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Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil.
Epidemiol Infect 2016; 144(1):72-5EI

Abstract

The aim of this study was to identify Leptospira in urine samples of cattle by direct sequencing of the secY gene. The validity of this approach was assessed using ten Leptospira strains obtained from cattle in Brazil and 77 DNA samples previously extracted from cattle urine, that were positive by PCR for the genus-specific lipL32 gene of Leptospira. Direct sequencing identified 24 (31·1%) interpretable secY sequences and these were identical to those obtained from direct DNA sequencing of the urine samples from which they were recovered. Phylogenetic analyses identified four species: L. interrogans, L. borgpetersenii, L. noguchii, and L. santarosai with the most prevalent genotypes being associated with L. borgpetersenii. While direct sequencing cannot, as yet, replace culturing of leptospires, it is a valid additional tool for epidemiological studies. An unexpected finding from this study was the genetic diversity of Leptospira infecting Brazilian cattle.

Authors+Show Affiliations

Laboratory of Veterinary Bacteriology,Department of Microbiology and Parasitology,Universidade Federal Fluminense,Niterói,RJ,Brazil.Bio-Manguinhos,Oswaldo Cruz Foundation,Brazilian Ministry of Health,Rio de Janeiro,RJ,Brazil.Bio-Manguinhos,Oswaldo Cruz Foundation,Brazilian Ministry of Health,Rio de Janeiro,RJ,Brazil.Laboratory of Veterinary Bacteriology,Department of Microbiology and Parasitology,Universidade Federal Fluminense,Niterói,RJ,Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26076668

Citation

Hamond, C, et al. "Genotyping of Leptospira Directly in Urine Samples of Cattle Demonstrates a Diversity of Species and Strains in Brazil." Epidemiology and Infection, vol. 144, no. 1, 2016, pp. 72-5.
Hamond C, Pestana CP, Medeiros MA, et al. Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil. Epidemiol Infect. 2016;144(1):72-5.
Hamond, C., Pestana, C. P., Medeiros, M. A., & Lilenbaum, W. (2016). Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil. Epidemiology and Infection, 144(1), pp. 72-5. doi:10.1017/S0950268815001363.
Hamond C, et al. Genotyping of Leptospira Directly in Urine Samples of Cattle Demonstrates a Diversity of Species and Strains in Brazil. Epidemiol Infect. 2016;144(1):72-5. PubMed PMID: 26076668.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotyping of Leptospira directly in urine samples of cattle demonstrates a diversity of species and strains in Brazil. AU - Hamond,C, AU - Pestana,C P, AU - Medeiros,M A, AU - Lilenbaum,W, Y1 - 2015/06/16/ PY - 2015/6/17/entrez PY - 2015/6/17/pubmed PY - 2016/3/15/medline KW - Leptospira KW - secY KW - urine sample SP - 72 EP - 5 JF - Epidemiology and infection JO - Epidemiol. Infect. VL - 144 IS - 1 N2 - The aim of this study was to identify Leptospira in urine samples of cattle by direct sequencing of the secY gene. The validity of this approach was assessed using ten Leptospira strains obtained from cattle in Brazil and 77 DNA samples previously extracted from cattle urine, that were positive by PCR for the genus-specific lipL32 gene of Leptospira. Direct sequencing identified 24 (31·1%) interpretable secY sequences and these were identical to those obtained from direct DNA sequencing of the urine samples from which they were recovered. Phylogenetic analyses identified four species: L. interrogans, L. borgpetersenii, L. noguchii, and L. santarosai with the most prevalent genotypes being associated with L. borgpetersenii. While direct sequencing cannot, as yet, replace culturing of leptospires, it is a valid additional tool for epidemiological studies. An unexpected finding from this study was the genetic diversity of Leptospira infecting Brazilian cattle. SN - 1469-4409 UR - https://www.unboundmedicine.com/medline/citation/26076668/Genotyping_of_Leptospira_directly_in_urine_samples_of_cattle_demonstrates_a_diversity_of_species_and_strains_in_Brazil_ L2 - https://www.cambridge.org/core/product/identifier/S0950268815001363/type/journal_article DB - PRIME DP - Unbound Medicine ER -