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Clitocypin, a fungal cysteine protease inhibitor, exerts its insecticidal effect on Colorado potato beetle larvae by inhibiting their digestive cysteine proteases.
Pestic Biochem Physiol. 2015 Jul; 122:59-66.PB

Abstract

Colorado potato beetle (Leptinotarsa decemlineata Say, CPB) is a major potato pest that adapts readily to insecticides. Several types of protease inhibitors have previously been investigated as potential control agents, but with limited success. Recently, cysteine protease inhibitors from parasol mushroom, the macrocypins, were reported to inhibit growth of CPB larvae. To further investigate the insecticidal potential and mode of action of cysteine protease inhibitors of fungal origin, clitocypin, a cysteine protease inhibitor from clouded agaric (Clitocybe nebularis), was evaluated for its lethal effects on CPB larvae. Clitocypin isolated from fruiting bodies and recombinant clitocypin produced in Escherichia coli slowed growth and reduced survival of CPB larvae in a concentration dependent manner. Clitocypin was also expressed by transgenic potato, but only at low levels. Nevertheless, it reduced larval weight gain and delayed development. We have additionally shown that younger larvae are more susceptible to the action of clitocypin. The inhibition of digestive cysteine proteases, intestains, by clitocypin was shown to be the underlying mode of action. Protease inhibitors from mushrooms are confirmed as promising candidates for biopesticides.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Šmid I
Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, Ljubljana 1000, Slovenia.
Rotter A
Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, Ljubljana 1000, Slovenia.
Gruden K
Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, Ljubljana 1000, Slovenia; Department of Biotechnology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana 1000, Slovenia.
Brzin J
Department of Biotechnology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana 1000, Slovenia.
Buh Gašparič M
Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, Ljubljana 1000, Slovenia.
Kos J
Department of Biotechnology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana 1000, Slovenia; Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, Ljubljana 1000, Slovenia.
Žel J
Department of Biotechnology and Systems Biology, National Institute of Biology, Večna pot 111, Ljubljana 1000, Slovenia.
Sabotič J
Department of Biotechnology, Jožef Stefan Institute, Jamova cesta 39, Ljubljana 1000, Slovenia. Electronic address: jerica.sabotic@ijs.si.

MeSH

AnimalsColeopteraCysteine Proteinase InhibitorsDigestive SystemDose-Response Relationship, DrugFemaleFungal ProteinsGene Expression RegulationInsecticidesLarvaPlants, Genetically ModifiedRecombinant ProteinsSolanum tuberosum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26071808

Citation

Šmid, Ida, et al. "Clitocypin, a Fungal Cysteine Protease Inhibitor, Exerts Its Insecticidal Effect On Colorado Potato Beetle Larvae By Inhibiting Their Digestive Cysteine Proteases." Pesticide Biochemistry and Physiology, vol. 122, 2015, pp. 59-66.
Šmid I, Rotter A, Gruden K, et al. Clitocypin, a fungal cysteine protease inhibitor, exerts its insecticidal effect on Colorado potato beetle larvae by inhibiting their digestive cysteine proteases. Pestic Biochem Physiol. 2015;122:59-66.
Šmid, I., Rotter, A., Gruden, K., Brzin, J., Buh Gašparič, M., Kos, J., Žel, J., & Sabotič, J. (2015). Clitocypin, a fungal cysteine protease inhibitor, exerts its insecticidal effect on Colorado potato beetle larvae by inhibiting their digestive cysteine proteases. Pesticide Biochemistry and Physiology, 122, 59-66. https://doi.org/10.1016/j.pestbp.2014.12.022
Šmid I, et al. Clitocypin, a Fungal Cysteine Protease Inhibitor, Exerts Its Insecticidal Effect On Colorado Potato Beetle Larvae By Inhibiting Their Digestive Cysteine Proteases. Pestic Biochem Physiol. 2015;122:59-66. PubMed PMID: 26071808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clitocypin, a fungal cysteine protease inhibitor, exerts its insecticidal effect on Colorado potato beetle larvae by inhibiting their digestive cysteine proteases. AU - Šmid,Ida, AU - Rotter,Ana, AU - Gruden,Kristina, AU - Brzin,Jože, AU - Buh Gašparič,Meti, AU - Kos,Janko, AU - Žel,Jana, AU - Sabotič,Jerica, Y1 - 2014/12/23/ PY - 2014/07/28/received PY - 2014/11/07/revised PY - 2014/12/18/accepted PY - 2015/6/15/entrez PY - 2015/6/15/pubmed PY - 2016/3/25/medline KW - Bioinsecticide KW - Crop protection KW - Cysteine protease KW - Intestain KW - Protease inhibitor KW - Transgenic potato SP - 59 EP - 66 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 122 N2 - Colorado potato beetle (Leptinotarsa decemlineata Say, CPB) is a major potato pest that adapts readily to insecticides. Several types of protease inhibitors have previously been investigated as potential control agents, but with limited success. Recently, cysteine protease inhibitors from parasol mushroom, the macrocypins, were reported to inhibit growth of CPB larvae. To further investigate the insecticidal potential and mode of action of cysteine protease inhibitors of fungal origin, clitocypin, a cysteine protease inhibitor from clouded agaric (Clitocybe nebularis), was evaluated for its lethal effects on CPB larvae. Clitocypin isolated from fruiting bodies and recombinant clitocypin produced in Escherichia coli slowed growth and reduced survival of CPB larvae in a concentration dependent manner. Clitocypin was also expressed by transgenic potato, but only at low levels. Nevertheless, it reduced larval weight gain and delayed development. We have additionally shown that younger larvae are more susceptible to the action of clitocypin. The inhibition of digestive cysteine proteases, intestains, by clitocypin was shown to be the underlying mode of action. Protease inhibitors from mushrooms are confirmed as promising candidates for biopesticides. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/26071808/Clitocypin_a_fungal_cysteine_protease_inhibitor_exerts_its_insecticidal_effect_on_Colorado_potato_beetle_larvae_by_inhibiting_their_digestive_cysteine_proteases_ DB - PRIME DP - Unbound Medicine ER -