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Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections.
PLoS One. 2015; 10(6):e0130378.Plos

Abstract

Acute Respiratory Infections (ARIs) are responsible for considerable morbidity and mortality worldwide. Documentation of respiratory specimens can help for an appropriate clinical management with a significant effect on the disease progress in patient, the antimicrobial therapy used and the risk of secondary spread of infection. Here, we compared the performances of four commercial multiplex kits used in French University Hospital diagnostic microbiology laboratories for the detection of ARI pathogens (i.e., the xTAG Respiratory Viral Panel Fast, RespiFinder SMART 22, CLART PneumoVir and Fast Track Diagnostics Respiratory Pathogen 33 kits). We used a standardised nucleic acids extraction protocol and a comprehensive comparative approach that mixed reference to well established real-time PCR detection techniques and analysis of convergent positive results. We tested 166 respiratory clinical samples and identified a global high degree of correlation for at least three of the techniques (xTAG, RespiFinder and FTD33). For these techniques, the highest Youden's index (YI), positive predictive (PPV) and specificity (Sp) values were observed for Core tests (e.g., influenza A [YI:0.86-1.00; PPV:78.95-100.00; Sp:97.32-100.00] & B [YI:0.44-1.00; PPV:100.00; Sp:100.00], hRSV [YI:0.50-0.99; PPV:85.71-100.00; Sp:99.38-100.00], hMPV [YI:0.71-1.00; PPV:83.33-100.00; Sp:99.37-100.00], EV/hRV [YI:0.62-0.82; PPV:93.33-100.00; Sp:94.48-100.00], AdV [YI:1.00; PPV:100.00; Sp:100.00] and hBoV [YI:0.20-0.80; PPV:57.14-100.00; Sp:98.14-100.00]). The present study completed an overview of the multiplex techniques available for the diagnosis of acute respiratory infections.

Authors+Show Affiliations

Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", Marseille, France.Laboratory of Human and Molecular Virology, IFR146 ICORE, University Hospital, Caen, France.Virology Laboratory, Hôpital Necker Enfants malades, APHP, Université Paris Descartes, Sorbonne Paris Cité-EA 36-20, Paris, France.Unité de Virologie Médicale et Moléculaire, Centre Hospitalier Universitaire, and IFR 53/EA-4303 (DAT/PPCIDH), Faculté de Médecine, Reims, France.UMR-S 707 INSERM, Pierre et Marie Curie University, Paris, France; Public Assistance Hospital of Paris, Department of Internal Medicine, Hôpital Saint Antoine, Unité de Santé Publique, Paris, France.Unité de Virologie Médicale et Moléculaire, Centre Hospitalier Universitaire, and IFR 53/EA-4303 (DAT/PPCIDH), Faculté de Médecine, Reims, France.Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales", Marseille, France; IHU Institute hospitalo-universitaire Méditerranée Infection, APHM Public Hospitals of Marseille, Marseille, France.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

26107509

Citation

Salez, Nicolas, et al. "Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections." PloS One, vol. 10, no. 6, 2015, pp. e0130378.
Salez N, Vabret A, Leruez-Ville M, et al. Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections. PLoS One. 2015;10(6):e0130378.
Salez, N., Vabret, A., Leruez-Ville, M., Andreoletti, L., Carrat, F., Renois, F., & de Lamballerie, X. (2015). Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections. PloS One, 10(6), e0130378. https://doi.org/10.1371/journal.pone.0130378
Salez N, et al. Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections. PLoS One. 2015;10(6):e0130378. PubMed PMID: 26107509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Four Commercial Multiplex Molecular Tests for the Diagnosis of Acute Respiratory Infections. AU - Salez,Nicolas, AU - Vabret,Astrid, AU - Leruez-Ville,Marianne, AU - Andreoletti,Laurent, AU - Carrat,Fabrice, AU - Renois,Fanny, AU - de Lamballerie,Xavier, Y1 - 2015/06/24/ PY - 2014/10/20/received PY - 2015/05/20/accepted PY - 2015/6/25/entrez PY - 2015/6/25/pubmed PY - 2016/3/24/medline SP - e0130378 EP - e0130378 JF - PloS one JO - PLoS One VL - 10 IS - 6 N2 - Acute Respiratory Infections (ARIs) are responsible for considerable morbidity and mortality worldwide. Documentation of respiratory specimens can help for an appropriate clinical management with a significant effect on the disease progress in patient, the antimicrobial therapy used and the risk of secondary spread of infection. Here, we compared the performances of four commercial multiplex kits used in French University Hospital diagnostic microbiology laboratories for the detection of ARI pathogens (i.e., the xTAG Respiratory Viral Panel Fast, RespiFinder SMART 22, CLART PneumoVir and Fast Track Diagnostics Respiratory Pathogen 33 kits). We used a standardised nucleic acids extraction protocol and a comprehensive comparative approach that mixed reference to well established real-time PCR detection techniques and analysis of convergent positive results. We tested 166 respiratory clinical samples and identified a global high degree of correlation for at least three of the techniques (xTAG, RespiFinder and FTD33). For these techniques, the highest Youden's index (YI), positive predictive (PPV) and specificity (Sp) values were observed for Core tests (e.g., influenza A [YI:0.86-1.00; PPV:78.95-100.00; Sp:97.32-100.00] & B [YI:0.44-1.00; PPV:100.00; Sp:100.00], hRSV [YI:0.50-0.99; PPV:85.71-100.00; Sp:99.38-100.00], hMPV [YI:0.71-1.00; PPV:83.33-100.00; Sp:99.37-100.00], EV/hRV [YI:0.62-0.82; PPV:93.33-100.00; Sp:94.48-100.00], AdV [YI:1.00; PPV:100.00; Sp:100.00] and hBoV [YI:0.20-0.80; PPV:57.14-100.00; Sp:98.14-100.00]). The present study completed an overview of the multiplex techniques available for the diagnosis of acute respiratory infections. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/26107509/Evaluation_of_Four_Commercial_Multiplex_Molecular_Tests_for_the_Diagnosis_of_Acute_Respiratory_Infections_ L2 - https://dx.plos.org/10.1371/journal.pone.0130378 DB - PRIME DP - Unbound Medicine ER -