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Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length.
Theor Appl Genet. 2015 Sep; 128(9):1827-37.TA

Abstract

This study explored the genetic constitutions of wheat-Agropyron cristatum 6P chromosomal translocation and determined the effects of 6P intercalary chromosome segment on thousand-grain weight and spike length in wheat. Crop wild relatives provide rich genetic resources for wheat improvement. Introduction of alien genes from Agropyron cristatum into common wheat can broaden its genetic diversity. In this study, radiation-induced wheat-A. cristatum translocation line Pubing3035 derived from the offspring of wheat-A. cristatum 6P chromosomes addition line was identified and analyzed using genomic in situ hybridization (GISH), dual-color fluorescence in situ hybridization (FISH), and molecular markers. GISH analysis revealed that Pubing3035 was a Ti1AS-6PL-1AS·1AL intercalary translocation. The breakpoint was pinpointed to locate near the centromeric region on the short arm of wheat chromosome 1A based on a constructed F2 linkage map and it was flanked by markers SSR12 and SSR263. The genotypic data, combined with the phenotypes, indicated that A. cristatum 6P chromosomal segment played an important role in regulating the thousand-grain weight and spike length. On average, the thousand-grain weight and spike length in translocation individuals were approximately 2.5 g higher and 0.7 cm longer than those in non-translocation individuals in F2 and BC1F1 populations. The clusters of quantitative trait loci for thousand-grain weight, spike length, and spikelet density contributed by 6P chromosome segment were mapped between A. cristatum unique marker Agc7155 and wheat marker SSR263, which, respectively, explained phenotypic variance of 24.96, 12.38 and 17.20 % with an LOD of 10.63, 4.89 and 5.59. Overall, the translocation Pubing3035 had a positive effect on the yield of wheat, which laid the foundation for the localization of A. cristatum excellent genes and made itself a promising and valuable germplasm for wheat improvement.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26093609

Citation

Zhang, Jing, et al. "Introgression of Agropyron Cristatum 6P Chromosome Segment Into Common Wheat for Enhanced Thousand-grain Weight and Spike Length." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 128, no. 9, 2015, pp. 1827-37.
Zhang J, Zhang J, Liu W, et al. Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length. Theor Appl Genet. 2015;128(9):1827-37.
Zhang, J., Zhang, J., Liu, W., Han, H., Lu, Y., Yang, X., Li, X., & Li, L. (2015). Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 128(9), 1827-37. https://doi.org/10.1007/s00122-015-2550-9
Zhang J, et al. Introgression of Agropyron Cristatum 6P Chromosome Segment Into Common Wheat for Enhanced Thousand-grain Weight and Spike Length. Theor Appl Genet. 2015;128(9):1827-37. PubMed PMID: 26093609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Introgression of Agropyron cristatum 6P chromosome segment into common wheat for enhanced thousand-grain weight and spike length. AU - Zhang,Jing, AU - Zhang,Jinpeng, AU - Liu,Weihua, AU - Han,Haiming, AU - Lu,Yuqing, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Li,Lihui, Y1 - 2015/06/21/ PY - 2015/04/02/received PY - 2015/05/29/accepted PY - 2015/6/22/entrez PY - 2015/6/22/pubmed PY - 2015/12/22/medline SP - 1827 EP - 37 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 128 IS - 9 N2 - This study explored the genetic constitutions of wheat-Agropyron cristatum 6P chromosomal translocation and determined the effects of 6P intercalary chromosome segment on thousand-grain weight and spike length in wheat. Crop wild relatives provide rich genetic resources for wheat improvement. Introduction of alien genes from Agropyron cristatum into common wheat can broaden its genetic diversity. In this study, radiation-induced wheat-A. cristatum translocation line Pubing3035 derived from the offspring of wheat-A. cristatum 6P chromosomes addition line was identified and analyzed using genomic in situ hybridization (GISH), dual-color fluorescence in situ hybridization (FISH), and molecular markers. GISH analysis revealed that Pubing3035 was a Ti1AS-6PL-1AS·1AL intercalary translocation. The breakpoint was pinpointed to locate near the centromeric region on the short arm of wheat chromosome 1A based on a constructed F2 linkage map and it was flanked by markers SSR12 and SSR263. The genotypic data, combined with the phenotypes, indicated that A. cristatum 6P chromosomal segment played an important role in regulating the thousand-grain weight and spike length. On average, the thousand-grain weight and spike length in translocation individuals were approximately 2.5 g higher and 0.7 cm longer than those in non-translocation individuals in F2 and BC1F1 populations. The clusters of quantitative trait loci for thousand-grain weight, spike length, and spikelet density contributed by 6P chromosome segment were mapped between A. cristatum unique marker Agc7155 and wheat marker SSR263, which, respectively, explained phenotypic variance of 24.96, 12.38 and 17.20 % with an LOD of 10.63, 4.89 and 5.59. Overall, the translocation Pubing3035 had a positive effect on the yield of wheat, which laid the foundation for the localization of A. cristatum excellent genes and made itself a promising and valuable germplasm for wheat improvement. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/26093609/Introgression_of_Agropyron_cristatum_6P_chromosome_segment_into_common_wheat_for_enhanced_thousand_grain_weight_and_spike_length_ DB - PRIME DP - Unbound Medicine ER -