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Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover).
Biomed Res Int. 2015; 2015:643285.BR

Abstract

In the present study the extracts of in vivo and in vitro grown plants as well as callus tissue of red clover were tested for their antioxidant activities, using different extraction solvent and different antioxidant assays. The total flavonoid and phenolic contents as well as extraction yield of the extracts were also investigated to determine their correlation with the antioxidant activity of the extracts. Among all the tested extracts the highest amounts of total phenolic and total flavonoids content were found in methanol extract of in vivo grown plants. The antioxidant activity of tested samples followed the order in vivo plant extract > callus extract > in vitro extract. The highest reducing power, 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, and chelating power were found in methanol extracts of in vivo grown red clover, while the chloroform fraction of in vivo grown plants showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, superoxide anion radical scavenging and hydrogen peroxide scavenging compared to the other tested extracts. A significant correlation was found between the antioxidant activity of extracts and their total phenolic and total flavonoid content. According to the findings, the extract of in vitro culture of red clover especially the callus tissue possesses a comparable antioxidant activity to the in vivo cultured plants' extract.

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Authors+Show Affiliations

Khorasani Esmaeili A
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Mat Taha R
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Mohajer S
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Banisalam B
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.

MeSH

AntioxidantsBiphenyl CompoundsFlavonoidsFree Radical ScavengersLipid PeroxidationOxidation-ReductionPhenolsPicratesPlant ExtractsPlant LeavesSuperoxidesTrifolium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26064936

Citation

Khorasani Esmaeili, Arash, et al. "Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts From in Vivo and in Vitro Grown Trifolium Pratense L. (Red Clover)." BioMed Research International, vol. 2015, 2015, p. 643285.
Khorasani Esmaeili A, Mat Taha R, Mohajer S, et al. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). Biomed Res Int. 2015;2015:643285.
Khorasani Esmaeili, A., Mat Taha, R., Mohajer, S., & Banisalam, B. (2015). Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). BioMed Research International, 2015, 643285. https://doi.org/10.1155/2015/643285
Khorasani Esmaeili A, et al. Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts From in Vivo and in Vitro Grown Trifolium Pratense L. (Red Clover). Biomed Res Int. 2015;2015:643285. PubMed PMID: 26064936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts from In Vivo and In Vitro Grown Trifolium pratense L. (Red Clover). AU - Khorasani Esmaeili,Arash, AU - Mat Taha,Rosna, AU - Mohajer,Sadegh, AU - Banisalam,Behrooz, Y1 - 2015/05/06/ PY - 2015/01/20/received PY - 2015/04/02/revised PY - 2015/04/22/accepted PY - 2015/6/12/entrez PY - 2015/6/13/pubmed PY - 2016/3/11/medline SP - 643285 EP - 643285 JF - BioMed research international JO - Biomed Res Int VL - 2015 N2 - In the present study the extracts of in vivo and in vitro grown plants as well as callus tissue of red clover were tested for their antioxidant activities, using different extraction solvent and different antioxidant assays. The total flavonoid and phenolic contents as well as extraction yield of the extracts were also investigated to determine their correlation with the antioxidant activity of the extracts. Among all the tested extracts the highest amounts of total phenolic and total flavonoids content were found in methanol extract of in vivo grown plants. The antioxidant activity of tested samples followed the order in vivo plant extract > callus extract > in vitro extract. The highest reducing power, 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, and chelating power were found in methanol extracts of in vivo grown red clover, while the chloroform fraction of in vivo grown plants showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, superoxide anion radical scavenging and hydrogen peroxide scavenging compared to the other tested extracts. A significant correlation was found between the antioxidant activity of extracts and their total phenolic and total flavonoid content. According to the findings, the extract of in vitro culture of red clover especially the callus tissue possesses a comparable antioxidant activity to the in vivo cultured plants' extract. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/26064936/Antioxidant_Activity_and_Total_Phenolic_and_Flavonoid_Content_of_Various_Solvent_Extracts_from_In_Vivo_and_In_Vitro_Grown_Trifolium_pratense_L___Red_Clover__ DB - PRIME DP - Unbound Medicine ER -