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Diagnostic SNPs reveal widespread introgressive hybridization between introduced bighead and silver carp in the Mississippi River Basin.
Mol Ecol. 2015 Aug; 24(15):3931-43.ME

Abstract

Hybridization among conspecifics in native and introduced habitats has important implications for biological invasions in new ecosystems. Bighead (Hypophthalmichthys nobilis) and silver carp (H. molitrix) are genetically isolated and occur in sympatry within their native range. Following their introduction to North America, however, introgressant hybrids have been reported throughout their expanded range within the Mississippi River Basin (MRB). The extent of introgression, both spatially and generationally, is largely unknown. Therefore, we examined mixed-species populations from across the MRB to characterize the extent of interspecific gene flow. We assayed 2798 individuals from nine locations with a suite of species-diagnostic SNPs (57 nuclear and one mitochondrial). Forty-four per cent (n = 1244) of individuals displayed hybrid genotypes. Moreover, the composition of hybrid genotypes varied among locations and represented complex hybrid swarms with multiple generations of gene flow. Introgressive hybrids were identified from all locations, were bidirectional and followed a bimodal distribution consisting primarily of parental or parental-like genotypes and phenotypes. All described hybrid categories were present among individuals from 1999 to 2008, with parents and later-generation backcrosses representing the largest proportion of individuals among years. Our mitochondrial SNP (COII), tested on a subset of 730 individuals, revealed a silver carp maternal bias in 13 of 21 (62%) F1 hybrids, in all silver carp backcrosses, and maintained throughout many of the bighead carp backcrosses. The application of this suite of diagnostic markers and the spatial coverage permits a deeper examination of the complexity in hybrid swarms between two invasive, introduced species.

Authors+Show Affiliations

Department of Natural Resources and Environmental Sciences, University of Illinois, 1102 South Goodwin Ave., Urbana, IL, 61801, USA. Department of Biological Sciences, Western Illinois University, 1 University Circle, Macomb, IL, 61455, USA.Illinois Department of Natural Resources, One Natural Resources Way, Springfield, IL, 62702, USA.Core Genomics Facility, University of Illinois at Chicago, 835 S. Wolcott Avenue, Chicago, IL, 60612, USA.Illinois Department of Natural Resources, 700 S. 10th St., Havana, IL, 62644, USA.Illinois Natural History Survey, 1816 S. Oak Street, Champaign, IL, 61820, USA.Escanaba Lake Research Station, Wisconsin Department of Natural Resources, 10810 County Hwy N, Boulder Junction, WI, 54512, USA.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

26096550

Citation

Lamer, James T., et al. "Diagnostic SNPs Reveal Widespread Introgressive Hybridization Between Introduced Bighead and Silver Carp in the Mississippi River Basin." Molecular Ecology, vol. 24, no. 15, 2015, pp. 3931-43.
Lamer JT, Ruebush BC, Arbieva ZH, et al. Diagnostic SNPs reveal widespread introgressive hybridization between introduced bighead and silver carp in the Mississippi River Basin. Mol Ecol. 2015;24(15):3931-43.
Lamer, J. T., Ruebush, B. C., Arbieva, Z. H., McClelland, M. A., Epifanio, J. M., & Sass, G. G. (2015). Diagnostic SNPs reveal widespread introgressive hybridization between introduced bighead and silver carp in the Mississippi River Basin. Molecular Ecology, 24(15), 3931-43. https://doi.org/10.1111/mec.13285
Lamer JT, et al. Diagnostic SNPs Reveal Widespread Introgressive Hybridization Between Introduced Bighead and Silver Carp in the Mississippi River Basin. Mol Ecol. 2015;24(15):3931-43. PubMed PMID: 26096550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic SNPs reveal widespread introgressive hybridization between introduced bighead and silver carp in the Mississippi River Basin. AU - Lamer,James T, AU - Ruebush,Blake C, AU - Arbieva,Zarema H, AU - McClelland,Michael A, AU - Epifanio,John M, AU - Sass,Greg G, Y1 - 2015/07/17/ PY - 2015/03/14/received PY - 2015/05/31/revised PY - 2015/06/18/accepted PY - 2015/6/23/entrez PY - 2015/6/23/pubmed PY - 2015/10/6/medline KW - SNP KW - bighead carp KW - hybridization KW - introgression KW - invasive species KW - silver carp SP - 3931 EP - 43 JF - Molecular ecology JO - Mol. Ecol. VL - 24 IS - 15 N2 - Hybridization among conspecifics in native and introduced habitats has important implications for biological invasions in new ecosystems. Bighead (Hypophthalmichthys nobilis) and silver carp (H. molitrix) are genetically isolated and occur in sympatry within their native range. Following their introduction to North America, however, introgressant hybrids have been reported throughout their expanded range within the Mississippi River Basin (MRB). The extent of introgression, both spatially and generationally, is largely unknown. Therefore, we examined mixed-species populations from across the MRB to characterize the extent of interspecific gene flow. We assayed 2798 individuals from nine locations with a suite of species-diagnostic SNPs (57 nuclear and one mitochondrial). Forty-four per cent (n = 1244) of individuals displayed hybrid genotypes. Moreover, the composition of hybrid genotypes varied among locations and represented complex hybrid swarms with multiple generations of gene flow. Introgressive hybrids were identified from all locations, were bidirectional and followed a bimodal distribution consisting primarily of parental or parental-like genotypes and phenotypes. All described hybrid categories were present among individuals from 1999 to 2008, with parents and later-generation backcrosses representing the largest proportion of individuals among years. Our mitochondrial SNP (COII), tested on a subset of 730 individuals, revealed a silver carp maternal bias in 13 of 21 (62%) F1 hybrids, in all silver carp backcrosses, and maintained throughout many of the bighead carp backcrosses. The application of this suite of diagnostic markers and the spatial coverage permits a deeper examination of the complexity in hybrid swarms between two invasive, introduced species. SN - 1365-294X UR - https://www.unboundmedicine.com/medline/citation/26096550/Diagnostic_SNPs_reveal_widespread_introgressive_hybridization_between_introduced_bighead_and_silver_carp_in_the_Mississippi_River_Basin_ L2 - https://doi.org/10.1111/mec.13285 DB - PRIME DP - Unbound Medicine ER -