https://doi.org/10.1002/rcm.7198
Shestivska V, et al. Direct Detection and Quantification of Malondialdehyde Vapour in Humid Air Using Selected Ion Flow Tube Mass Spectrometry Supported By Gas Chromatography/mass Spectrometry. Rapid Commun Mass Spectrom. 2015 Jun 15;29(11):1069-79. PubMed PMID: 26044275.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct detection and quantification of malondialdehyde vapour in humid air using selected ion flow tube mass spectrometry supported by gas chromatography/mass spectrometry. AU - Shestivska,Violetta, AU - Antonowicz,Stefan S, AU - Dryahina,Kseniya, AU - Kubišta,Jiří, AU - Smith,David, AU - Španěl,Patrik, PY - 2015/01/26/received PY - 2015/03/12/revised PY - 2015/03/12/accepted PY - 2015/6/6/entrez PY - 2015/6/6/pubmed PY - 2016/2/11/medline SP - 1069 EP - 79 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 29 IS - 11 N2 - RATIONALE: It has been proposed that malondialdehyde (MDA) reflects free oxygen-radical lipid peroxidation and can be useful as a biomarker to track this process. For the analysis of MDA molecules in humid air by selected ion flow tube mass spectrometry (SIFT-MS), the rate coefficients and the ion product distributions for the reactions of the SIFT-MS reagent ions with volatile MDA in the presence of water vapour are required. METHODS: The SIFT technique has been used to determine the rate coefficients and ion product distributions for the reactions of H3O(+), NO(+) and O2 (+•) with gas-phase MDA. In support of the SIFT-MS analysis of MDA, solid-phase microextraction, SPME, coupled with gas chromatography/mass spectrometry, GC/MS, has been used to confirm the identification of MDA. RESULTS: The primary product ions have been identified for the reactions of H3O(+), NO(+) and O2 (+•) with MDA and the formation of their hydrates formed in humid samples is described. The following combinations of reagent and the analyte ions (given as m/z values) have been adopted for SIFT-MS analyses of MDA in the gas phase: H3O(+): 109; NO(+): 89, 102; O2 (+•): 72, 90, 108, 126. The detection and quantification of MDA released by a cell culture by SIFT-MS are demonstrated. CONCLUSIONS: This detailed study has provided the kinetics data required for the SIFT-MS analysis of MDA in humid air, including exhaled breath and the headspace of liquid-phase biogenic media. The detection and quantification by SIFT-MS of MDA released by a cell culture are demonstrated. SN - 1097-0231 UR - https://www.unboundmedicine.com/medline/citation/26044275/Direct_detection_and_quantification_of_malondialdehyde_vapour_in_humid_air_using_selected_ion_flow_tube_mass_spectrometry_supported_by_gas_chromatography/mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.7198 DB - PRIME DP - Unbound Medicine ER -