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Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens.
New Phytol 2015; 208(3):846-59NP

Abstract

WRKY proteins are transcription factors (TFs) that regulate the expression of defense-related genes. The salicylic acid (SA)-inducible Oryza sativa WRKY6 (OsWRKY6) was identified as a positive regulator of Oryza sativa pathogenesis-related 10a (OsPR10a) by transient expression assays. A physical interaction between OsWRKY6 and W-box-like element 1 (WLE1), which positively regulates OsPR10a/probenazole induced protein 1 expression, was verified in vitro. Several pathogenesis-related (PR) genes were constitutively activated, including OsPR10a, and transgenic rice (Oryza sativa) plants overexpressing (ox) OsWRKY6 exhibited enhanced disease resistance to pathogens. By contrast, PR gene induction was compromised in transgenic OsWRKY6-RNAi lines, suggesting that OsWRKY6 is a positive regulator of defense responses. OsWRKY6-ox lines displayed leaf lesions, and increased OsWRKY6 levels caused cell death. Salicylic acid (SA) concentrations were higher in OsWRKY6-ox lines than in wild-type (WT) plants, and transcript levels of Oryza sativa isochorismate synthase 1 (OsICS1), which encodes a major enzyme involved in SA biosynthesis, were higher in OsWRKY6-ox lines than in WT. OsWRKY6 directly bound to the OsICS1 promoter in vivo. This indicates that OsWRKY6 can directly regulate OsICS1 expression and thereby increase SA concentrations. OsWRKY6 autoregulates its own expression. OsWRKY6 protein degradation is possibly regulated by ubiquitination. Our results suggest that OsWRKY6 positively regulates defense responses through activation of OsICS1 expression and OsWRKY6 stabilization.

Authors+Show Affiliations

National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.National Academy of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26083148

Citation

Choi, Changhyun, et al. "Molecular Characterization of Oryza Sativa WRKY6, Which Binds to W-box-like Element 1 of the Oryza Sativa Pathogenesis-related (PR) 10a Promoter and Confers Reduced Susceptibility to Pathogens." The New Phytologist, vol. 208, no. 3, 2015, pp. 846-59.
Choi C, Hwang SH, Fang IR, et al. Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens. New Phytol. 2015;208(3):846-59.
Choi, C., Hwang, S. H., Fang, I. R., Kwon, S. I., Park, S. R., Ahn, I., ... Hwang, D. J. (2015). Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens. The New Phytologist, 208(3), pp. 846-59. doi:10.1111/nph.13516.
Choi C, et al. Molecular Characterization of Oryza Sativa WRKY6, Which Binds to W-box-like Element 1 of the Oryza Sativa Pathogenesis-related (PR) 10a Promoter and Confers Reduced Susceptibility to Pathogens. New Phytol. 2015;208(3):846-59. PubMed PMID: 26083148.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of Oryza sativa WRKY6, which binds to W-box-like element 1 of the Oryza sativa pathogenesis-related (PR) 10a promoter and confers reduced susceptibility to pathogens. AU - Choi,Changhyun, AU - Hwang,Seon-Hee, AU - Fang,Il Ran, AU - Kwon,Soon Il, AU - Park,Sang Ryeol, AU - Ahn,Ilpyung, AU - Kim,Jung Bong, AU - Hwang,Duk-Ju, Y1 - 2015/06/17/ PY - 2015/03/27/received PY - 2015/05/11/accepted PY - 2015/6/18/entrez PY - 2015/6/18/pubmed PY - 2016/8/16/medline KW - OsWRKY6 KW - W-box-like element 1 (WLE1) KW - defense response KW - rice (Oryza sativa) KW - salicylic acid (SA) KW - ubiquitination SP - 846 EP - 59 JF - The New phytologist JO - New Phytol. VL - 208 IS - 3 N2 - WRKY proteins are transcription factors (TFs) that regulate the expression of defense-related genes. The salicylic acid (SA)-inducible Oryza sativa WRKY6 (OsWRKY6) was identified as a positive regulator of Oryza sativa pathogenesis-related 10a (OsPR10a) by transient expression assays. A physical interaction between OsWRKY6 and W-box-like element 1 (WLE1), which positively regulates OsPR10a/probenazole induced protein 1 expression, was verified in vitro. Several pathogenesis-related (PR) genes were constitutively activated, including OsPR10a, and transgenic rice (Oryza sativa) plants overexpressing (ox) OsWRKY6 exhibited enhanced disease resistance to pathogens. By contrast, PR gene induction was compromised in transgenic OsWRKY6-RNAi lines, suggesting that OsWRKY6 is a positive regulator of defense responses. OsWRKY6-ox lines displayed leaf lesions, and increased OsWRKY6 levels caused cell death. Salicylic acid (SA) concentrations were higher in OsWRKY6-ox lines than in wild-type (WT) plants, and transcript levels of Oryza sativa isochorismate synthase 1 (OsICS1), which encodes a major enzyme involved in SA biosynthesis, were higher in OsWRKY6-ox lines than in WT. OsWRKY6 directly bound to the OsICS1 promoter in vivo. This indicates that OsWRKY6 can directly regulate OsICS1 expression and thereby increase SA concentrations. OsWRKY6 autoregulates its own expression. OsWRKY6 protein degradation is possibly regulated by ubiquitination. Our results suggest that OsWRKY6 positively regulates defense responses through activation of OsICS1 expression and OsWRKY6 stabilization. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/26083148/Molecular_characterization_of_Oryza_sativa_WRKY6_which_binds_to_W_box_like_element_1_of_the_Oryza_sativa_pathogenesis_related__PR__10a_promoter_and_confers_reduced_susceptibility_to_pathogens_ L2 - https://doi.org/10.1111/nph.13516 DB - PRIME DP - Unbound Medicine ER -