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YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells.
Chin J Nat Med. 2015 Jun; 13(6):438-44.CJ

Abstract

In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells and the underlying mechanisms. PC12 cells were exposed to 100 μmol·L(-1) of H2O2 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT (3, (4, 5-dimethylthiazole-2-yl) 2, 5-diphenyl-tetrazolium bromide) and lactate dehydrogenase (LDH) release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine123; and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by externalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders.

Authors+Show Affiliations

Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China.Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: honghao@cpu.edu.cn.Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China. Electronic address: fengsunlight@163.com.New Technology Center of Chemical Pharmaceutical Shanghai Institute of Pharmaceutical Industry China State Institute of Pharmaceutical Industry, Shanghai 200437, China.Jiangxi Qingfeng Pharmaceutical Corporation, Ganzhou 341000, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26073340

Citation

Zhao, Yu-Rong, et al. "YGS40, an Active Fraction of Yi-Gan San, Reduces Hydrogen Peroxide-induced Apoptosis in PC12 Cells." Chinese Journal of Natural Medicines, vol. 13, no. 6, 2015, pp. 438-44.
Zhao YR, Qu W, Liu WY, et al. YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells. Chin J Nat Med. 2015;13(6):438-44.
Zhao, Y. R., Qu, W., Liu, W. Y., Hong, H., Feng, F., Chen, H., & Xie, N. (2015). YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells. Chinese Journal of Natural Medicines, 13(6), 438-44. https://doi.org/10.1016/S1875-5364(15)30037-6
Zhao YR, et al. YGS40, an Active Fraction of Yi-Gan San, Reduces Hydrogen Peroxide-induced Apoptosis in PC12 Cells. Chin J Nat Med. 2015;13(6):438-44. PubMed PMID: 26073340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells. AU - Zhao,Yu-Rong, AU - Qu,Wei, AU - Liu,Wen-Yuan, AU - Hong,Hao, AU - Feng,Feng, AU - Chen,Han, AU - Xie,Ning, PY - 2014/01/10/received PY - 2015/6/16/entrez PY - 2015/6/16/pubmed PY - 2016/3/2/medline KW - Antioxidant effects KW - Apoptosis KW - Hydrogen peroxide KW - YGS40 KW - Yi-Gan San SP - 438 EP - 44 JF - Chinese journal of natural medicines JO - Chin J Nat Med VL - 13 IS - 6 N2 - In our previous study, we have elucidated the chemical profile of YGS40, a fraction of Yi-Gan San (YGS), used for the treatment of Alzheimer's disease (AD). Oxidative stress-induced apoptosis is implicated in neurodegenerative disorders such as AD. The aim of the present study was to explore the protective effects of YGS40 against hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells and the underlying mechanisms. PC12 cells were exposed to 100 μmol·L(-1) of H2O2 for 12 h with or without YGS40 pretreatment. Cytotoxicity was determined by MTT (3, (4, 5-dimethylthiazole-2-yl) 2, 5-diphenyl-tetrazolium bromide) and lactate dehydrogenase (LDH) release assays; apoptosis was detected by Annexin V/propidium iodide coupled staining and by determining caspase-3 activity and Bax and Bcl-2 protein levels. Mitochondrial membrane potential (MMP) was assessed by the retention of rhodamine123; and the activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured using commercially available enzymatic kits. Pretreatment with YGS40 significantly prevented H2O2-induced cytotoxicity and protected the cells against H2O2-triggered apoptosis characterized by externalization of membrane phosphatidylserine and caspase-3 activation and the increased ratio of Bax/Bcl-2 in PC12 cells. Further studies showed that YGS40 suppressed H2O2-induced MMP loss, increased SOD activity, and decreased MDA level. These findings suggest that YGS40 may be beneficial for the prevention and treatment of oxidative stress-mediated disorders. SN - 1875-5364 UR - https://www.unboundmedicine.com/medline/citation/26073340/YGS40_an_active_fraction_of_Yi_Gan_San_reduces_hydrogen_peroxide_induced_apoptosis_in_PC12_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1875-5364(15)30037-6 DB - PRIME DP - Unbound Medicine ER -