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Supratidal Extremophiles--Cyanobacterial Diversity in the Rock Pools of the Croatian Adria.
Microb Ecol. 2015 Nov; 70(4):876-88.ME

Abstract

Hardly any molecular studies have been done on euendoliths of marine coastal environments, especially along the supratidal ranges of carbonate coasts. In our study, we provide a comparative sequence analysis using 454 pyrosequencing of the 16S ribosomal RNA (rRNA) gene combined with extensive microscopy of the endolithic community from rock pools of the Croatian Adria. Molecular diversity indices and richness estimates showed high level of diversity, particularly in high-salinity samples. The most common cyanobacteria belong to the order Pleurocapsales, similar to observations on limestone coasts in other parts of the world. Using single-cell amplification sequences of Hormathonema spp., Hyella caespitosa, and Kyrtuthrix dalmatica was for the first time introduced to the public GenBank.Microscopic investigations did not show qualitative variances in diversity among sites with different salinity but clear differences in prevalent organisms from similar environments suggesting that most of them are adapted to inhabit extreme, marine endolithic habitats and that similar species inhabit geographically separated but ecologically similar environments. This is a remarkable concordance rather seldom seen in molecular community studies in support of the hypothesis that endolithic ecosystems are seeded from a global meta-community.The relative diversity of the community is greater than those described from harsh endolithic habitats of cold and hot deserts. The maximum likelihood phylogenetic tree consisting of 166 operational taxonomic units (OTUs) at 96 % sequence similarity revealed 11 distinct clusters. Three clusters contained only epilithic or endolithic taxa, and five clusters contained mixed epilithic and endolithic taxa. Organisms clustered according to their taxonomic affiliations and not to their preferences to salt concentrations.

Authors+Show Affiliations

IBU, Plants Biodiversity and Evolution, CvO University of Oldenburg, D-26111, Oldenburg, Germany.IBU, Plants Biodiversity and Evolution, CvO University of Oldenburg, D-26111, Oldenburg, Germany.IBU, Plants Biodiversity and Evolution, CvO University of Oldenburg, D-26111, Oldenburg, Germany.IBU, Plants Biodiversity and Evolution, CvO University of Oldenburg, D-26111, Oldenburg, Germany.IBU, Functional Ecology of Plants, University of Oldenburg, D-26111, Oldenburg, Germany.Biological Science Center, Boston University, 5 Cummington Street, Boston, MA, 02215, USA.IBU, Plants Biodiversity and Evolution, CvO University of Oldenburg, D-26111, Oldenburg, Germany. katarzyna.palinska@uni-oldenburg.de.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26048370

Citation

Brandes, Maria, et al. "Supratidal Extremophiles--Cyanobacterial Diversity in the Rock Pools of the Croatian Adria." Microbial Ecology, vol. 70, no. 4, 2015, pp. 876-88.
Brandes M, Albach DC, Vogt JC, et al. Supratidal Extremophiles--Cyanobacterial Diversity in the Rock Pools of the Croatian Adria. Microb Ecol. 2015;70(4):876-88.
Brandes, M., Albach, D. C., Vogt, J. C., Mayland-Quellhorst, E., Mendieta-Leiva, G., Golubic, S., & Palinska, K. A. (2015). Supratidal Extremophiles--Cyanobacterial Diversity in the Rock Pools of the Croatian Adria. Microbial Ecology, 70(4), 876-88. https://doi.org/10.1007/s00248-015-0637-0
Brandes M, et al. Supratidal Extremophiles--Cyanobacterial Diversity in the Rock Pools of the Croatian Adria. Microb Ecol. 2015;70(4):876-88. PubMed PMID: 26048370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Supratidal Extremophiles--Cyanobacterial Diversity in the Rock Pools of the Croatian Adria. AU - Brandes,Maria, AU - Albach,Dirk C, AU - Vogt,Janina C, AU - Mayland-Quellhorst,Eike, AU - Mendieta-Leiva,Glenda, AU - Golubic,Stjepko, AU - Palinska,Katarzyna A, Y1 - 2015/06/07/ PY - 2015/02/21/received PY - 2015/05/29/accepted PY - 2015/6/7/entrez PY - 2015/6/7/pubmed PY - 2016/9/22/medline KW - Cyanobacteria KW - Endoliths KW - Pyrosequencing KW - Rare biosphere SP - 876 EP - 88 JF - Microbial ecology JO - Microb Ecol VL - 70 IS - 4 N2 - Hardly any molecular studies have been done on euendoliths of marine coastal environments, especially along the supratidal ranges of carbonate coasts. In our study, we provide a comparative sequence analysis using 454 pyrosequencing of the 16S ribosomal RNA (rRNA) gene combined with extensive microscopy of the endolithic community from rock pools of the Croatian Adria. Molecular diversity indices and richness estimates showed high level of diversity, particularly in high-salinity samples. The most common cyanobacteria belong to the order Pleurocapsales, similar to observations on limestone coasts in other parts of the world. Using single-cell amplification sequences of Hormathonema spp., Hyella caespitosa, and Kyrtuthrix dalmatica was for the first time introduced to the public GenBank.Microscopic investigations did not show qualitative variances in diversity among sites with different salinity but clear differences in prevalent organisms from similar environments suggesting that most of them are adapted to inhabit extreme, marine endolithic habitats and that similar species inhabit geographically separated but ecologically similar environments. This is a remarkable concordance rather seldom seen in molecular community studies in support of the hypothesis that endolithic ecosystems are seeded from a global meta-community.The relative diversity of the community is greater than those described from harsh endolithic habitats of cold and hot deserts. The maximum likelihood phylogenetic tree consisting of 166 operational taxonomic units (OTUs) at 96 % sequence similarity revealed 11 distinct clusters. Three clusters contained only epilithic or endolithic taxa, and five clusters contained mixed epilithic and endolithic taxa. Organisms clustered according to their taxonomic affiliations and not to their preferences to salt concentrations. SN - 1432-184X UR - https://www.unboundmedicine.com/medline/citation/26048370/Supratidal_Extremophiles__Cyanobacterial_Diversity_in_the_Rock_Pools_of_the_Croatian_Adria_ L2 - https://dx.doi.org/10.1007/s00248-015-0637-0 DB - PRIME DP - Unbound Medicine ER -