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Preparation and characterization of vanillin-crosslinked chitosan therapeutic bioactive microcarriers.
Int J Biol Macromol. 2015 Aug; 79:736-47.IJ

Abstract

Chitosan microspheres with diameter of 14.3-48.5 μm were prepared by emulsion method and using natural vanillin as cross-linking agent. The surface morphology and microstructure of the microspheres were characterized by scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy, etc. The hollow microspheres showed a well-defined spherical shape with median diameter of 30.3 μm and possessed a uniform surface with micro-wrinkles, which is in favor of the drug release. Interpenetrating network cross-linking mechanism might result from the Schiff base reaction and the acetalization of hydroxyl and carbonyl between chitosan and vanillin. Berberine, as a model drug, was loaded in the microspheres and released in a sustainable manner. The drug loading ratio could change from 9.16% to 29.70% corresponding to the entrapment efficiency of 91.61% to 74.25%. In vitro cell culture study using MG63 cells and in vivo implantation clearly showed that the microspheres could provide favorable cell attachment and biocompatibility. The results confirm that the drug-loaded vanillin-crosslinked chitosan microspheres could be a worthy candidate either as carriers of drugs and cells, or as therapeutic matrix for bone repair and regeneration.

Authors+Show Affiliations

Research Center for Nano-Biomaterials, Analytical & Testing Center, Sichuan University, Chengdu 610064, China.Department of Materials Science & Engineering, Chengdu University of Technology, Chengdu 610059, China.Research Center for Nano-Biomaterials, Analytical & Testing Center, Sichuan University, Chengdu 610064, China. Electronic address: nic7504@scu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26051343

Citation

Zou, Qin, et al. "Preparation and Characterization of Vanillin-crosslinked Chitosan Therapeutic Bioactive Microcarriers." International Journal of Biological Macromolecules, vol. 79, 2015, pp. 736-47.
Zou Q, Li J, Li Y. Preparation and characterization of vanillin-crosslinked chitosan therapeutic bioactive microcarriers. Int J Biol Macromol. 2015;79:736-47.
Zou, Q., Li, J., & Li, Y. (2015). Preparation and characterization of vanillin-crosslinked chitosan therapeutic bioactive microcarriers. International Journal of Biological Macromolecules, 79, 736-47. https://doi.org/10.1016/j.ijbiomac.2015.05.037
Zou Q, Li J, Li Y. Preparation and Characterization of Vanillin-crosslinked Chitosan Therapeutic Bioactive Microcarriers. Int J Biol Macromol. 2015;79:736-47. PubMed PMID: 26051343.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and characterization of vanillin-crosslinked chitosan therapeutic bioactive microcarriers. AU - Zou,Qin, AU - Li,Junfeng, AU - Li,Yubao, Y1 - 2015/06/04/ PY - 2014/12/27/received PY - 2015/05/14/revised PY - 2015/05/21/accepted PY - 2015/6/9/entrez PY - 2015/6/9/pubmed PY - 2016/4/26/medline KW - Biocompatibility KW - Drug delivery KW - Vanillin-crosslinked chitosan microspheres SP - 736 EP - 47 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 79 N2 - Chitosan microspheres with diameter of 14.3-48.5 μm were prepared by emulsion method and using natural vanillin as cross-linking agent. The surface morphology and microstructure of the microspheres were characterized by scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy, etc. The hollow microspheres showed a well-defined spherical shape with median diameter of 30.3 μm and possessed a uniform surface with micro-wrinkles, which is in favor of the drug release. Interpenetrating network cross-linking mechanism might result from the Schiff base reaction and the acetalization of hydroxyl and carbonyl between chitosan and vanillin. Berberine, as a model drug, was loaded in the microspheres and released in a sustainable manner. The drug loading ratio could change from 9.16% to 29.70% corresponding to the entrapment efficiency of 91.61% to 74.25%. In vitro cell culture study using MG63 cells and in vivo implantation clearly showed that the microspheres could provide favorable cell attachment and biocompatibility. The results confirm that the drug-loaded vanillin-crosslinked chitosan microspheres could be a worthy candidate either as carriers of drugs and cells, or as therapeutic matrix for bone repair and regeneration. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/26051343/Preparation_and_characterization_of_vanillin_crosslinked_chitosan_therapeutic_bioactive_microcarriers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(15)00378-5 DB - PRIME DP - Unbound Medicine ER -