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Inoculation of Nicotiana tabacum with recombinant potato virus X induces RNA interference in the solenopsis mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae).
Biotechnol Lett. 2015 Oct; 37(10):2083-90.BL

Abstract

OBJECTIVES

The chitin synthase 1 (CHS1) gene in Phenacoccus solenopsis (PsCHS1) was evaluated as a potential target of RNA interference (RNAi) by using Potato virus X (PVX) as a vector (recombinant PVX) for expressing RNAi triggering elements in Nicotiana tabacum L.

RESULTS

RT-PCR analysis confirmed the expression of PsCHS1 in N. tabacum inoculated with recombinant-PVX-PsCHS1 (treated). RT- and multiplex-PCR further showed a reduction in mRNA levels of the target gene in mealybugs feeding on treated plants. Mortality in parent adults and emerging nymphs (21 and 29%) exposed to the treated plants was significantly higher (P < 0.05) than those exposed to uninoculated (-ve control) or inoculated with non-recombinant PVX (PVX-control). The number of surviving adults and the combined number of adults and nymphs (47 and 60%) was significantly (P < 0.05) lower on the treated plants than the -ve (76%) or PVX (74%) control. The visual observations verified the physical deformities in mealybugs exposed to the treated plants.

CONCLUSION

chitin synthase 1 is a potential RNAi target in P. solenopsis and the recombinant PVX can be used as a tool to evaluate candidate RNAi triggering elements in plants.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Khan AM
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan. Department of Biotechnology, University of Sargodha, Sargodha, Pakistan.
Ashfaq M
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan. mashfaq@uoguelph.ca. Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada. mashfaq@uoguelph.ca.
Khan AA
College of Agriculture, Bahauddin Zakariya University, Bahadur Campus Layyah, Multan, Pakistan.
Rasool A
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Iqbal J
School of Life Sciences, Beijing Institute of Technology, Beijing, People's Republic of China.
Mansoor S
National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.

MeSH

AnimalsChitinasesHemipteraMultiplex Polymerase Chain ReactionNymphPlantsPotexvirusRNA InterferenceReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSurvival AnalysisTobacco

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26087945

Citation

Khan, Arif Muhammad, et al. "Inoculation of Nicotiana Tabacum With Recombinant Potato Virus X Induces RNA Interference in the Solenopsis Mealybug, Phenacoccus Solenopsis Tinsley (Hemiptera: Pseudococcidae)." Biotechnology Letters, vol. 37, no. 10, 2015, pp. 2083-90.
Khan AM, Ashfaq M, Khan AA, et al. Inoculation of Nicotiana tabacum with recombinant potato virus X induces RNA interference in the solenopsis mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). Biotechnol Lett. 2015;37(10):2083-90.
Khan, A. M., Ashfaq, M., Khan, A. A., Rasool, A., Iqbal, J., & Mansoor, S. (2015). Inoculation of Nicotiana tabacum with recombinant potato virus X induces RNA interference in the solenopsis mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). Biotechnology Letters, 37(10), 2083-90. https://doi.org/10.1007/s10529-015-1880-7
Khan AM, et al. Inoculation of Nicotiana Tabacum With Recombinant Potato Virus X Induces RNA Interference in the Solenopsis Mealybug, Phenacoccus Solenopsis Tinsley (Hemiptera: Pseudococcidae). Biotechnol Lett. 2015;37(10):2083-90. PubMed PMID: 26087945.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inoculation of Nicotiana tabacum with recombinant potato virus X induces RNA interference in the solenopsis mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). AU - Khan,Arif Muhammad, AU - Ashfaq,Muhammad, AU - Khan,Azhar Abbas, AU - Rasool,Akhtar, AU - Iqbal,Javed, AU - Mansoor,Shahid, Y1 - 2015/06/19/ PY - 2015/02/23/received PY - 2015/06/02/accepted PY - 2015/6/20/entrez PY - 2015/6/20/pubmed PY - 2016/6/9/medline KW - Mealy bug KW - Phenacoccus solenopsis KW - Potato virus X KW - RNA interference KW - Recombinant virus KW - Solenopsis mealybug SP - 2083 EP - 90 JF - Biotechnology letters JO - Biotechnol Lett VL - 37 IS - 10 N2 - OBJECTIVES: The chitin synthase 1 (CHS1) gene in Phenacoccus solenopsis (PsCHS1) was evaluated as a potential target of RNA interference (RNAi) by using Potato virus X (PVX) as a vector (recombinant PVX) for expressing RNAi triggering elements in Nicotiana tabacum L. RESULTS: RT-PCR analysis confirmed the expression of PsCHS1 in N. tabacum inoculated with recombinant-PVX-PsCHS1 (treated). RT- and multiplex-PCR further showed a reduction in mRNA levels of the target gene in mealybugs feeding on treated plants. Mortality in parent adults and emerging nymphs (21 and 29%) exposed to the treated plants was significantly higher (P < 0.05) than those exposed to uninoculated (-ve control) or inoculated with non-recombinant PVX (PVX-control). The number of surviving adults and the combined number of adults and nymphs (47 and 60%) was significantly (P < 0.05) lower on the treated plants than the -ve (76%) or PVX (74%) control. The visual observations verified the physical deformities in mealybugs exposed to the treated plants. CONCLUSION: chitin synthase 1 is a potential RNAi target in P. solenopsis and the recombinant PVX can be used as a tool to evaluate candidate RNAi triggering elements in plants. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/26087945/Inoculation_of_Nicotiana_tabacum_with_recombinant_potato_virus_X_induces_RNA_interference_in_the_solenopsis_mealybug_Phenacoccus_solenopsis_Tinsley__Hemiptera:_Pseudococcidae__ DB - PRIME DP - Unbound Medicine ER -