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[Effect of moxibustion with ignited Zhuang-medicine medicated-thread on interstitial cells of Cajal in gastric antrum in diabetic gastroparesis rats].
Zhen Ci Yan Jiu. 2015 Apr; 40(2):104-7, 118.ZC

Abstract

OBJECTIVE

To observe the effect of Zhuang-medicine medicated-thread moxibustion therapy on interstitial cells of Cajal (ICC) in the gastric antrum of diabetic gastroparesis (DGP) rats, so as to investigate its mechanism underlying improving DGP.

METHODS

Sprague-Dawley (SD) male rats were randomly divided into 3 groups: control (n = 30) , model (n = 30) and moxibustion (n = 30). The DGP model was established by intraperitoneal injection of streptozotocin (STZ) and by feeding the rats with high fat-sugar forage. Zhuang-medicine medicated-thread moxibustion was applied to "Zhongwan" (CV 12), bilateral "Pishu" (BL 20), "Weishu" (BL 21), "Neiguan" (PC 6) and "Zusanli" (ST 36) once per day, for 3 weeks except weekends. The gastrointestinal propulsion rate and weight of stool in 24 h were determined, and c-kit (a marker for ICC) expression of the gastric antrum tissue was measured by immunohistochemistry.

RESULTS

The stool weight was significantly higher in the model group than in the control group (P < 0.01), but the rate of gastrointestinal propulsion and the rate of c-kit immunoreaction (IR) positive cells in the gastric antrum tissue were significantly lower in the model group than in the control group (P < 0.01). After moxibustion, the increased stool weight and the decreased gastrointestinal propulsion rate and decreased c-kit IR-positive cell rate were reversed (P < 0.01).

CONCLUSION

Zhuang-medicine medicated-thread moxibustion therapy can improve gastrointestinal function in DGP rats, which may be associated with its effect in up-regulating the expression of c-kit IR-positive ICC.

Authors

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Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26054193

Citation

Zhang, Hui, et al. "[Effect of Moxibustion With Ignited Zhuang-medicine Medicated-thread On Interstitial Cells of Cajal in Gastric Antrum in Diabetic Gastroparesis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 2, 2015, pp. 104-7, 118.
Zhang H, Miao FR, Luo YY, et al. [Effect of moxibustion with ignited Zhuang-medicine medicated-thread on interstitial cells of Cajal in gastric antrum in diabetic gastroparesis rats]. Zhen Ci Yan Jiu. 2015;40(2):104-7, 118.
Zhang, H., Miao, F. R., Luo, Y. Y., Liu, J. L., & Fan, Y. S. (2015). [Effect of moxibustion with ignited Zhuang-medicine medicated-thread on interstitial cells of Cajal in gastric antrum in diabetic gastroparesis rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(2), 104-7, 118.
Zhang H, et al. [Effect of Moxibustion With Ignited Zhuang-medicine Medicated-thread On Interstitial Cells of Cajal in Gastric Antrum in Diabetic Gastroparesis Rats]. Zhen Ci Yan Jiu. 2015;40(2):104-7, 118. PubMed PMID: 26054193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of moxibustion with ignited Zhuang-medicine medicated-thread on interstitial cells of Cajal in gastric antrum in diabetic gastroparesis rats]. AU - Zhang,Hui, AU - Miao,Fu-Rui, AU - Luo,Yu-Ying, AU - Liu,Jun-Ling, AU - Fan,Yu-Shan, PY - 2015/6/10/entrez PY - 2015/6/10/pubmed PY - 2015/7/3/medline SP - 104-7, 118 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 2 N2 - OBJECTIVE: To observe the effect of Zhuang-medicine medicated-thread moxibustion therapy on interstitial cells of Cajal (ICC) in the gastric antrum of diabetic gastroparesis (DGP) rats, so as to investigate its mechanism underlying improving DGP. METHODS: Sprague-Dawley (SD) male rats were randomly divided into 3 groups: control (n = 30) , model (n = 30) and moxibustion (n = 30). The DGP model was established by intraperitoneal injection of streptozotocin (STZ) and by feeding the rats with high fat-sugar forage. Zhuang-medicine medicated-thread moxibustion was applied to "Zhongwan" (CV 12), bilateral "Pishu" (BL 20), "Weishu" (BL 21), "Neiguan" (PC 6) and "Zusanli" (ST 36) once per day, for 3 weeks except weekends. The gastrointestinal propulsion rate and weight of stool in 24 h were determined, and c-kit (a marker for ICC) expression of the gastric antrum tissue was measured by immunohistochemistry. RESULTS: The stool weight was significantly higher in the model group than in the control group (P < 0.01), but the rate of gastrointestinal propulsion and the rate of c-kit immunoreaction (IR) positive cells in the gastric antrum tissue were significantly lower in the model group than in the control group (P < 0.01). After moxibustion, the increased stool weight and the decreased gastrointestinal propulsion rate and decreased c-kit IR-positive cell rate were reversed (P < 0.01). CONCLUSION: Zhuang-medicine medicated-thread moxibustion therapy can improve gastrointestinal function in DGP rats, which may be associated with its effect in up-regulating the expression of c-kit IR-positive ICC. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26054193/[Effect_of_moxibustion_with_ignited_Zhuang_medicine_medicated_thread_on_interstitial_cells_of_Cajal_in_gastric_antrum_in_diabetic_gastroparesis_rats]_ DB - PRIME DP - Unbound Medicine ER -