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[Effect of electroacupunctu-e intervention on serum IL-17 and IL-23 contents in rheumatoid arthritis rats].
Zhen Ci Yan Jiu. 2015 Apr; 40(2):141-5.ZC

Abstract

OBJECTIVE

To observe the influence of electroacupuncture (EA) stimulation of "Zusanli" (ST 36) and "Kunlun" (BL 60) on serum and knee-joint IL-17 and IL-23 contents in rheumatoid arthritis (RA) rats so as to study its underlying mechanism in improving RA.

METHODS

Male Wistar rats were randomly allocated to normal control, RA model, EA and Prednisolone groups (n = 8 in each group). The RA model was established by intra-planta injection of incomplete Freund's adjuvant and type II collagen. EA (2 Hz/100 Hz,1-2 mA)was applied to bilateral "Zusanli"(ST 36) and "Kunlun"(BL 60) for 30 min, once daily for 10 days. The rats' ankle diameter was measured, and IL-17 and IL-23 contents in the serum and the knee-joint cavity were assayed by ELISA and Western blot, respectively.

RESULTS

In comparison with the normal control group, the rats' ankle diameter, serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were significantly increased in the model group (P < 0.05). After EA and Prednisolone treatment, compared with the model group, all the rats' ankle diameter, serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were decreased remarkably (P < 0.05). No obvious differences were found between the EA and Prednisolone groups in the aforementioned indexes (P > 0.05), except IL-17 protein expression level (being markedly lower in the Prednisolone group than in the EA group).

CONCLUSION

EA intervention can reduce inflammatory reaction of the ankle-joint in RA rats, which may be related to its effects in down-regulating serum and knee-joint IL-17 and IL-23 levels.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

26054200

Citation

Guo, Li-Hong, et al. "[Effect of Electroacupunctu-e Intervention On Serum IL-17 and IL-23 Contents in Rheumatoid Arthritis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 40, no. 2, 2015, pp. 141-5.
Guo LH, Liu CX, Tian GH, et al. [Effect of electroacupunctu-e intervention on serum IL-17 and IL-23 contents in rheumatoid arthritis rats]. Zhen Ci Yan Jiu. 2015;40(2):141-5.
Guo, L. H., Liu, C. X., Tian, G. H., Ren, L. N., Zhang, R., Yin, Y., Zhou, M. L., & Ma, W. Z. (2015). [Effect of electroacupunctu-e intervention on serum IL-17 and IL-23 contents in rheumatoid arthritis rats]. Zhen Ci Yan Jiu = Acupuncture Research, 40(2), 141-5.
Guo LH, et al. [Effect of Electroacupunctu-e Intervention On Serum IL-17 and IL-23 Contents in Rheumatoid Arthritis Rats]. Zhen Ci Yan Jiu. 2015;40(2):141-5. PubMed PMID: 26054200.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of electroacupunctu-e intervention on serum IL-17 and IL-23 contents in rheumatoid arthritis rats]. AU - Guo,Li-Hong, AU - Liu,Chang-Xin, AU - Tian,Gui-Hua, AU - Ren,Li-Na, AU - Zhang,Rui, AU - Yin,Yuan, AU - Zhou,Ming-Liang, AU - Ma,Wen-Zhu, PY - 2015/6/10/entrez PY - 2015/6/10/pubmed PY - 2015/7/3/medline SP - 141 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 40 IS - 2 N2 - OBJECTIVE: To observe the influence of electroacupuncture (EA) stimulation of "Zusanli" (ST 36) and "Kunlun" (BL 60) on serum and knee-joint IL-17 and IL-23 contents in rheumatoid arthritis (RA) rats so as to study its underlying mechanism in improving RA. METHODS: Male Wistar rats were randomly allocated to normal control, RA model, EA and Prednisolone groups (n = 8 in each group). The RA model was established by intra-planta injection of incomplete Freund's adjuvant and type II collagen. EA (2 Hz/100 Hz,1-2 mA)was applied to bilateral "Zusanli"(ST 36) and "Kunlun"(BL 60) for 30 min, once daily for 10 days. The rats' ankle diameter was measured, and IL-17 and IL-23 contents in the serum and the knee-joint cavity were assayed by ELISA and Western blot, respectively. RESULTS: In comparison with the normal control group, the rats' ankle diameter, serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were significantly increased in the model group (P < 0.05). After EA and Prednisolone treatment, compared with the model group, all the rats' ankle diameter, serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were decreased remarkably (P < 0.05). No obvious differences were found between the EA and Prednisolone groups in the aforementioned indexes (P > 0.05), except IL-17 protein expression level (being markedly lower in the Prednisolone group than in the EA group). CONCLUSION: EA intervention can reduce inflammatory reaction of the ankle-joint in RA rats, which may be related to its effects in down-regulating serum and knee-joint IL-17 and IL-23 levels. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/26054200/[Effect_of_electroacupunctu_e_intervention_on_serum_IL_17_and_IL_23_contents_in_rheumatoid_arthritis_rats]_ L2 - http://www.diseaseinfosearch.org/result/592 DB - PRIME DP - Unbound Medicine ER -