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Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran.
Molecules. 2015 Jun 11; 20(6):10822-38.M

Abstract

Secondary metabolite contents (total phenolic, flavonoid, tocopherol, and tocotrienol) and antioxidant activities of Hashemi rice bran extracts obtained by ultrasound-assisted and traditional solvent (ethanol and 50:50 (v/v) ethanol-water) extraction techniques were compared. Phenolic and, flavonoid compounds were identified using ultra-high performance liquid chromatography and method validation was performed. Significant differences (p < 0.05) were observed among the different extraction techniques upon comparison of phytochemical contents and antioxidant activities. The extracts obtained using the ethanol-water (50:50 v/v) ultrasonic technique showed the highest amounts of total phenolics (288.40 mg/100 g dry material (DM)), total flavonoids (156.20 mg/100 g DM), and total tocotrienols (56.23 mg/100 g DM), and the highest antioxidant activity (84.21% 1,1-diphenyl-2-picrylhydrazyl (DPPH), 65.27% β-carotene-linoleic bleaching and 82.20% nitric oxide scavenging activity). Secondary metabolite contents and antioxidant activities of the rice bran extracts varied depending of the extraction method used, and according to their effectiveness, these were organized in a decreasing order as follows: ethanol-water (50:50 v/v) ultrasonic, ethanol-water (50:50 v/v) maceration, ethanol ultrasonic and ethanol maceration methods. Ferulic, gallic and chlorogenic acids were the most abundant phenolic compounds in rice bran extracts. The phytochemical constituents of Hashemi rice bran and its antioxidant properties provides insights into its potential application to promote health.

Authors+Show Affiliations

Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. alighasemzadeh@upm.edu.my.Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. hawazej@upm.edu.my.Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. ashukur@upm.edu.my.Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. amin_ir@hotmail.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

26111171

Citation

Ghasemzadeh, Ali, et al. "Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran." Molecules (Basel, Switzerland), vol. 20, no. 6, 2015, pp. 10822-38.
Ghasemzadeh A, Jaafar HZ, Juraimi AS, et al. Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran. Molecules. 2015;20(6):10822-38.
Ghasemzadeh, A., Jaafar, H. Z., Juraimi, A. S., & Tayebi-Meigooni, A. (2015). Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran. Molecules (Basel, Switzerland), 20(6), 10822-38. https://doi.org/10.3390/molecules200610822
Ghasemzadeh A, et al. Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran. Molecules. 2015 Jun 11;20(6):10822-38. PubMed PMID: 26111171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative Evaluation of Different Extraction Techniques and Solvents for the Assay of Phytochemicals and Antioxidant Activity of Hashemi Rice Bran. AU - Ghasemzadeh,Ali, AU - Jaafar,Hawa Z E, AU - Juraimi,Abdul Shukor, AU - Tayebi-Meigooni,Amin, Y1 - 2015/06/11/ PY - 2015/04/14/received PY - 2015/06/02/revised PY - 2015/06/04/accepted PY - 2015/6/26/entrez PY - 2015/6/26/pubmed PY - 2016/3/26/medline KW - DPPH KW - Hashemi rice bran KW - nitric oxide scavenging KW - ultra-high performance liquid chromatography KW - ultrasonic KW - β-carotene bleaching SP - 10822 EP - 38 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 20 IS - 6 N2 - Secondary metabolite contents (total phenolic, flavonoid, tocopherol, and tocotrienol) and antioxidant activities of Hashemi rice bran extracts obtained by ultrasound-assisted and traditional solvent (ethanol and 50:50 (v/v) ethanol-water) extraction techniques were compared. Phenolic and, flavonoid compounds were identified using ultra-high performance liquid chromatography and method validation was performed. Significant differences (p < 0.05) were observed among the different extraction techniques upon comparison of phytochemical contents and antioxidant activities. The extracts obtained using the ethanol-water (50:50 v/v) ultrasonic technique showed the highest amounts of total phenolics (288.40 mg/100 g dry material (DM)), total flavonoids (156.20 mg/100 g DM), and total tocotrienols (56.23 mg/100 g DM), and the highest antioxidant activity (84.21% 1,1-diphenyl-2-picrylhydrazyl (DPPH), 65.27% β-carotene-linoleic bleaching and 82.20% nitric oxide scavenging activity). Secondary metabolite contents and antioxidant activities of the rice bran extracts varied depending of the extraction method used, and according to their effectiveness, these were organized in a decreasing order as follows: ethanol-water (50:50 v/v) ultrasonic, ethanol-water (50:50 v/v) maceration, ethanol ultrasonic and ethanol maceration methods. Ferulic, gallic and chlorogenic acids were the most abundant phenolic compounds in rice bran extracts. The phytochemical constituents of Hashemi rice bran and its antioxidant properties provides insights into its potential application to promote health. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/26111171/Comparative_Evaluation_of_Different_Extraction_Techniques_and_Solvents_for_the_Assay_of_Phytochemicals_and_Antioxidant_Activity_of_Hashemi_Rice_Bran_ L2 - http://www.mdpi.com/resolver?pii=molecules200610822 DB - PRIME DP - Unbound Medicine ER -