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Corticotropin-releasing factor enhances inhibitory synaptic transmission to type III neurons in the bed nucleus of the stria terminalis.
Neurosci Lett 2015; 600:56-61NL

Abstract

We previously reported that corticotropin-releasing factor (CRF) increased neuronal excitability specifically in type II neurons of the dorsolateral part of the bed nucleus of the stria terminalis (dlBNST). Because the majority of type II dlBNST neurons are thought to be GABAergic interneurons, at least a portion of which are considered to regulate type III dlBNST neurons, it is possible that CRF increases inhibitory input to type III neurons through the activation of type II neurons in the dlBNST. To test this possibility, we examined the effect of CRF on type III dlBNST neurons using whole-cell voltage-clamp recordings of inhibitory postsynaptic currents (IPSCs) from rat BNST slices in the presence of kynurenic acid. Spontaneous IPSCs (sIPSCs) and miniature IPSCs (mIPSCs) were recorded in the absence and presence of tetrodotoxin, respectively. Bath application of CRF significantly increased the frequency of sIPSCs, indicating that CRF enhances the inhibitory input to type III neurons. CRF application failed to increase the frequency of mIPSCs, suggesting that CRF-induced increases in sIPSCs are dependent on action potentials. Combined with our previous finding that CRF specifically depolarizes type II dlBNST neurons, these results suggest that CRF may attenuate type III neuron excitation by augmenting the inhibitory influence of type II neurons in the dlBNST.

Authors+Show Affiliations

Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.Center for the Neuroscience of Pain and Department of Neuroscience, Jikei University School of Medicine, Tokyo 105-8461, Japan.Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan. Electronic address: mminami@pharm.hokudai.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26044990

Citation

Nagano, Yusuke, et al. "Corticotropin-releasing Factor Enhances Inhibitory Synaptic Transmission to Type III Neurons in the Bed Nucleus of the Stria Terminalis." Neuroscience Letters, vol. 600, 2015, pp. 56-61.
Nagano Y, Kaneda K, Maruyama C, et al. Corticotropin-releasing factor enhances inhibitory synaptic transmission to type III neurons in the bed nucleus of the stria terminalis. Neurosci Lett. 2015;600:56-61.
Nagano, Y., Kaneda, K., Maruyama, C., Ide, S., Kato, F., & Minami, M. (2015). Corticotropin-releasing factor enhances inhibitory synaptic transmission to type III neurons in the bed nucleus of the stria terminalis. Neuroscience Letters, 600, pp. 56-61. doi:10.1016/j.neulet.2015.05.059.
Nagano Y, et al. Corticotropin-releasing Factor Enhances Inhibitory Synaptic Transmission to Type III Neurons in the Bed Nucleus of the Stria Terminalis. Neurosci Lett. 2015 Jul 23;600:56-61. PubMed PMID: 26044990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Corticotropin-releasing factor enhances inhibitory synaptic transmission to type III neurons in the bed nucleus of the stria terminalis. AU - Nagano,Yusuke, AU - Kaneda,Katsuyuki, AU - Maruyama,Chikashi, AU - Ide,Soichiro, AU - Kato,Fusao, AU - Minami,Masabumi, Y1 - 2015/06/01/ PY - 2015/03/16/received PY - 2015/05/18/revised PY - 2015/05/29/accepted PY - 2015/6/6/entrez PY - 2015/6/6/pubmed PY - 2015/10/20/medline KW - Bed nucleus of the stria terminalis KW - Corticotropin-releasing factor KW - Emotion KW - Extended amygdala KW - GABAergic KW - IPSC SP - 56 EP - 61 JF - Neuroscience letters JO - Neurosci. Lett. VL - 600 N2 - We previously reported that corticotropin-releasing factor (CRF) increased neuronal excitability specifically in type II neurons of the dorsolateral part of the bed nucleus of the stria terminalis (dlBNST). Because the majority of type II dlBNST neurons are thought to be GABAergic interneurons, at least a portion of which are considered to regulate type III dlBNST neurons, it is possible that CRF increases inhibitory input to type III neurons through the activation of type II neurons in the dlBNST. To test this possibility, we examined the effect of CRF on type III dlBNST neurons using whole-cell voltage-clamp recordings of inhibitory postsynaptic currents (IPSCs) from rat BNST slices in the presence of kynurenic acid. Spontaneous IPSCs (sIPSCs) and miniature IPSCs (mIPSCs) were recorded in the absence and presence of tetrodotoxin, respectively. Bath application of CRF significantly increased the frequency of sIPSCs, indicating that CRF enhances the inhibitory input to type III neurons. CRF application failed to increase the frequency of mIPSCs, suggesting that CRF-induced increases in sIPSCs are dependent on action potentials. Combined with our previous finding that CRF specifically depolarizes type II dlBNST neurons, these results suggest that CRF may attenuate type III neuron excitation by augmenting the inhibitory influence of type II neurons in the dlBNST. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/26044990/Corticotropin_releasing_factor_enhances_inhibitory_synaptic_transmission_to_type_III_neurons_in_the_bed_nucleus_of_the_stria_terminalis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(15)00426-7 DB - PRIME DP - Unbound Medicine ER -