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Synergistic mutual potentiation of antifungal activity of Zuccagnia punctata Cav. and Larrea nitida Cav. extracts in clinical isolates of Candida albicans and Candida glabrata.
Phytomedicine. 2015 Jun 01; 22(6):666-78.P

Abstract

BACKGROUND

Zuccagnia punctata Cav. (Fabaceae) and Larrea nitida Cav. (Zygophyllaceae) are indistinctly or jointly used in traditional medicine for the treatment of fungal-related infections. Although their dichloromethane (DCM) extract have demonstrated moderate antifungal activities when tested on their own, antifungal properties of combinations of both plants have not been assessed previously.

PURPOSE

The aim of this study was to establish with statistical rigor whether Z. punctata (ZpE) and L. nitida DCM extract (LnE) interact synergistically against the clinically important fungi Candida albicans and Candida glabrata and to characterize the most synergistic combinations.

STUDY DESIGN

For synergism assessment, the statistical-based Boik's design was applied. Eight ZpE-LnE fixed-ratio mixtures were prepared from four different months of 1 year and tested against Candida strains. Lϕ (Loewe index) of each mixture at different fractions affected (ϕ) allowed for the finding of the most synergistic combinations, which were characterized by HPLC fingerprint and by the quantitation of the selected marker compounds.

METHODS

Lϕ and confidence intervals were determined in vitro with the MixLow method, once the estimated parameters from the dose-response curves of independent extracts and mixtures, were obtained. Markers (four flavonoids for ZpE and three lignans for LnE) were quantified in each extract and their combinations, with a valid HPLC-UV method. The 3D-HPLC profiles of the most synergistic mixtures were obtained by HPLC-DAD.

RESULTS

Three over four IC50ZpE/IC50LnE fixed-ratio mixtures displayed synergistic interactions at effect levels ϕ > 0.5 against C. albicans. The dosis of the most synergistic (Lϕ = 0.62) mixture was 65.96 µg/ml (ZpE = 28%; LnE = 72%) containing 8 and 36% of flavonoids and lignans respectively. On the other hand, one over four IC50ZpE/IC50LnE mixtures displays synergistic interactions at ϕ > 0.5 against C. glabrata. The dosis of the most synergistic (Lϕ = 0.67) mixture was 168.23 µg/ml (ZpE = 27%; LnE = 73%) with 9.7 and 31.6% of flavonoids and lignans respectively.

CONCLUSIONS

Studies with the statistical-based MixLow method, allowed for the finding of the most ZpE-LnE synergistic mixtures, giving support to a proper joint use of both antifungal herbs in traditional medicine.

Authors+Show Affiliations

Área Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.Área Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.Área Estadística, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina.Instituto de Biotecnología, Facultad de Ingeniería, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), 5400 San Juan, Argentina.Instituto de Biotecnología, Facultad de Ingeniería, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), 5400 San Juan, Argentina.Área Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina. Electronic address: szaabgil@citynet.net.ar.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26055132

Citation

Butassi, Estefanía, et al. "Synergistic Mutual Potentiation of Antifungal Activity of Zuccagnia Punctata Cav. and Larrea Nitida Cav. Extracts in Clinical Isolates of Candida Albicans and Candida Glabrata." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 22, no. 6, 2015, pp. 666-78.
Butassi E, Svetaz LA, Ivancovich JJ, et al. Synergistic mutual potentiation of antifungal activity of Zuccagnia punctata Cav. and Larrea nitida Cav. extracts in clinical isolates of Candida albicans and Candida glabrata. Phytomedicine. 2015;22(6):666-78.
Butassi, E., Svetaz, L. A., Ivancovich, J. J., Feresin, G. E., Tapia, A., & Zacchino, S. A. (2015). Synergistic mutual potentiation of antifungal activity of Zuccagnia punctata Cav. and Larrea nitida Cav. extracts in clinical isolates of Candida albicans and Candida glabrata. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 22(6), 666-78. https://doi.org/10.1016/j.phymed.2015.04.004
Butassi E, et al. Synergistic Mutual Potentiation of Antifungal Activity of Zuccagnia Punctata Cav. and Larrea Nitida Cav. Extracts in Clinical Isolates of Candida Albicans and Candida Glabrata. Phytomedicine. 2015 Jun 1;22(6):666-78. PubMed PMID: 26055132.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic mutual potentiation of antifungal activity of Zuccagnia punctata Cav. and Larrea nitida Cav. extracts in clinical isolates of Candida albicans and Candida glabrata. AU - Butassi,Estefanía, AU - Svetaz,Laura A, AU - Ivancovich,Juan J, AU - Feresin,Gabriela E, AU - Tapia,Alejandro, AU - Zacchino,Susana A, Y1 - 2015/05/15/ PY - 2015/04/08/received PY - 2015/04/27/revised PY - 2015/04/28/accepted PY - 2015/6/10/entrez PY - 2015/6/10/pubmed PY - 2015/9/12/medline KW - Antifungal KW - Bi-herbal combinations KW - Larrea nitida KW - MixLow method KW - Synergism KW - Zuccagnia punctata SP - 666 EP - 78 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 22 IS - 6 N2 - BACKGROUND: Zuccagnia punctata Cav. (Fabaceae) and Larrea nitida Cav. (Zygophyllaceae) are indistinctly or jointly used in traditional medicine for the treatment of fungal-related infections. Although their dichloromethane (DCM) extract have demonstrated moderate antifungal activities when tested on their own, antifungal properties of combinations of both plants have not been assessed previously. PURPOSE: The aim of this study was to establish with statistical rigor whether Z. punctata (ZpE) and L. nitida DCM extract (LnE) interact synergistically against the clinically important fungi Candida albicans and Candida glabrata and to characterize the most synergistic combinations. STUDY DESIGN: For synergism assessment, the statistical-based Boik's design was applied. Eight ZpE-LnE fixed-ratio mixtures were prepared from four different months of 1 year and tested against Candida strains. Lϕ (Loewe index) of each mixture at different fractions affected (ϕ) allowed for the finding of the most synergistic combinations, which were characterized by HPLC fingerprint and by the quantitation of the selected marker compounds. METHODS: Lϕ and confidence intervals were determined in vitro with the MixLow method, once the estimated parameters from the dose-response curves of independent extracts and mixtures, were obtained. Markers (four flavonoids for ZpE and three lignans for LnE) were quantified in each extract and their combinations, with a valid HPLC-UV method. The 3D-HPLC profiles of the most synergistic mixtures were obtained by HPLC-DAD. RESULTS: Three over four IC50ZpE/IC50LnE fixed-ratio mixtures displayed synergistic interactions at effect levels ϕ > 0.5 against C. albicans. The dosis of the most synergistic (Lϕ = 0.62) mixture was 65.96 µg/ml (ZpE = 28%; LnE = 72%) containing 8 and 36% of flavonoids and lignans respectively. On the other hand, one over four IC50ZpE/IC50LnE mixtures displays synergistic interactions at ϕ > 0.5 against C. glabrata. The dosis of the most synergistic (Lϕ = 0.67) mixture was 168.23 µg/ml (ZpE = 27%; LnE = 73%) with 9.7 and 31.6% of flavonoids and lignans respectively. CONCLUSIONS: Studies with the statistical-based MixLow method, allowed for the finding of the most ZpE-LnE synergistic mixtures, giving support to a proper joint use of both antifungal herbs in traditional medicine. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/26055132/Synergistic_mutual_potentiation_of_antifungal_activity_of_Zuccagnia_punctata_Cav__and_Larrea_nitida_Cav__extracts_in_clinical_isolates_of_Candida_albicans_and_Candida_glabrata_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(15)00109-9 DB - PRIME DP - Unbound Medicine ER -