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Genotypes of ITS region of rRNA in Microcystis (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level.
J Basic Microbiol. 2015 Oct; 55(10):1203-11.JB

Abstract

Previous studies on spatiotemporal changes of Microcystis genotypes have shown that the existence and succession of dominant genotypes always occur in eutrophicated freshwater bodies. However, few studies have focused on the correlation between genotype composition and eutrophication level. In the present study, clone libraries of the internal transcribed spacer (ITS) of rrn operon were sequenced from Microcystis populations in Erhai Lake, a subtropical plateau lake in the preliminary eutrophication stage. The genotype composition of the Microcystis populations was highly variable at spatiotemporal scales, and 473 ITS genotypes were identified from the 800 ITS sequences obtained. However, no significantly dominant ITS genotypes existed in the lake. Comparison of Erhai Lake with four major lakes in China, namely, Taihu, Chaohu, Gucheng, and Shijiu Lakes, showed that the Microcystis ITS genotypes and genetic diversity were negatively correlated with eutrophication level. Extensive comparison of the Microcystis ITS genotypes from waters worldwide revealed that 440 ITS genotypes were unique to Erhai Lake, and no obvious phylogenetic correlations can be detected among the dominant genotypes from different water bodies. The high genetic diversity of the Microcystis populations in Erhai Lake may have resulted from the effect of the early stage of eutrophication.

Authors+Show Affiliations

Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P.R. China. Graduate University of Chinese Academy of Sciences, Beijing, P.R. China.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P.R. China. Shenzhen Key Laboratory for Marine Bio-resource and Eco-environment, College of Life Sciences, Shenzhen University, Shenzhen, P.R. China.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P.R. China.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, P.R. China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26098704

Citation

Song, Gaofei, et al. "Genotypes of ITS Region of rRNA in Microcystis (Cyanobacteria) Populations in Erhai Lake (China) and Their Correlation With Eutrophication Level." Journal of Basic Microbiology, vol. 55, no. 10, 2015, pp. 1203-11.
Song G, Jiang Y, Yu G, et al. Genotypes of ITS region of rRNA in Microcystis (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level. J Basic Microbiol. 2015;55(10):1203-11.
Song, G., Jiang, Y., Yu, G., & Li, R. (2015). Genotypes of ITS region of rRNA in Microcystis (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level. Journal of Basic Microbiology, 55(10), 1203-11. https://doi.org/10.1002/jobm.201500080
Song G, et al. Genotypes of ITS Region of rRNA in Microcystis (Cyanobacteria) Populations in Erhai Lake (China) and Their Correlation With Eutrophication Level. J Basic Microbiol. 2015;55(10):1203-11. PubMed PMID: 26098704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotypes of ITS region of rRNA in Microcystis (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level. AU - Song,Gaofei, AU - Jiang,Yongguang, AU - Yu,Gongliang, AU - Li,Renhui, Y1 - 2015/06/22/ PY - 2015/02/03/received PY - 2015/04/20/accepted PY - 2015/6/23/entrez PY - 2015/6/23/pubmed PY - 2016/6/15/medline KW - Diversity KW - Eutrophication level KW - ITS genotype KW - Microcystis bloom SP - 1203 EP - 11 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 55 IS - 10 N2 - Previous studies on spatiotemporal changes of Microcystis genotypes have shown that the existence and succession of dominant genotypes always occur in eutrophicated freshwater bodies. However, few studies have focused on the correlation between genotype composition and eutrophication level. In the present study, clone libraries of the internal transcribed spacer (ITS) of rrn operon were sequenced from Microcystis populations in Erhai Lake, a subtropical plateau lake in the preliminary eutrophication stage. The genotype composition of the Microcystis populations was highly variable at spatiotemporal scales, and 473 ITS genotypes were identified from the 800 ITS sequences obtained. However, no significantly dominant ITS genotypes existed in the lake. Comparison of Erhai Lake with four major lakes in China, namely, Taihu, Chaohu, Gucheng, and Shijiu Lakes, showed that the Microcystis ITS genotypes and genetic diversity were negatively correlated with eutrophication level. Extensive comparison of the Microcystis ITS genotypes from waters worldwide revealed that 440 ITS genotypes were unique to Erhai Lake, and no obvious phylogenetic correlations can be detected among the dominant genotypes from different water bodies. The high genetic diversity of the Microcystis populations in Erhai Lake may have resulted from the effect of the early stage of eutrophication. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/26098704/Genotypes_of_ITS_region_of_rRNA_in_Microcystis__Cyanobacteria__populations_in_Erhai_Lake__China__and_their_correlation_with_eutrophication_level_ L2 - https://doi.org/10.1002/jobm.201500080 DB - PRIME DP - Unbound Medicine ER -