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Energetic costs of protein synthesis do not differ between red- and white-blooded Antarctic notothenioid fishes.

Abstract

Antarctic icefishes (Family Channichthyidae) within the suborder Notothenioidei lack the oxygen-binding protein hemoglobin (Hb), and six of the 16 species of icefishes lack myoglobin (Mb) in heart ventricle. As iron-centered proteins, Hb and Mb can promote the formation of reactive oxygen species (ROS) that damage biological macromolecules. Consistent with this, our previous studies have shown that icefishes have lower levels of oxidized proteins and lipids in oxidative muscle compared to red-blooded notothenioids. Because oxidized proteins are usually degraded by the 20S proteasome and must be resynthesized, we hypothesized that rates of protein synthesis would be lower in icefishes compared to red-blooded notothenioids, thereby reducing the energetic costs of protein synthesis and conferring a benefit to the loss of Hb and Mb. Rates of protein synthesis were quantified in hearts, and the fraction of oxygen consumption devoted to protein synthesis was measured in isolated hepatocytes and cardiomyocytes of notothenioids differing in the expression of Hb and cardiac Mb. Neither rates of protein synthesis nor the energetic costs of protein synthesis differed among species, suggesting that red-blooded species do not degrade and replace oxidatively modified proteins at a higher rate compared to icefishes but rather, persist with higher levels of oxidized proteins.

Authors+Show Affiliations

Department of Biology, Georgia Southern University, Statesboro, GA 30458, United States.Department of Biology, Valdosta State University, Valdosta, GA 31698, United States.Institute of Arctic Biology, University of Alaska, Fairbanks, Fairbanks, AK 99775, United States. Electronic address: kmobrien@alaska.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26051614

Citation

Lewis, Johanne M., et al. "Energetic Costs of Protein Synthesis Do Not Differ Between Red- and White-blooded Antarctic Notothenioid Fishes." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 187, 2015, pp. 177-83.
Lewis JM, Grove TJ, O'Brien KM. Energetic costs of protein synthesis do not differ between red- and white-blooded Antarctic notothenioid fishes. Comp Biochem Physiol A Mol Integr Physiol. 2015;187:177-83.
Lewis, J. M., Grove, T. J., & O'Brien, K. M. (2015). Energetic costs of protein synthesis do not differ between red- and white-blooded Antarctic notothenioid fishes. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 187, 177-83. https://doi.org/10.1016/j.cbpa.2015.05.026
Lewis JM, Grove TJ, O'Brien KM. Energetic Costs of Protein Synthesis Do Not Differ Between Red- and White-blooded Antarctic Notothenioid Fishes. Comp Biochem Physiol A Mol Integr Physiol. 2015;187:177-83. PubMed PMID: 26051614.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Energetic costs of protein synthesis do not differ between red- and white-blooded Antarctic notothenioid fishes. AU - Lewis,Johanne M, AU - Grove,Theresa J, AU - O'Brien,Kristin M, Y1 - 2015/06/05/ PY - 2015/03/10/received PY - 2015/05/26/revised PY - 2015/05/29/accepted PY - 2015/6/9/entrez PY - 2015/6/9/pubmed PY - 2016/3/22/medline KW - Antarctic notothenioids KW - Oxygen consumption rates KW - Protein oxidation KW - Protein synthesis SP - 177 EP - 83 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 187 N2 - Antarctic icefishes (Family Channichthyidae) within the suborder Notothenioidei lack the oxygen-binding protein hemoglobin (Hb), and six of the 16 species of icefishes lack myoglobin (Mb) in heart ventricle. As iron-centered proteins, Hb and Mb can promote the formation of reactive oxygen species (ROS) that damage biological macromolecules. Consistent with this, our previous studies have shown that icefishes have lower levels of oxidized proteins and lipids in oxidative muscle compared to red-blooded notothenioids. Because oxidized proteins are usually degraded by the 20S proteasome and must be resynthesized, we hypothesized that rates of protein synthesis would be lower in icefishes compared to red-blooded notothenioids, thereby reducing the energetic costs of protein synthesis and conferring a benefit to the loss of Hb and Mb. Rates of protein synthesis were quantified in hearts, and the fraction of oxygen consumption devoted to protein synthesis was measured in isolated hepatocytes and cardiomyocytes of notothenioids differing in the expression of Hb and cardiac Mb. Neither rates of protein synthesis nor the energetic costs of protein synthesis differed among species, suggesting that red-blooded species do not degrade and replace oxidatively modified proteins at a higher rate compared to icefishes but rather, persist with higher levels of oxidized proteins. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/26051614/Energetic_costs_of_protein_synthesis_do_not_differ_between_red__and_white_blooded_Antarctic_notothenioid_fishes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(15)00161-0 DB - PRIME DP - Unbound Medicine ER -
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