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Neuregulin-1β Regulates the migration of Different Neurochemical Phenotypic Neurons from Organotypically Cultured Dorsal Root Ganglion Explants.
Cell Mol Neurobiol. 2016 Jan; 36(1):69-81.CM

Abstract

Neuregulin-1β (NRG-1β) has multiple roles in the development and function in the nervous system and exhibits potent neuroprotective properties. In the present study, organotypically cultured dorsal root ganglion (DRG) explants were used to evaluate the effects of NRG-1β on migration of two major phenotypic classes of DRG neurons. The signaling pathways involved in these effects were also determined. Organotypically cultured DRG explants were exposed to NRG-1β (20 nmol/L), the phosphatidylinositol 3-kinase inhibitor LY294002 (10 μmol/L) plus NRG-1β (20 nmol/L), the extracellular signal-regulated protein kinase (ERK1/2) inhibitor PD98059 (10 μmol/L) plus NRG-1β (20 nmol/L), and LY294002 (10 μmol/L) plus PD98059 (10 μmol/L) plus NRG-1β (20 nmol/L), respectively, for 3 days. The DRG explants were continuously exposed to culture media as a control. After that, all above cultures were processed for detecting the mRNA levels of calcitonin gene-related peptide (CGRP) and neurofilament-200 (NF-200) by real-time PCR analysis. CGRP and NF-200 expression in situ was determined by fluorescent labeling technique. The results showed that NRG-1β elevated the mRNA and protein levels of CGRP and NF-200. NRG-1β also increased the number and the percentage of CGRP-immunoreactive (IR) migrating neurons and NF-200-IR migrating neurons. Inhibitors (LY294002, PD98059) either alone or in combination blocked the effects of NRG-1β. The contribution of NRG-1β on modulating distinct neurochemical phenotypic plasticity of DRG neurons suggested that NRG-1β signaling system might play an important role on the biological effects of primary sensory neurons.

Authors+Show Affiliations

Department of Cardiosurgery, Shandong University Qilu Hospital, 107 West Wenhua Xi Road, Jinan, 250012, Shandong Province, China. yunfengli630@live.com.Department of Histology and Embryology, Binzhou Medical College, Binzhou, 256603, China. liuguixiang57@163.com.Department of Orthopaedics, Shandong University Qilu Hospital, Jinan, 250012, China. lihao462929@163.com.Department of Cardiosurgery, Shandong University Qilu Hospital, 107 West Wenhua Xi Road, Jinan, 250012, Shandong Province, China. yanwenbi126@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26093851

Citation

Li, Yunfeng, et al. "Neuregulin-1β Regulates the Migration of Different Neurochemical Phenotypic Neurons From Organotypically Cultured Dorsal Root Ganglion Explants." Cellular and Molecular Neurobiology, vol. 36, no. 1, 2016, pp. 69-81.
Li Y, Liu G, Li H, et al. Neuregulin-1β Regulates the migration of Different Neurochemical Phenotypic Neurons from Organotypically Cultured Dorsal Root Ganglion Explants. Cell Mol Neurobiol. 2016;36(1):69-81.
Li, Y., Liu, G., Li, H., & Bi, Y. (2016). Neuregulin-1β Regulates the migration of Different Neurochemical Phenotypic Neurons from Organotypically Cultured Dorsal Root Ganglion Explants. Cellular and Molecular Neurobiology, 36(1), 69-81. https://doi.org/10.1007/s10571-015-0221-7
Li Y, et al. Neuregulin-1β Regulates the Migration of Different Neurochemical Phenotypic Neurons From Organotypically Cultured Dorsal Root Ganglion Explants. Cell Mol Neurobiol. 2016;36(1):69-81. PubMed PMID: 26093851.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuregulin-1β Regulates the migration of Different Neurochemical Phenotypic Neurons from Organotypically Cultured Dorsal Root Ganglion Explants. AU - Li,Yunfeng, AU - Liu,Guixiang, AU - Li,Hao, AU - Bi,Yanwen, Y1 - 2015/06/21/ PY - 2015/02/12/received PY - 2015/06/02/accepted PY - 2015/6/22/entrez PY - 2015/6/22/pubmed PY - 2016/10/8/medline KW - Calcitonin gene-related peptide KW - Dorsal root ganglion KW - Neuregulin-1β KW - Neurofilament 200 KW - Neuron KW - Phenotype SP - 69 EP - 81 JF - Cellular and molecular neurobiology JO - Cell. Mol. Neurobiol. VL - 36 IS - 1 N2 - Neuregulin-1β (NRG-1β) has multiple roles in the development and function in the nervous system and exhibits potent neuroprotective properties. In the present study, organotypically cultured dorsal root ganglion (DRG) explants were used to evaluate the effects of NRG-1β on migration of two major phenotypic classes of DRG neurons. The signaling pathways involved in these effects were also determined. Organotypically cultured DRG explants were exposed to NRG-1β (20 nmol/L), the phosphatidylinositol 3-kinase inhibitor LY294002 (10 μmol/L) plus NRG-1β (20 nmol/L), the extracellular signal-regulated protein kinase (ERK1/2) inhibitor PD98059 (10 μmol/L) plus NRG-1β (20 nmol/L), and LY294002 (10 μmol/L) plus PD98059 (10 μmol/L) plus NRG-1β (20 nmol/L), respectively, for 3 days. The DRG explants were continuously exposed to culture media as a control. After that, all above cultures were processed for detecting the mRNA levels of calcitonin gene-related peptide (CGRP) and neurofilament-200 (NF-200) by real-time PCR analysis. CGRP and NF-200 expression in situ was determined by fluorescent labeling technique. The results showed that NRG-1β elevated the mRNA and protein levels of CGRP and NF-200. NRG-1β also increased the number and the percentage of CGRP-immunoreactive (IR) migrating neurons and NF-200-IR migrating neurons. Inhibitors (LY294002, PD98059) either alone or in combination blocked the effects of NRG-1β. The contribution of NRG-1β on modulating distinct neurochemical phenotypic plasticity of DRG neurons suggested that NRG-1β signaling system might play an important role on the biological effects of primary sensory neurons. SN - 1573-6830 UR - https://www.unboundmedicine.com/medline/citation/26093851/Neuregulin_1β_Regulates_the_migration_of_Different_Neurochemical_Phenotypic_Neurons_from_Organotypically_Cultured_Dorsal_Root_Ganglion_Explants_ L2 - https://doi.org/10.1007/s10571-015-0221-7 DB - PRIME DP - Unbound Medicine ER -