Tags

Type your tag names separated by a space and hit enter

Metagenome changes in the mesophilic biogas-producing community during fermentation of the green alga Scenedesmus obliquus.
J Biotechnol. 2015 Dec 10; 215:52-61.JB

Abstract

A microalgal biomass offers a potential alternative to the maize silage commonly used in biogas technology. In this study, photoautotrophically grown Scenedesmus obliquus was used as biogas substrate. This microalga has a low C/N ratio of 8.5 relative to the optimum 20-30. A significant increase in the ammonium ion content was not observed. The methane content of the biogas generated from Sc. obliquus proved to be higher than that from maize silage, but the specific biogas yield was lower. Semi-continuous steady biogas production lasted for 2 months. Because of the thick cell wall of Sc. obliquus, the biomass-degrading microorganisms require additional time to digest its biomass. The methane concentration in the biogas was also high, in co-digestion (i.e., 52-56%) as in alga-fed anaerobic digestion (i.e., 55-62%). These results may be related to the relative predominance of the order Clostridiales in co-digestion and to the more balanced C/N ratio of the mixed algal-maize biomass. Predominance of the order Methanosarcinales was observed in the domain Archaea, which supported the diversity of metabolic pathways in the process.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Wirth R
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary. Electronic address: roland.w@freemail.hu.
Lakatos G
Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: lakatos.gergely@brc.mta.hu.
Böjti T
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary. Electronic address: tamas.bojti@gmail.com.
Maróti G
Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: maroti.gergely@brc.mta.hu.
Bagi Z
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary. Electronic address: bagiz@brc.hu.
Kis M
Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: kis.mihaly@brc.mta.hu.
Kovács A
Phytoplankton and Macrophyte Research Team, Balaton Limnological Institute, Klebersberg Kuno 3, H-8237 Tihany, Hungary. Electronic address: kovacs.attila@okologia.mta.hu.
Ács N
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary. Electronic address: acsn@brc.hu.
Rákhely G
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary; Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary. Electronic address: rakhely@brc.hu.
Kovács KL
Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary; Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Temesvári krt. 62, H-6726 Szeged, Hungary; Department of Oral Biology and Experimental Dental Research, University of Szeged, Tisza L. krt. 64, H-6720 Szeged, Hungary. Electronic address: kovacs.kornel@brc.mta.hu.

MeSH

Ammonium CompoundsArchaeaBiofuelsBiomassBioreactorsFermentationMetagenomeMethaneMethanosarcinalesMicroalgaeScenedesmusSilageZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26087313

Citation

Wirth, Roland, et al. "Metagenome Changes in the Mesophilic Biogas-producing Community During Fermentation of the Green Alga Scenedesmus Obliquus." Journal of Biotechnology, vol. 215, 2015, pp. 52-61.
Wirth R, Lakatos G, Böjti T, et al. Metagenome changes in the mesophilic biogas-producing community during fermentation of the green alga Scenedesmus obliquus. J Biotechnol. 2015;215:52-61.
Wirth, R., Lakatos, G., Böjti, T., Maróti, G., Bagi, Z., Kis, M., Kovács, A., Ács, N., Rákhely, G., & Kovács, K. L. (2015). Metagenome changes in the mesophilic biogas-producing community during fermentation of the green alga Scenedesmus obliquus. Journal of Biotechnology, 215, 52-61. https://doi.org/10.1016/j.jbiotec.2015.06.396
Wirth R, et al. Metagenome Changes in the Mesophilic Biogas-producing Community During Fermentation of the Green Alga Scenedesmus Obliquus. J Biotechnol. 2015 Dec 10;215:52-61. PubMed PMID: 26087313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metagenome changes in the mesophilic biogas-producing community during fermentation of the green alga Scenedesmus obliquus. AU - Wirth,Roland, AU - Lakatos,Gergely, AU - Böjti,Tamás, AU - Maróti,Gergely, AU - Bagi,Zoltán, AU - Kis,Mihály, AU - Kovács,Attila, AU - Ács,Norbert, AU - Rákhely,Gábor, AU - Kovács,Kornél L, Y1 - 2015/06/15/ PY - 2014/12/12/received PY - 2015/06/08/revised PY - 2015/06/12/accepted PY - 2015/6/19/entrez PY - 2015/6/19/pubmed PY - 2016/5/28/medline KW - Biogas KW - Metagenomics KW - Microalgae KW - Microbial community KW - Next-generation sequencing KW - Scenedesmus obliquus SP - 52 EP - 61 JF - Journal of biotechnology JO - J Biotechnol VL - 215 N2 - A microalgal biomass offers a potential alternative to the maize silage commonly used in biogas technology. In this study, photoautotrophically grown Scenedesmus obliquus was used as biogas substrate. This microalga has a low C/N ratio of 8.5 relative to the optimum 20-30. A significant increase in the ammonium ion content was not observed. The methane content of the biogas generated from Sc. obliquus proved to be higher than that from maize silage, but the specific biogas yield was lower. Semi-continuous steady biogas production lasted for 2 months. Because of the thick cell wall of Sc. obliquus, the biomass-degrading microorganisms require additional time to digest its biomass. The methane concentration in the biogas was also high, in co-digestion (i.e., 52-56%) as in alga-fed anaerobic digestion (i.e., 55-62%). These results may be related to the relative predominance of the order Clostridiales in co-digestion and to the more balanced C/N ratio of the mixed algal-maize biomass. Predominance of the order Methanosarcinales was observed in the domain Archaea, which supported the diversity of metabolic pathways in the process. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/26087313/Metagenome_changes_in_the_mesophilic_biogas_producing_community_during_fermentation_of_the_green_alga_Scenedesmus_obliquus_ DB - PRIME DP - Unbound Medicine ER -