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Sesamol suppresses the inflammatory response by inhibiting NF-κB/MAPK activation and upregulating AMP kinase signaling in RAW 264.7 macrophages.
Inflamm Res. 2015 Aug; 64(8):577-88.IR

Abstract

OBJECTIVES AND DESIGN

Sesamol is a lignan isolated from sesame seed oil. In recent years, it was found that sesamol could decrease lung inflammation and lipopolysaccharide (LPS)-induced lung injury in rats. In this study, we investigated whether sesamol exhibited anti-inflammatory activity in LPS-stimulated macrophages.

MATERIALS AND METHODS

RAW 264.7 cells were treated with sesamol, then treated with LPS to induce inflammation. The levels of proinflammatory cytokines were analyzed with ELISA. The gene and protein expression of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), and nuclear factor erythroid-2-related factor 2 (Nrf2) were evaluated with real-time PCR and Western blots, respectively. We also examined inflammatory signaling pathways, including nuclear transcription factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways.

RESULTS

Sesamol inhibited production of nitric oxide, prostaglandin E2 (PGE2), and proinflammatory cytokines. Sesamol markedly suppressed mRNA and protein expression of iNOS and COX-2. Sesamol enhanced the protective antioxidant pathway represented by Nrf2 and HO-1. Moreover, sesamol suppressed NF-κB transport into the nucleus and decreased MAPK activation, but it promoted adenosine monophosphate-activated protein kinase (AMPK) activation.

CONCLUSIONS

These data suggested that sesamol ameliorated inflammatory and oxidative damage by upregulating AMPK activation and Nrf2 signaling and blocking the NF-κB and MAPK signaling pathways.

Authors+Show Affiliations

Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, 261 Wen-Hwa 1st Road, Kwei-Shan, Tao-Yuan, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26059394

Citation

Wu, Xin-Ling, et al. "Sesamol Suppresses the Inflammatory Response By Inhibiting NF-κB/MAPK Activation and Upregulating AMP Kinase Signaling in RAW 264.7 Macrophages." Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], vol. 64, no. 8, 2015, pp. 577-88.
Wu XL, Liou CJ, Li ZY, et al. Sesamol suppresses the inflammatory response by inhibiting NF-κB/MAPK activation and upregulating AMP kinase signaling in RAW 264.7 macrophages. Inflamm Res. 2015;64(8):577-88.
Wu, X. L., Liou, C. J., Li, Z. Y., Lai, X. Y., Fang, L. W., & Huang, W. C. (2015). Sesamol suppresses the inflammatory response by inhibiting NF-κB/MAPK activation and upregulating AMP kinase signaling in RAW 264.7 macrophages. Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.], 64(8), 577-88. https://doi.org/10.1007/s00011-015-0836-7
Wu XL, et al. Sesamol Suppresses the Inflammatory Response By Inhibiting NF-κB/MAPK Activation and Upregulating AMP Kinase Signaling in RAW 264.7 Macrophages. Inflamm Res. 2015;64(8):577-88. PubMed PMID: 26059394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sesamol suppresses the inflammatory response by inhibiting NF-κB/MAPK activation and upregulating AMP kinase signaling in RAW 264.7 macrophages. AU - Wu,Xin-Ling, AU - Liou,Chian-Jiun, AU - Li,Zih-Ying, AU - Lai,Xuan-Yu, AU - Fang,Li-Wen, AU - Huang,Wen-Chung, Y1 - 2015/06/10/ PY - 2015/03/17/received PY - 2015/05/29/accepted PY - 2015/05/12/revised PY - 2015/6/11/entrez PY - 2015/6/11/pubmed PY - 2016/4/8/medline SP - 577 EP - 88 JF - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JO - Inflamm Res VL - 64 IS - 8 N2 - OBJECTIVES AND DESIGN: Sesamol is a lignan isolated from sesame seed oil. In recent years, it was found that sesamol could decrease lung inflammation and lipopolysaccharide (LPS)-induced lung injury in rats. In this study, we investigated whether sesamol exhibited anti-inflammatory activity in LPS-stimulated macrophages. MATERIALS AND METHODS: RAW 264.7 cells were treated with sesamol, then treated with LPS to induce inflammation. The levels of proinflammatory cytokines were analyzed with ELISA. The gene and protein expression of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), and nuclear factor erythroid-2-related factor 2 (Nrf2) were evaluated with real-time PCR and Western blots, respectively. We also examined inflammatory signaling pathways, including nuclear transcription factor kappa-B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. RESULTS: Sesamol inhibited production of nitric oxide, prostaglandin E2 (PGE2), and proinflammatory cytokines. Sesamol markedly suppressed mRNA and protein expression of iNOS and COX-2. Sesamol enhanced the protective antioxidant pathway represented by Nrf2 and HO-1. Moreover, sesamol suppressed NF-κB transport into the nucleus and decreased MAPK activation, but it promoted adenosine monophosphate-activated protein kinase (AMPK) activation. CONCLUSIONS: These data suggested that sesamol ameliorated inflammatory and oxidative damage by upregulating AMPK activation and Nrf2 signaling and blocking the NF-κB and MAPK signaling pathways. SN - 1420-908X UR - https://www.unboundmedicine.com/medline/citation/26059394/Sesamol_suppresses_the_inflammatory_response_by_inhibiting_NF_κB/MAPK_activation_and_upregulating_AMP_kinase_signaling_in_RAW_264_7_macrophages_ L2 - https://dx.doi.org/10.1007/s00011-015-0836-7 DB - PRIME DP - Unbound Medicine ER -