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Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis.
Methods Mol Biol. 2015; 1308:325-46.MM

Abstract

Carrageenans are sulfated galactan isolated from marine red algae with different disaccharide forms. There are also some hybrid carrageenan-like oligomers, which are reported to possess a number of bioactivities. Here, we describe a method to study the structural characterization of a carrageenan-like sulfated galactan FB1 extracted from the red seaweed Furcellaria lumbricalis. We show the process of the general analysis of FB1, including the molecular weight, sulfate content, total sugar content, protein content, and 3,6-anhydrogalactose (3,6-AnG) content analyses. The fine structure identification methods, including desulfation and methylation, nuclear magnetic resonance (NMR), and electrospray ionization collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS), are also described in detail.

Authors+Show Affiliations

Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, 5 Yu Shan Rd., Qingdao, 266003, P.R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26108516

Citation

Lv, Youjing, et al. "Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan From a Marine Red Alga Furcellaria Lumbricalis." Methods in Molecular Biology (Clifton, N.J.), vol. 1308, 2015, pp. 325-46.
Lv Y, Yang B, Zhao X, et al. Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis. Methods Mol Biol. 2015;1308:325-46.
Lv, Y., Yang, B., Zhao, X., Zhang, J., & Yu, G. (2015). Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis. Methods in Molecular Biology (Clifton, N.J.), 1308, 325-46. https://doi.org/10.1007/978-1-4939-2684-8_21
Lv Y, et al. Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan From a Marine Red Alga Furcellaria Lumbricalis. Methods Mol Biol. 2015;1308:325-46. PubMed PMID: 26108516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis. AU - Lv,Youjing, AU - Yang,Bo, AU - Zhao,Xia, AU - Zhang,Junzeng, AU - Yu,Guangli, PY - 2015/6/26/entrez PY - 2015/6/26/pubmed PY - 2016/3/18/medline SP - 325 EP - 46 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1308 N2 - Carrageenans are sulfated galactan isolated from marine red algae with different disaccharide forms. There are also some hybrid carrageenan-like oligomers, which are reported to possess a number of bioactivities. Here, we describe a method to study the structural characterization of a carrageenan-like sulfated galactan FB1 extracted from the red seaweed Furcellaria lumbricalis. We show the process of the general analysis of FB1, including the molecular weight, sulfate content, total sugar content, protein content, and 3,6-anhydrogalactose (3,6-AnG) content analyses. The fine structure identification methods, including desulfation and methylation, nuclear magnetic resonance (NMR), and electrospray ionization collision induced dissociation tandem mass spectrometry (ES-CID-MS/MS), are also described in detail. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/26108516/Structural_Characterization_of_a_Hybrid_Carrageenan_Like_Sulfated_Galactan_from_a_Marine_Red_Alga_Furcellaria_lumbricalis_ L2 - https://dx.doi.org/10.1007/978-1-4939-2684-8_21 DB - PRIME DP - Unbound Medicine ER -