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Diabetes mellitus and the sodium electrochemical gradient across the brush border membrane of rat intestinal enterocytes.
J Endocrinol. 1989 Dec; 123(3):453-9.JE

Abstract

The effects of chronic diabetes mellitus of 4-5 weeks duration on the potential difference across the brush border membrane of rat small intestine (Vm) and on Na+-dependent uptake of D-glucose by jejunal brush border vesicles have been studied. Diabetes increased Vm in the jejunum from a mean value of -47.2 mV in control tissue to -57.4 mV in diabetic tissue (P less than 0.001) but was without effect on ileal Vm. Measurements of Vm during ion-substitution experiments revealed that the conductance of Na+ of the jejunal brush border was reduced by diabetes, whilst K+ and Cl- permeabilities were unaltered. Uptake studies using brush border vesicles and an Na+-electrochemical gradient showed that diabetes caused a 56% increase in the initial rate of uptake of D-glucose but was without effect on the peak to equilibrium ratio. Taken together, data from these two studies suggest that the lower Na+ permeability of the brush border in diabetes enhances the electrical and chemical driving force for active Na+-dependent uptake of glucose by reducing glucose-independent movement of Na+ across this membrane. Finally, the possible humoral factors involved in this response to diabetes are discussed.

Authors+Show Affiliations

Department of Physiology, Royal Free Hospital School of Medicine, London.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2607255

Citation

Debnam, E S., and H Y. Ebrahim. "Diabetes Mellitus and the Sodium Electrochemical Gradient Across the Brush Border Membrane of Rat Intestinal Enterocytes." The Journal of Endocrinology, vol. 123, no. 3, 1989, pp. 453-9.
Debnam ES, Ebrahim HY. Diabetes mellitus and the sodium electrochemical gradient across the brush border membrane of rat intestinal enterocytes. J Endocrinol. 1989;123(3):453-9.
Debnam, E. S., & Ebrahim, H. Y. (1989). Diabetes mellitus and the sodium electrochemical gradient across the brush border membrane of rat intestinal enterocytes. The Journal of Endocrinology, 123(3), 453-9.
Debnam ES, Ebrahim HY. Diabetes Mellitus and the Sodium Electrochemical Gradient Across the Brush Border Membrane of Rat Intestinal Enterocytes. J Endocrinol. 1989;123(3):453-9. PubMed PMID: 2607255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diabetes mellitus and the sodium electrochemical gradient across the brush border membrane of rat intestinal enterocytes. AU - Debnam,E S, AU - Ebrahim,H Y, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 453 EP - 9 JF - The Journal of endocrinology JO - J Endocrinol VL - 123 IS - 3 N2 - The effects of chronic diabetes mellitus of 4-5 weeks duration on the potential difference across the brush border membrane of rat small intestine (Vm) and on Na+-dependent uptake of D-glucose by jejunal brush border vesicles have been studied. Diabetes increased Vm in the jejunum from a mean value of -47.2 mV in control tissue to -57.4 mV in diabetic tissue (P less than 0.001) but was without effect on ileal Vm. Measurements of Vm during ion-substitution experiments revealed that the conductance of Na+ of the jejunal brush border was reduced by diabetes, whilst K+ and Cl- permeabilities were unaltered. Uptake studies using brush border vesicles and an Na+-electrochemical gradient showed that diabetes caused a 56% increase in the initial rate of uptake of D-glucose but was without effect on the peak to equilibrium ratio. Taken together, data from these two studies suggest that the lower Na+ permeability of the brush border in diabetes enhances the electrical and chemical driving force for active Na+-dependent uptake of glucose by reducing glucose-independent movement of Na+ across this membrane. Finally, the possible humoral factors involved in this response to diabetes are discussed. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/2607255/Diabetes_mellitus_and_the_sodium_electrochemical_gradient_across_the_brush_border_membrane_of_rat_intestinal_enterocytes_ L2 - https://joe.bioscientifica.com/doi/10.1677/joe.0.1230453 DB - PRIME DP - Unbound Medicine ER -