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Phytophthora capsici homologue of the cell cycle regulator SDA1 is required for sporangial morphology, mycelial growth and plant infection.
Mol Plant Pathol. 2016 Apr; 17(3):369-87.MP

Abstract

SDA1 encodes a highly conserved protein that is widely distributed in eukaryotic organisms. SDA1 is essential for cell cycle progression and organization of the actin cytoskeleton in yeasts and humans. In this study, we identified a Phytophthora capsici orthologue of yeast SDA1, named PcSDA1. In P. capsici, PcSDA1 is strongly expressed in three asexual developmental states (mycelium, sporangia and germinating cysts), as well as late in infection. Silencing or overexpression of PcSDA1 in P. capsici transformants affected the growth of hyphae and sporangiophores, sporangial development, cyst germination and zoospore release. Phalloidin staining confirmed that PcSDA1 is required for organization of the actin cytoskeleton. Moreover, 4',6-diamidino-2-phenylindole (DAPI) staining and PcSDA1-green fluorescent protein (GFP) fusions revealed that PcSDA1 is involved in the regulation of nuclear distribution in hyphae and sporangia. Both silenced and overexpression transformants showed severely diminished virulence. Thus, our results suggest that PcSDA1 plays a similar role in the regulation of the actin cytoskeleton and nuclear division in this filamentous organism as in non-filamentous yeasts and human cells.

Authors+Show Affiliations

Department of Plant Pathology, Shandong Agricultural University, 61, Daizong Street, Tai'an, Shandong, 271018, China.Department of Plant Pathology, Shandong Agricultural University, 61, Daizong Street, Tai'an, Shandong, 271018, China.Department of Plant Pathology, Shandong Agricultural University, 61, Daizong Street, Tai'an, Shandong, 271018, China.Department of Plant Pathology, Shandong Agricultural University, 61, Daizong Street, Tai'an, Shandong, 271018, China.Department of Plant Pathology, Shandong Agricultural University, 61, Daizong Street, Tai'an, Shandong, 271018, China.Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR, 97331, USA.Department of Plant Pathology, Shandong Agricultural University, 61, Daizong Street, Tai'an, Shandong, 271018, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26095317

Citation

Zhu, Chunyuan, et al. "Phytophthora Capsici Homologue of the Cell Cycle Regulator SDA1 Is Required for Sporangial Morphology, Mycelial Growth and Plant Infection." Molecular Plant Pathology, vol. 17, no. 3, 2016, pp. 369-87.
Zhu C, Yang X, Lv R, et al. Phytophthora capsici homologue of the cell cycle regulator SDA1 is required for sporangial morphology, mycelial growth and plant infection. Mol Plant Pathol. 2016;17(3):369-87.
Zhu, C., Yang, X., Lv, R., Li, Z., Ding, X., Tyler, B. M., & Zhang, X. (2016). Phytophthora capsici homologue of the cell cycle regulator SDA1 is required for sporangial morphology, mycelial growth and plant infection. Molecular Plant Pathology, 17(3), 369-87. https://doi.org/10.1111/mpp.12285
Zhu C, et al. Phytophthora Capsici Homologue of the Cell Cycle Regulator SDA1 Is Required for Sporangial Morphology, Mycelial Growth and Plant Infection. Mol Plant Pathol. 2016;17(3):369-87. PubMed PMID: 26095317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytophthora capsici homologue of the cell cycle regulator SDA1 is required for sporangial morphology, mycelial growth and plant infection. AU - Zhu,Chunyuan, AU - Yang,Xiaoyan, AU - Lv,Rongfei, AU - Li,Zhuang, AU - Ding,Xiaomeng, AU - Tyler,Brett M, AU - Zhang,Xiuguo, Y1 - 2015/08/27/ PY - 2015/6/23/entrez PY - 2015/6/23/pubmed PY - 2016/12/15/medline KW - PcSDA1 KW - Phytophthora capsici KW - actin cytoskeleton KW - asexual development KW - nuclear localization KW - virulence SP - 369 EP - 87 JF - Molecular plant pathology JO - Mol. Plant Pathol. VL - 17 IS - 3 N2 - SDA1 encodes a highly conserved protein that is widely distributed in eukaryotic organisms. SDA1 is essential for cell cycle progression and organization of the actin cytoskeleton in yeasts and humans. In this study, we identified a Phytophthora capsici orthologue of yeast SDA1, named PcSDA1. In P. capsici, PcSDA1 is strongly expressed in three asexual developmental states (mycelium, sporangia and germinating cysts), as well as late in infection. Silencing or overexpression of PcSDA1 in P. capsici transformants affected the growth of hyphae and sporangiophores, sporangial development, cyst germination and zoospore release. Phalloidin staining confirmed that PcSDA1 is required for organization of the actin cytoskeleton. Moreover, 4',6-diamidino-2-phenylindole (DAPI) staining and PcSDA1-green fluorescent protein (GFP) fusions revealed that PcSDA1 is involved in the regulation of nuclear distribution in hyphae and sporangia. Both silenced and overexpression transformants showed severely diminished virulence. Thus, our results suggest that PcSDA1 plays a similar role in the regulation of the actin cytoskeleton and nuclear division in this filamentous organism as in non-filamentous yeasts and human cells. SN - 1364-3703 UR - https://www.unboundmedicine.com/medline/citation/26095317/Phytophthora_capsici_homologue_of_the_cell_cycle_regulator_SDA1_is_required_for_sporangial_morphology_mycelial_growth_and_plant_infection_ L2 - https://doi.org/10.1111/mpp.12285 DB - PRIME DP - Unbound Medicine ER -