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Does the gastroprotective action of a medicinal plant ensure healing effects? An integrative study of the biological effects of Serjania marginata Casar. (Sapindaceae) in rats.
J Ethnopharmacol. 2015 Aug 22; 172:312-24.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Serjania marginata (Sapindaceae), a medicinal plant commonly found in the Brazilian Cerrado, Paraguay, Bolivia and Argentina, is also known as "cipó-uva" or "cipó-timbó". Ethnopharmacological studies indicate that the leaves from this medicinal plant are used in folk medicine to treat gastric pain. The overall objective of this study was to evaluate the gastroprotective and healing effect of the hydroalcoholic extract obtained from S. marginata (HESM) leaves using rodent experimental models. As part of the integrative study of this medicinal plant, we also evaluated the acute toxicity, antimicrobial, antidiarrheal, (anti)mutagenic, and hemodynamic effects.

MATERIAL AND METHODS

We performed a pharmacological study to test the acute toxicity and antimutagenic effect (Ames assay) of the HESM. The HESM was tested against different necrosis-promoting agents and experimental manipulations, such as absolute ethanol, cysteamine, pyloric ligature, and ischemia-reperfusion (I/R) injury. The gastroprotective effect of the HESM was assessed by analyzing the gastric juice (volume, pH, total acidity) and the mucus in the gastric mucosa from rats. We assessed the levels of NO, sulfhydryl compounds, PGE2, vanilloid receptor, glutathione (GSH), and malondialdehyde (MDA), as well as the myeloperoxidase (MPO) activity. The gastric healing effects of the HESM were evaluated during 7 or 14 days of treatment. The intestinal motility, antidiarrheal action, and antibacterial effects (microdilution methods) of the HESM were also evaluated.

RESULTS

The phytochemical analysis of the HESM revealed the presence of saponins, flavonoid glycosides, and tannins. The extract exhibited no sign of acute toxicity or mutagenic effect in vitro. In contrast, this extract exhibited a protective effect against the mutagenic action of direct- and indirect-acting mutagens. Only the oral administration of HESM (250mg/kg) significantly decreased the severity of gastric damage induced by ethanol (60.13%) and I/R (58.31%). The HESM exerts its gastroprotective effects by decreasing the MPO and MDA activities in the gastric tissue and by increasing the amount of adherent mucus covering the gastric mucosa. In vitro, the extract also displayed evident antimicrobial effects against Helicobacter pylori. However, the preventive effect of the HESM was not accompanied by an ulcer-healing effect. The treatment with HESM (14 days) significantly increased gastric lesions in 99% of the tested animals compared with the control group. This result represents a highly relevant piece of evidence that should resonate as an alert against the chronic use of this medicinal plant as an antiulcer in folk medicine.

CONCLUSIONS

Despite the anti-H. pylori and gastroprotective actions of S. marginata in experimental models, the gastric injuries aggravation induced after chronic treatment with the HESM argues against the use of this plant species in folk medicine.

Authors+Show Affiliations

Univ. Estadual Paulista-UNESP, Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil.Universidade São Francisco, Faculdade de Ciências Médicas, Unidade Integrada de Farmacologia e Gastroenterologia, CEP 12916-900, Bragança Paulista, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Faculdade de Ciências Farmacêuticas, CEP 14800-902, Araraquara, SP, Brazil.Univ. Estadual Paulista-UNESP, Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP, Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil.Univ. Estadual Paulista-UNESP, Campus Experimental do Litoral Paulista, CEP 11330-900, São Vicente, SP, Brazil.Univ. Estadual Paulista-UNESP, Departamento de Fisiologia, Instituto de Biociências, CEP 18618-970, Botucatu, SP, Brazil. Electronic address: hiruma@ibb.unesp.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26099637

Citation

Périco, Larissa Lucena, et al. "Does the Gastroprotective Action of a Medicinal Plant Ensure Healing Effects? an Integrative Study of the Biological Effects of Serjania Marginata Casar. (Sapindaceae) in Rats." Journal of Ethnopharmacology, vol. 172, 2015, pp. 312-24.
Périco LL, Heredia-Vieira SC, Beserra FP, et al. Does the gastroprotective action of a medicinal plant ensure healing effects? An integrative study of the biological effects of Serjania marginata Casar. (Sapindaceae) in rats. J Ethnopharmacol. 2015;172:312-24.
Périco, L. L., Heredia-Vieira, S. C., Beserra, F. P., de Cássia Dos Santos, R., Weiss, M. B., Resende, F. A., Dos Santos Ramos, M. A., Bonifácio, B. V., Bauab, T. M., Varanda, E. A., de Gobbi, J. I., da Rocha, L. R., Vilegas, W., & Hiruma-Lima, C. A. (2015). Does the gastroprotective action of a medicinal plant ensure healing effects? An integrative study of the biological effects of Serjania marginata Casar. (Sapindaceae) in rats. Journal of Ethnopharmacology, 172, 312-24. https://doi.org/10.1016/j.jep.2015.06.025
Périco LL, et al. Does the Gastroprotective Action of a Medicinal Plant Ensure Healing Effects? an Integrative Study of the Biological Effects of Serjania Marginata Casar. (Sapindaceae) in Rats. J Ethnopharmacol. 2015 Aug 22;172:312-24. PubMed PMID: 26099637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Does the gastroprotective action of a medicinal plant ensure healing effects? An integrative study of the biological effects of Serjania marginata Casar. (Sapindaceae) in rats. AU - Périco,Larissa Lucena, AU - Heredia-Vieira,Silvia Cristina, AU - Beserra,Fernando Pereira, AU - de Cássia Dos Santos,Raquel, AU - Weiss,Marcio Barczyszyn, AU - Resende,Flavia Aparecida, AU - Dos Santos Ramos,Matheus Aparecido, AU - Bonifácio,Bruna Vidal, AU - Bauab,Taís Maria, AU - Varanda,Eliana Aparecida, AU - de Gobbi,Juliana Irani Fratucci, AU - da Rocha,Lúcia Regina Machado, AU - Vilegas,Wagner, AU - Hiruma-Lima,Clélia Akiko, Y1 - 2015/06/20/ PY - 2015/04/06/received PY - 2015/06/03/revised PY - 2015/06/14/accepted PY - 2015/6/24/entrez PY - 2015/6/24/pubmed PY - 2016/6/2/medline KW - Antimicrobial activity KW - Antimutagenic effect KW - Gastroprotective effect KW - Sapindaceae KW - Serjania marginata Casar SP - 312 EP - 24 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 172 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Serjania marginata (Sapindaceae), a medicinal plant commonly found in the Brazilian Cerrado, Paraguay, Bolivia and Argentina, is also known as "cipó-uva" or "cipó-timbó". Ethnopharmacological studies indicate that the leaves from this medicinal plant are used in folk medicine to treat gastric pain. The overall objective of this study was to evaluate the gastroprotective and healing effect of the hydroalcoholic extract obtained from S. marginata (HESM) leaves using rodent experimental models. As part of the integrative study of this medicinal plant, we also evaluated the acute toxicity, antimicrobial, antidiarrheal, (anti)mutagenic, and hemodynamic effects. MATERIAL AND METHODS: We performed a pharmacological study to test the acute toxicity and antimutagenic effect (Ames assay) of the HESM. The HESM was tested against different necrosis-promoting agents and experimental manipulations, such as absolute ethanol, cysteamine, pyloric ligature, and ischemia-reperfusion (I/R) injury. The gastroprotective effect of the HESM was assessed by analyzing the gastric juice (volume, pH, total acidity) and the mucus in the gastric mucosa from rats. We assessed the levels of NO, sulfhydryl compounds, PGE2, vanilloid receptor, glutathione (GSH), and malondialdehyde (MDA), as well as the myeloperoxidase (MPO) activity. The gastric healing effects of the HESM were evaluated during 7 or 14 days of treatment. The intestinal motility, antidiarrheal action, and antibacterial effects (microdilution methods) of the HESM were also evaluated. RESULTS: The phytochemical analysis of the HESM revealed the presence of saponins, flavonoid glycosides, and tannins. The extract exhibited no sign of acute toxicity or mutagenic effect in vitro. In contrast, this extract exhibited a protective effect against the mutagenic action of direct- and indirect-acting mutagens. Only the oral administration of HESM (250mg/kg) significantly decreased the severity of gastric damage induced by ethanol (60.13%) and I/R (58.31%). The HESM exerts its gastroprotective effects by decreasing the MPO and MDA activities in the gastric tissue and by increasing the amount of adherent mucus covering the gastric mucosa. In vitro, the extract also displayed evident antimicrobial effects against Helicobacter pylori. However, the preventive effect of the HESM was not accompanied by an ulcer-healing effect. The treatment with HESM (14 days) significantly increased gastric lesions in 99% of the tested animals compared with the control group. This result represents a highly relevant piece of evidence that should resonate as an alert against the chronic use of this medicinal plant as an antiulcer in folk medicine. CONCLUSIONS: Despite the anti-H. pylori and gastroprotective actions of S. marginata in experimental models, the gastric injuries aggravation induced after chronic treatment with the HESM argues against the use of this plant species in folk medicine. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/26099637/Does_the_gastroprotective_action_of_a_medicinal_plant_ensure_healing_effects_An_integrative_study_of_the_biological_effects_of_Serjania_marginata_Casar___Sapindaceae__in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)00434-1 DB - PRIME DP - Unbound Medicine ER -