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Association between extracellular signal-regulated kinase expression and the anti-allodynic effect in rats with spared nerve injury by applying immediate pulsed radiofrequency.
BMC Anesthesiol. 2015 Jun 16; 15:92.BA

Abstract

BACKGROUND

The application of pulsed radiofrequency (PRF) close to the dorsal root ganglia, or peripheral nerves, has been demonstrated to be effective for the treatment of chronic neuropathic pain conditions. The goal of this study was to investigate the analgesic effect of immediate PRF treatment after nerve injury and its possible cellular alterations in the dorsal horn of the spinal cord in rats with spared nerve injury (SNI).

METHODS

Neuropathic pain was achieved in a SNI neuropathic pain model by ligating and cutting the common peroneal and tibial branches of the left sciatic nerve, leaving the sural nerve intact. Wistar rats were divided into four groups that received different treatments, i.e., SNI and PRF for 6 min at 45 V (SNI + PRF-45 V), at 60 V (SNI + PRF-60 V), SNI alone, and sham groups. After the SNI surgery, each rat was immediately given the PRF treatment (500 kHz, rate of 2 Hz, 20 ms duration, temperature below 42 °C) on the left sciatic nerve 0.3-0.4 cm proximal to the injured site. The behavioral measurements included mechanical allodynia and cold allodynia of the ipsilateral hind paw and were performed during the 28 days that followed the SNI surgery and PRF treatment. Total extracellular signal-regulated kinase 1 and 2 (ERK1/2) and phospho-ERK1/2 were measured using Western blot in the ipsilateral spinal cord from animals in the different groups.

RESULTS

The three groups of rats with nerve injuries manifested a lower paw withdrawal threshold (PWT) in the behavioral measurement of mechanical allodynia and a shorter painful-behavior duration in the cold allodynia test over 28 days. Mechanical allodynia measurement showed that both the PRF-45 V and PRF-60 V treatment groups exhibited a more prominent antiallodynic effect than did the SNI group from days 1 to 28 after surgery. Similarly, in comparison with the SNI group, both the SNI + PRF-45 V and SNI + PRF-60 V groups had significant inhibition on the cold allodynia measurement from days 1 to 28 after surgery. Furthermore, the activation of the extracellular signal-regulated kinase 1 and 2 (ERK1/2) in the ipsilateral spinal dorsal horn of SNI rats was effectively inhibited in the SNI + PRF-45 V and SNI + PRF-60 V groups for 28 days after surgery.

CONCLUSIONS

Immediate PRF application on the proximal nerve injury site provided a significant inhibition of neuropathic pain formation, accompanied by the inhibition of ERK activation.

Authors+Show Affiliations

School of Medicine, Fu Jen Catholic University, New Taipei, 24205, Taiwan. Department of Anesthesiology & Integrated Pain Management Center, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan. Department of Chemistry, Fu-Jen Catholic University and Graduate Institute of Basic Medicine, Fu-Jen Catholic University, Taipei, Taiwan.Department of Anesthesiology & Integrated Pain Management Center, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan.Department of Biochemical Science and Technology, National Chiayi University, Chiayi, Taiwan.Department of Anesthesiology & Integrated Pain Management Center, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan. Department of Anesthesiology, Cathay General Hospital, Taipei, Taiwan.School of Medicine, Fu Jen Catholic University, New Taipei, 24205, Taiwan. Department of Medical Research, Cathay General Hospital, Taipei, Taiwan. Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan.School of Medicine, Fu Jen Catholic University, New Taipei, 24205, Taiwan. 034407@mail.fju.edu.tw.School of Medicine, Fu Jen Catholic University, New Taipei, 24205, Taiwan. chiencmail@gmail.com. Department of Anesthesiology, Sijhih Cathay General Hospital, New Taipei, Taiwan. chiencmail@gmail.com. Department of Anesthesiology, Cathay General Hospital, Taipei, Taiwan. chiencmail@gmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26077473

Citation

Yeh, Chun-Chang, et al. "Association Between Extracellular Signal-regulated Kinase Expression and the Anti-allodynic Effect in Rats With Spared Nerve Injury By Applying Immediate Pulsed Radiofrequency." BMC Anesthesiology, vol. 15, 2015, p. 92.
Yeh CC, Wu ZF, Chen JC, et al. Association between extracellular signal-regulated kinase expression and the anti-allodynic effect in rats with spared nerve injury by applying immediate pulsed radiofrequency. BMC Anesthesiol. 2015;15:92.
Yeh, C. C., Wu, Z. F., Chen, J. C., Wong, C. S., Huang, C. J., Wang, J. S., & Chien, C. C. (2015). Association between extracellular signal-regulated kinase expression and the anti-allodynic effect in rats with spared nerve injury by applying immediate pulsed radiofrequency. BMC Anesthesiology, 15, 92. https://doi.org/10.1186/s12871-015-0071-3
Yeh CC, et al. Association Between Extracellular Signal-regulated Kinase Expression and the Anti-allodynic Effect in Rats With Spared Nerve Injury By Applying Immediate Pulsed Radiofrequency. BMC Anesthesiol. 2015 Jun 16;15:92. PubMed PMID: 26077473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between extracellular signal-regulated kinase expression and the anti-allodynic effect in rats with spared nerve injury by applying immediate pulsed radiofrequency. AU - Yeh,Chun-Chang, AU - Wu,Zhi-Fu, AU - Chen,Jui-Chieh, AU - Wong,Chih-Shung, AU - Huang,Chi-Jung, AU - Wang,Jinn-Shyan, AU - Chien,Chih-Cheng, Y1 - 2015/06/16/ PY - 2014/12/28/received PY - 2015/05/29/accepted PY - 2015/6/17/entrez PY - 2015/6/17/pubmed PY - 2016/1/12/medline SP - 92 EP - 92 JF - BMC anesthesiology JO - BMC Anesthesiol VL - 15 N2 - BACKGROUND: The application of pulsed radiofrequency (PRF) close to the dorsal root ganglia, or peripheral nerves, has been demonstrated to be effective for the treatment of chronic neuropathic pain conditions. The goal of this study was to investigate the analgesic effect of immediate PRF treatment after nerve injury and its possible cellular alterations in the dorsal horn of the spinal cord in rats with spared nerve injury (SNI). METHODS: Neuropathic pain was achieved in a SNI neuropathic pain model by ligating and cutting the common peroneal and tibial branches of the left sciatic nerve, leaving the sural nerve intact. Wistar rats were divided into four groups that received different treatments, i.e., SNI and PRF for 6 min at 45 V (SNI + PRF-45 V), at 60 V (SNI + PRF-60 V), SNI alone, and sham groups. After the SNI surgery, each rat was immediately given the PRF treatment (500 kHz, rate of 2 Hz, 20 ms duration, temperature below 42 °C) on the left sciatic nerve 0.3-0.4 cm proximal to the injured site. The behavioral measurements included mechanical allodynia and cold allodynia of the ipsilateral hind paw and were performed during the 28 days that followed the SNI surgery and PRF treatment. Total extracellular signal-regulated kinase 1 and 2 (ERK1/2) and phospho-ERK1/2 were measured using Western blot in the ipsilateral spinal cord from animals in the different groups. RESULTS: The three groups of rats with nerve injuries manifested a lower paw withdrawal threshold (PWT) in the behavioral measurement of mechanical allodynia and a shorter painful-behavior duration in the cold allodynia test over 28 days. Mechanical allodynia measurement showed that both the PRF-45 V and PRF-60 V treatment groups exhibited a more prominent antiallodynic effect than did the SNI group from days 1 to 28 after surgery. Similarly, in comparison with the SNI group, both the SNI + PRF-45 V and SNI + PRF-60 V groups had significant inhibition on the cold allodynia measurement from days 1 to 28 after surgery. Furthermore, the activation of the extracellular signal-regulated kinase 1 and 2 (ERK1/2) in the ipsilateral spinal dorsal horn of SNI rats was effectively inhibited in the SNI + PRF-45 V and SNI + PRF-60 V groups for 28 days after surgery. CONCLUSIONS: Immediate PRF application on the proximal nerve injury site provided a significant inhibition of neuropathic pain formation, accompanied by the inhibition of ERK activation. SN - 1471-2253 UR - https://www.unboundmedicine.com/medline/citation/26077473/Association_between_extracellular_signal_regulated_kinase_expression_and_the_anti_allodynic_effect_in_rats_with_spared_nerve_injury_by_applying_immediate_pulsed_radiofrequency_ L2 - https://bmcanesthesiol.biomedcentral.com/articles/10.1186/s12871-015-0071-3 DB - PRIME DP - Unbound Medicine ER -