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Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing D-xylose fermentation by recombinant Saccharomyces cerevisiae.
Biotechnol Lett. 2015 Oct; 37(10):1973-82.BL

Abstract

OBJECTIVES

Since uptake of xylose limits its fermentation, we aimed to identify novel sugar transporters from Scheffersomyces stipitis that allow xylose uptake and fermentation by engineered Saccharomyces cerevisiae.

RESULTS

An hxt-null S. cerevisiae strain, lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) but having high xylose reductase, xylitol dehydrogenase and xylulokinase activities, was transformed with a genomic DNA library from S. stipitis. Four plasmids allowing growth on xylose contained three genes encoding sugar transporters: the previously characterized XUT1 permease, and two new genes (HXT2.6 and QUP2) not previously identified as xylose transporters. High cell density fermentations with the recombinant strains showed that the XUT1 gene allowed ethanol production from xylose or xylose plus glucose as carbon sources, while the HXT2.6 permease produced both ethanol and xylitol, and the strain expressing the QUP2 gene produced mainly xylitol during xylose consumption.

CONCLUSIONS

Cloning novel sugar transporters not previously identified in the S. stipitis genome using an hxt-null S. cerevisiae strain with a high xylose-utilizing pathway provides novel promising target genes for improved lignocellulosic ethanol production by yeasts.

Authors+Show Affiliations

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil.Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan.Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio De Janeiro, RJ, 21941-909, Brazil.Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, 88040-900, Brazil. boris.stambuk@ufsc.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26087949

Citation

de Sales, Belisa B., et al. "Cloning Novel Sugar Transporters From Scheffersomyces (Pichia) Stipitis Allowing D-xylose Fermentation By Recombinant Saccharomyces Cerevisiae." Biotechnology Letters, vol. 37, no. 10, 2015, pp. 1973-82.
de Sales BB, Scheid B, Gonçalves DL, et al. Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing D-xylose fermentation by recombinant Saccharomyces cerevisiae. Biotechnol Lett. 2015;37(10):1973-82.
de Sales, B. B., Scheid, B., Gonçalves, D. L., Knychala, M. M., Matsushika, A., Bon, E. P., & Stambuk, B. U. (2015). Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing D-xylose fermentation by recombinant Saccharomyces cerevisiae. Biotechnology Letters, 37(10), 1973-82. https://doi.org/10.1007/s10529-015-1893-2
de Sales BB, et al. Cloning Novel Sugar Transporters From Scheffersomyces (Pichia) Stipitis Allowing D-xylose Fermentation By Recombinant Saccharomyces Cerevisiae. Biotechnol Lett. 2015;37(10):1973-82. PubMed PMID: 26087949.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing D-xylose fermentation by recombinant Saccharomyces cerevisiae. AU - de Sales,Belisa B, AU - Scheid,Bruna, AU - Gonçalves,Davi L, AU - Knychala,Marilia M, AU - Matsushika,Akinori, AU - Bon,Elba P S, AU - Stambuk,Boris U, Y1 - 2015/06/19/ PY - 2015/05/07/received PY - 2015/06/11/accepted PY - 2015/6/20/entrez PY - 2015/6/20/pubmed PY - 2016/6/9/medline KW - Bioethanol KW - Fermentation KW - Saccharomyces cerevisiae KW - Scheffersomyces stipitis KW - Sugar transporter KW - Xylose SP - 1973 EP - 82 JF - Biotechnology letters JO - Biotechnol Lett VL - 37 IS - 10 N2 - OBJECTIVES: Since uptake of xylose limits its fermentation, we aimed to identify novel sugar transporters from Scheffersomyces stipitis that allow xylose uptake and fermentation by engineered Saccharomyces cerevisiae. RESULTS: An hxt-null S. cerevisiae strain, lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) but having high xylose reductase, xylitol dehydrogenase and xylulokinase activities, was transformed with a genomic DNA library from S. stipitis. Four plasmids allowing growth on xylose contained three genes encoding sugar transporters: the previously characterized XUT1 permease, and two new genes (HXT2.6 and QUP2) not previously identified as xylose transporters. High cell density fermentations with the recombinant strains showed that the XUT1 gene allowed ethanol production from xylose or xylose plus glucose as carbon sources, while the HXT2.6 permease produced both ethanol and xylitol, and the strain expressing the QUP2 gene produced mainly xylitol during xylose consumption. CONCLUSIONS: Cloning novel sugar transporters not previously identified in the S. stipitis genome using an hxt-null S. cerevisiae strain with a high xylose-utilizing pathway provides novel promising target genes for improved lignocellulosic ethanol production by yeasts. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/26087949/Cloning_novel_sugar_transporters_from_Scheffersomyces__Pichia__stipitis_allowing_D_xylose_fermentation_by_recombinant_Saccharomyces_cerevisiae_ L2 - https://doi.org/10.1007/s10529-015-1893-2 DB - PRIME DP - Unbound Medicine ER -