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In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against clinical isolates of the influenza virus circulating in the Japanese 2013-2014 season.

Abstract

To assess the extent of viral resistance, we measured the 50% inhibitory concentration (IC50) of neuraminidase inhibitors (NAIs) for the influenza virus isolates in the 2013-2014 influenza season and compared the results to those of the 2010-2011 to 2012-2013 influenza seasons. Viral isolation was done with specimens obtained prior to treatment, and the type and subtype of influenza was determined by RT-PCR using type- and subtype-specific primers. The IC50 was determined by a neuraminidase inhibition assay using a fluorescent substrate. A total of 327 influenza viruses were isolated: 172 influenza A(H1N1)pdm09 (52.6%), 49 A(H3N2) (15.0%), and 106 B (32.4%). Numbers of Victoria and Yamagata lineage isolates were 36 and 70, respectively. Two A(H1N1)pdm09 isolates showed a high IC50 for oseltamivir (130 and 150 nM) exceeding by 100 times the geometric mean of the IC50 of oseltamivir for A(H1N1)pdm09 isolates (0.76 nM). No isolate showed a very high IC50 for A(H3N2) or B. The IC50 of the NAIs except for oseltamivir for A(H1N1) pdm09 were significantly higher than those of the 2010-2011 season (P < 0.05). The IC50 of all four NAIs for A(H3N2) were significantly lower than those of the 2012-2013 season (P < 0.001). The IC50 of the NAIs for B except for oseltamivir were significantly lower than those of the 2012-2013 season (P < 0.001). Although there are some isolates that show highly reduced sensitivity to oseltamivir among A(H1N1)pdm09 isolates, the currently epidemic influenza A(H1N1)pdm09, A(H3N2) and B viruses are susceptible to all four NAIs with no trend toward decreased sensitivity.

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  • Authors+Show Affiliations

    ,

    Japan Physicians Association, Tokyo, Japan. Electronic address: ikematsu@gray.plala.or.jp.

    ,

    Japan Physicians Association, Tokyo, Japan.

    ,

    Japan Physicians Association, Tokyo, Japan.

    Japan Physicians Association, Tokyo, Japan.

    Source

    MeSH

    Adolescent
    Adult
    Aged
    Aged, 80 and over
    Child
    Child, Preschool
    Cyclopentanes
    Enzyme Inhibitors
    Female
    Guanidines
    Humans
    Infant
    Infant, Newborn
    Influenza A Virus, H1N1 Subtype
    Influenza A Virus, H3N2 Subtype
    Influenza B virus
    Influenza, Human
    Inhibitory Concentration 50
    Japan
    Male
    Middle Aged
    Neuraminidase
    Oseltamivir
    Time Factors
    Young Adult
    Zanamivir

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    26096495

    Citation

    Ikematsu, Hideyuki, et al. "In Vitro Neuraminidase Inhibitory Activity of Four Neuraminidase Inhibitors Against Clinical Isolates of the Influenza Virus Circulating in the Japanese 2013-2014 Season." Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, vol. 21, no. 9, 2015, pp. 634-8.
    Ikematsu H, Kawai N, Iwaki N, et al. In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against clinical isolates of the influenza virus circulating in the Japanese 2013-2014 season. J Infect Chemother. 2015;21(9):634-8.
    Ikematsu, H., Kawai, N., Iwaki, N., & Kashiwagi, S. (2015). In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against clinical isolates of the influenza virus circulating in the Japanese 2013-2014 season. Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, 21(9), pp. 634-8. doi:10.1016/j.jiac.2015.05.004.
    Ikematsu H, et al. In Vitro Neuraminidase Inhibitory Activity of Four Neuraminidase Inhibitors Against Clinical Isolates of the Influenza Virus Circulating in the Japanese 2013-2014 Season. J Infect Chemother. 2015;21(9):634-8. PubMed PMID: 26096495.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro neuraminidase inhibitory activity of four neuraminidase inhibitors against clinical isolates of the influenza virus circulating in the Japanese 2013-2014 season. AU - Ikematsu,Hideyuki, AU - Kawai,Naoki, AU - Iwaki,Norio, AU - Kashiwagi,Seizabro, Y1 - 2015/05/30/ PY - 2015/03/19/received PY - 2015/05/01/revised PY - 2015/05/21/accepted PY - 2015/6/23/entrez PY - 2015/6/23/pubmed PY - 2016/5/21/medline KW - 50% Inhibitory concentration KW - Influenza KW - Laninamivir KW - Oseltamivir KW - Peramivir KW - Zanamivir SP - 634 EP - 8 JF - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JO - J. Infect. Chemother. VL - 21 IS - 9 N2 - To assess the extent of viral resistance, we measured the 50% inhibitory concentration (IC50) of neuraminidase inhibitors (NAIs) for the influenza virus isolates in the 2013-2014 influenza season and compared the results to those of the 2010-2011 to 2012-2013 influenza seasons. Viral isolation was done with specimens obtained prior to treatment, and the type and subtype of influenza was determined by RT-PCR using type- and subtype-specific primers. The IC50 was determined by a neuraminidase inhibition assay using a fluorescent substrate. A total of 327 influenza viruses were isolated: 172 influenza A(H1N1)pdm09 (52.6%), 49 A(H3N2) (15.0%), and 106 B (32.4%). Numbers of Victoria and Yamagata lineage isolates were 36 and 70, respectively. Two A(H1N1)pdm09 isolates showed a high IC50 for oseltamivir (130 and 150 nM) exceeding by 100 times the geometric mean of the IC50 of oseltamivir for A(H1N1)pdm09 isolates (0.76 nM). No isolate showed a very high IC50 for A(H3N2) or B. The IC50 of the NAIs except for oseltamivir for A(H1N1) pdm09 were significantly higher than those of the 2010-2011 season (P < 0.05). The IC50 of all four NAIs for A(H3N2) were significantly lower than those of the 2012-2013 season (P < 0.001). The IC50 of the NAIs for B except for oseltamivir were significantly lower than those of the 2012-2013 season (P < 0.001). Although there are some isolates that show highly reduced sensitivity to oseltamivir among A(H1N1)pdm09 isolates, the currently epidemic influenza A(H1N1)pdm09, A(H3N2) and B viruses are susceptible to all four NAIs with no trend toward decreased sensitivity. SN - 1437-7780 UR - https://www.unboundmedicine.com/medline/citation/26096495/In_vitro_neuraminidase_inhibitory_activity_of_four_neuraminidase_inhibitors_against_clinical_isolates_of_the_influenza_virus_circulating_in_the_Japanese_2013_2014_season_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1341-321X(15)00124-5 DB - PRIME DP - Unbound Medicine ER -